Pemphigoid (Herpes) Gestationis Factor/Complement-Fixing Basement Membrane Zone Antibodies

Ordering Recommendation

• For initial diagnostic assessment and disease monitoring in (herpes) pemphigoid gestationis

• Order concurrently with perilesional skin biopsy for direct immunofluorescence for initial diagnosis of pemphigoid gestationis, also known as herpes gestationis

• Consider additional alternative autoantibody assessment initially and for persisting unexplained or worsening disease activity: Epithelial Antibody Screening Panel


Components

  • Complement-fixing basement membrane zone antibodies by indirect immunofluorescence
  • IgA basement membrane zone antibodies by indirect immunofluorescence
  • IgG basement membrane zone antibodies by indirect immunofluorescence
  • IgG BP 180 and BP 230 antibody levels by ELISAs


Methodology

Indirect immunofluorescence with added fresh human complement - human split skin substrate
Indirect immunofluorescence - monkey esophagus and human split skin substrates
Enzyme-linked immunosorbent assay (ELISA)


Performed

Varies


Reported

3-7 days


New York Department of Health (NYDOH) Approval Status

This test is New York DOH approved.


Information Required to Submit with Order

Completed Immunodermatology Laboratory requisition form and copies of both sides of insurance cards or completed billing information.


CPT Code(s)

88346; 88350 x 2, 83516 (86256 x 3 possible)


SPECIMEN REQUIREMENTS

Collect Blood

Plain red top tube or serum separator tube.  


Specimen Preparation

2 mL serum separated from blood into serum vial provided in kit (Minimum needed: 1.0 mL) 


Storage/Transport Temperature

Room temperature  


Conditions with Disclaimer for Potentially Anomalous Results

Hemolyzed or lipemic serum specimens, whole blood  


Stability

Ambient: 7 days
Refrigerated: 14 days
Frozen: Indefinitely  

Contact Us

Local: 801-581-7139

Toll-Free: 866-266-5699
Fax: 801-585-2202
Email: immunoderm@hsc.utah.edu


Shipping Address

Immunodermatology Laboratory
417 S. Wakara Way, Suite 2151
Salt Lake City, Utah 84108


Hours of Operation MDT

Monday-Friday: 8 am–4:30 pm MDT
Saturday-Sunday: Closed
Holidays: Closed

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