Programmable nucleases for genome editing

Programmable nucleases for genome editing. From Carroll (2014) Annu. Rev. Biochem. 83: 409.

Model of a pair of ZFNs bound to DNA.

Model of a pair of ZFNs bound to DNA. From Smith et al. (2000) Nucleic Acids Res. 28: 3361.

Illustration of the consequences of targeted genome cleavage

Illustration of the consequences of targeted genome cleavage. After nuclease cleavage at a unique site (red lightning bolt), cells repair the break, sometimes inaccurately (NHEJ), or by copying a template (HDR). From Carroll and Charo (2015) Genome Biol. 16: 242.

 

Genome Editing

Thank you for visiting the Carroll Lab web page. I closed my research lab on July 1, 2018, so there are no research opportunities available with me. I am very grateful to all the people who have worked with me in my 43 years at the University of Utah and contributed to the things we have learned and technologies we have developed in that time.

I plan to remain on the faculty for several more years. I am interested in the broader uses of genome editing and the societal implications of such uses. For example, this powerful technology offers many opportunities for improving food organisms, both crop plants and livestock. Medical applications of genome editing are in their infancy, but also have great promise. We want to ensure that such uses are developed safely and their benefits are distributed appropriately. Complex ethical and societal issues are raised by these prospects, particularly when modifications to the human germline are considered.

Interview with Dana Carroll