John B. McCullough, PhD

Research Interests

  • Cryogenic Electron Microscopy and Three-Dimensional Reconstruction (cryo-EM)
  • ESCRT Pathway Structure and Function


  • English

Academic Information

  • Departments: Biochemistry - Research Assistant Professor

Academic Office Information

  • 801-585-5139
  • Emma Eccles Jones Medical Research Building
    15 N Medical Dr E
    Salt Lake City, UT

Education History

Type School Degree
Postdoctoral Training University of Utah
Postdoctoral Researcher
Postdoctoral Training
Doctoral Training University of Liverpool
Cellular and Molecular Physiology
Undergraduate National University of Ireland, Galway

Global Impact

Education History

Type School Degree Country
Doctoral Training University of Liverpool
Cellular and Molecular Physiology
Ph.D. United Kingdom
Undergraduate National University of Ireland, Galway
B.Sc. Ireland

Selected Publications

Journal Article

  1. McCullough J, Clippinger AK, Talledge N, Skowyra ML, Saunders MG, Naismith TV, Colf LA, Afonine P, Arthur C, Sundquist WI, Hanson PI, Frost A (2015). Structure and membrane remodeling activity of ESCRT-III helical polymers. Science, 350(6267), 1548-51.
  2. McCullough J, Sundquist WI (2014). Putting a finger in the ring. Nat Struct Mol Biol, 21(12), 1025-7.
  3. Dobro MJ, Samson RY, Yu Z, McCullough J, Ding HJ, Chong PL, Bell SD, Jensen GJ (2013). Electron cryotomography of ESCRT assemblies and dividing Sulfolobus cells suggests that spiraling filaments are involved in membrane scission. Mol Biol Cell, 24(15), 2319-27.
  4. Morita E, Sandrin V, McCullough J, Katsuyama A, Baci Hamilton I, Sundquist WI (2011). ESCRT-III protein requirements for HIV-1 budding. Cell Host Microbe, 9(3), 235-42.
  5. Bajorek M, Schubert HL, McCullough J, Langelier C, Eckert DM, Stubblefield WM, Uter NT, Myszka DG, Hill CP, Sundquist WI (2009). Structural basis for ESCRT-III protein autoinhibition. Nat Struct Mol Biol, 16(7), 754-62.
  6. McCullough J, Fisher RD, Whitby FG, Sundquist WI, Hill CP (2008). ALIX-CHMP4 interactions in the human ESCRT pathway. Proc Natl Acad Sci U S A, 105(22), 7687-91.
  7. Urbe S, McCullough J, Row P, Prior IA, Welchman R, Clague MJ (2006). Control of growth factor receptor dynamics by reversible ubiquitination. Biochem Soc Trans, 34(Pt 5), 754-6.


  1. McCullough J, Frost A, Sundquist WI (2018). Structures, Functions, and Dynamics of ESCRT-III/Vps4 Membrane Remodeling and Fission Complexes.LID - 10.1146/annurev-cellbio-100616-060600 [doi]. [Review]. Annu Rev cell Dev Biol.
  2. McCullough J, Colf LA, Sundquist WI (2013). Membrane fission reactions of the mammalian ESCRT pathway. [Review]. Annu Rev Biochem, 82, 663-92.

Book Chapter

  1. McCullough J, Clague MJ, Urb S (2007). Ubiquitin and protein sorting to the lysosome. In Mayer RJ, Ciechanover A, Rechsteiner M (Eds.), Protein Degradation: Cell Biology of the Ubiquitin-Proteasome System (Volume 3, pp. 76-102). Weinheim: Wiley-VCH.


  1. Talledge N, McCullough J, Wenzel D, Nguyen HC, Lalonde MS, Bajorek M, Skalicky J, Frost A, Sundquist WI (August 7, 2018). The ESCRT-III proteins IST1 and CHMP1B assemble around nucleic acids. BioRxiv (doi: