Stem Cell Facility

We generate feeder-free induced pluripotent stem cell (iPSC) lines from human blood, skin fibroblasts, and potentially other cell-types.

Current IPSC Approach

Advantages

Our current iPSC approach has several advantages, including:

  • it efficiently reprograms blood and other cell types,
  • it has scalable cell line generation with minimal hands-on time,
  • and it has a non-integrating cytoplasmic RNA vector that rapidly clears.

iPSC Characterization Services

  • Immunostaining of pluripotent markers.
    • Surface markers: TRA-1-60 and SSEA4.
    • Nuclear markers: Nanog, Oct4, Sox2.

  • qPCR analysis of pluripotent markers.
    • Core pluripotent markers upregulated: Nanog, Pou5f1, Sox2, Tfcp2L1.
    • Blood markers silenced: Cd3d, Cd4, Cd8a, Ccr7.
    • Reference markers: Rplp0, Sdha.
  • Mycoplasma and bacteria PCR-based testing (part of a continuous testing plan when generating iPSC lines).
  • Validation of pluripotency: Stemdiff Trilineage Differentiation Kit.
  • Flow Cytometry of pluripotent markers
    • Surface markers: TRA-1-60 and SSEA4.
    • Nuclear markers: Nanog, Oct4, Sox2.

 

iPSC Differentation Services

Methods for differentating IPSCs into a variety of lineages are improving and developing at a rapid pace. Currently we use established protocols that efficiently convert iPSCs into cardiomyocytes/cardiovascular cells. We are exploring options for generating neural lineages. Other cell-type specific protocols may follow. We welcome any inquiries into available resources and will provide basic advice on how to prep iPSCs for differentiation into desired lineages.

 

Affiliated Labs

Additional iPSC characterization and resources will be provided by other core labs.

  • Flow cytometry (Flow Cytometry Core: Please contact James Marvin, jmarvin@cores.utah.edu, 801-585-7382)
  • Molecular Karyotyping (Biorepository and Molecular Pathology Core: Please contact John O'Shea, oshea@hci.utah.edu, 801-213-5645).
  • RNA-seq (Microarray Facility and High-throughput Genomics core: Please contact Brian Dalley, dalley@hci.utah.edu, 801-585-7192).
  • DNA sequencing (DNA sequencing & Genomics Cores: Please contact Derek Warner, dwarner@cores.utah.edu, 801-581-4736).
  • Teratoma assays (Please contact Preclinical Research Resource or Research Histology).
  • Generating Mouse iPSCs (Transgenic Gene-Targeting Mouse Facility, please contact Susan Tamowski, tamowski@genetics.utah.edu, 801-581-3437).

Stem Cell Workshop, ANAT 7760

The Stem Cell Facility is a open resource for assisting faculty, staff, and students in how to properly maintain iPSC lines. If interested in learning more about active stem cell research at the University of Utah and gaining hands-on instruction on culturing iPSCs, please take our stem cell course. A “Stem Cell Workshop” (link to Stem Cell Workshop Page) will be offered again in Spring Semester 2019.

iPSC Advisory Committee

Hilary Coon, PhD
Tom Lane, PhD
John Phillips, PhD
Matthew Rondina, MD, MS
Alex Shcheglovitov, PhD
Martin Tristani-Firouzi, MD

 

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Contact Us

Colin Maguire 
Phone: 801-581-5889
Email

Locations

Main Office

26 N Medical Drive, SLC, UT 84112
Wintrobe, Rm. 203
Monday–Friday: 8am–5pm
801-581-5889
 
DNA Extraction Facility
Wintrobe, Rm. 213
Monday–Friday: 8am–5pm
801-585-3849
 
Stem Cell Facility
Wintrobe, Rm. 208
Monday–Friday: 8am-5pm
801-585-3849