Publications

PubMed Listing for Paul Sigala

Plasmodium heme biosynthesis: To be or not to be essential?
Goldberg DE and Sigala PA (2017
PLoS Pathogens, 
13(9): e1006511

In-Cell Enzymology To Probe His–Heme Ligation in Heme Oxygenase Catalysis
Sigala PA, Morante K, Tsumoto K, Caaveiro JM, and Goldberg DE (2016)
Biochemistry, vol 55, 4836−4849

Deconvoluting heme biosynthesis to target blood-stage malaria parasites
Sigala PA, Crowley JR, Henderson JP, Goldberg DE (2015)
LID - 10.7554/eLife.09143. eLife, 4

The heme biosynthesis pathway is essential for Plasmodium falciparum development in mosquito stage but not in blood stages
Ke H*, Sigala PA*, Miura K, Morrisey JM, Mather MW, Crowley JR, Henderson JP, Goldberg DE, Long CA, Vaidya AB (2014)
J Biol Chem, 289(50), 34827-37

The peculiarities and paradoxes of Plasmodium heme metabolism
Sigala PA
, Goldberg DE (2014)
Annu Rev Microbiol, 68, 259-78 [Review]

Direct tests of enzymatic heme degradation by the malaria parasite Plasmodium falciparum
Sigala PA
, Crowley JR, Hsieh S, Henderson JP, Goldberg DE (2012)
J Biol Chem, 287(45), 37793-807

Prior Publications

Determination of Hydrogen Bond Structure in Water versus Aprotic Environments To Test the Relationship Between Length and Stability
Sigala PA
, Ruben EA, Liu CW, Piccoli PM, Hohenstein EG, Martinez TJ, Schultz AJ, Herschlag D (2015)
J Am Chem Soc, 137(17), 5730-40

Quantitative dissection of hydrogen bond-mediated proton transfer in the ketosteroid isomerase active site
Sigala PA*
, Fafarman AT*, Schwans JP*, Fried SD, Fenn TD, Caaveiro JM, Pybus B, Ringe D, Petsko GA, Boxer SG, Herschlag D (2013)
 Proc Natl Acad Sci U S A, 110(28), E2552-61

Quantitative, directional measurement of electric field heterogeneity in the active site of ketosteroid isomerase
Fafarman AT*, Sigala PA*, Schwans JP, Fenn TD, Herschlag D, Boxer SG (2012)
Proc Natl Acad Sci U S A, 109(6), E299-308

Hydrogen bonding in the active site of ketosteroid isomerase: electronic inductive effects and hydrogen bond coupling
Hanoian P, Sigala PA, Herschlag D, Hammes-Schiffer S (2010)
Biochemistry, 49(48), 10339-48

Decomposition of vibrational shifts of nitriles into electrostatic and hydrogen-bonding effects
Fafarman AT, Sigala PA, Herschlag D, Boxer SG (2010)
J Am Chem Soc, 132(37), 12811-3 

Dissecting the paradoxical effects of hydrogen bond mutations in the ketosteroid isomerase oxyanion hole
Kraut DA*, Sigala PA*, Fenn TD, Herschlag D (2010)
Proc Natl Acad Sci U S A, 107(5), 1960-5

Hydrogen bond coupling in the ketosteroid isomerase active site
Sigala PA, Caaveiro JM, Ringe D, Petsko GA, Herschlag D (2009)
Biochemistry, 48(29), 6932-9

Hydrogen bond dynamics in the active site of photoactive yellow protein
Sigala PA
, Tsuchida MA, Herschlag D (2009)
Proc Natl Acad Sci U S A, 106(23), 9232-7

Testing geometrical discrimination within an enzyme active site: constrained hydrogen bonding in the ketosteroid isomerase oxyanion hole
Sigala PA
, Kraut DA, Caaveiro JM, Pybus B, Ruben EA, Ringe D, Petsko GA, Herschlag D (2008)
J Am Chem Soc, 130(41), 13696-708

Do ligand binding and solvent exclusion alter the electrostatic character within the oxyanion hole of an enzymatic active site?
Sigala PA, Fafarman AT, Bogard PE, Boxer SG, Herschlag D (2007)
J Am Chem Soc, 129(40), 12104-5

Testing electrostatic complementarity in enzyme catalysis: hydrogen bonding in the ketosteroid isomerase oxyanion hole
Kraut DA, Sigala PA, Pybus B, Liu CW, Ringe D, Petsko GA, Herschlag D (2006)
PLoS Biol, 4(4), e99

Distinct interaction modes of an AKAP bound to two regulatory subunit isoforms of protein kinase A revealed by amide hydrogen/deuterium exchange
Burns-Hamuro LL, Hamuro Y, Kim JS, Sigala PA, Fayos R, Stranz DD, Jennings PA, Taylor SS, Woods VL Jr (2005)
Protein Sci, 14(12), 2982-92

* Co-authored