N. S. Reading, PhD

Languages

  • English

Academic Information

  • Departments: Pathology - Adjunct Assistant Professor
  • Divisions: Clinical Pathology, Hematology/BMT

Email: scott.reading@aruplab.com

Selected Publications

Journal Article

  1. Sirdah MM, Shubair ME, Al-Kahlout MS, Al-Tayeb JM, Prchal JT, Reading NS (2017 Jul). Possible association of 3' UTR +357 A>G, IVS11-nt 93 T>C, c.1311 C>T polymorphism with G6PD deficiency. Hematology, 22(6), 370-374.
  2. Reading NS, Ruiz-Bonilla JA, Christensen RD, Caceres-Perkins W, Prchal JT (2017 May). A patient with both methemoglobinemia and G6PD deficiency: A therapeutic conundrum. Am J Hematol, 92(5), 474-477.
  3. Lanikova L, Reading NS, Hu H, Tashi T, Burjanivova T, Shestakova A, Siwakoti B, Thakur BK, Pun CB, Sapkota A, Abdelaziz S, Feng BJ, Huff CD, Hashibe M, Prchal JT (2017 Feb 14). Evolutionary selected Tibetan variants of HIF pathway and risk of lung cancer. Oncotarget, 8(7), 11739-11747.
  4. Reading NS, Shooter C, Song J, Miller R, Agarwal A, Lanikova L, Clark B, Thein SL, Divoky V, Prchal JT (2016 Nov). Loss of Major DNase I Hypersensitive Sites in Duplicated beta-globin Gene Cluster Incompletely Silences HBB Gene Expression. Hum Mutat, 37(11), 1153-1156.
  5. Agarwal AM, Nussenzveig RH, Reading NS, Patel JL, Sangle N, Salama ME, Prchal JT, Perkins SL, Yaish HM, Christensen RD (2016 Sep). Clinical utility of next-generation sequencing in the diagnosis of hereditary haemolytic anaemias. Br J Haematol, 174(5), 806-14.
  6. Reading NS, Sirdah MM, Shubair ME, Nelson BE, Al-Kahlout MS, Al-Tayeb JM, Aboud LN, Shaban MA, Luzzatto L, Prchal JT (2016 Sep). Favism, the commonest form of severe hemolytic anemia in Palestinian children, varies in severity with three different variants of G6PD deficiency within the same community. Blood Cells Mol Dis, 60, 58-64.
  7. Reading NS, Sirdah MM, Tarazi IS, Prchal JT (2014). Detection of nine Mediterranean beta-thalassemia mutations in Palestinians using three restriction enzyme digest panels: a reliable method for developing countries. Hemoglobin, 38(1), 39-43.
  8. Szankasi P, Reading NS, Vaughn CP, Prchal JT, Bahler DW, Kelley TW (2013 Mar). A quantitative allele-specific PCR test for the BRAF V600E mutation using a single heterozygous control plasmid for quantitation: a model for qPCR testing without standard curves. J Mol Diagn, 15(2), 248-54.
  9. Christensen RD, Lambert DK, Henry E, Eggert LD, Yaish HM, Reading NS, Prchal JT (2013 Feb). Unexplained extreme hyperbilirubinemia among neonates in a multihospital healthcare system. Blood Cells Mol Dis, 50(2), 105-9.
  10. Sirdah M, Reading NS, Vankayalapati H, Perkins SL, Shubair ME, Aboud L, Roper D, Prchal JT (2012 Oct 15-Dec 15). Molecular heterogeneity of glucose-6-phosphate dehydrogenase deficiency in Gaza Strip Palestinians. Blood Cells Mol Dis, 49(3-4), 152-8.
  11. Sirdah M, Reading NS, Perkins SL, Shubair M, Aboud L, Prchal JT (2012 Apr 15). Hemolysis and Mediterranean G6PD mutation (c.563 C>T) and c.1311 C>T polymorphism among Palestinians at Gaza Strip. Blood Cells Mol Dis, 48(4), 203-8.
  12. Nouraie M, Reading NS, Campbell A, Minniti CP, Rana SR, Luchtman-Jones L, Kato GJ, Gladwin MT, Castro OL, Prchal JT, Gordeuk VR (2010 Jul). Association of G6PD with lower haemoglobin concentration but not increased haemolysis in patients with sickle cell anaemia. Br J Haematol, 150(2), 218-25.
  13. Niu X, Nouraie M, Campbell A, Rana S, Minniti CP, Sable C, Darbari D, Dham N, Reading NS, Prchal JT, Kato GJ, Gladwin MT, Castro OL, Gordeuk VR (2009 Nov 23). Angiogenic and inflammatory markers of cardiopulmonary changes in children and adolescents with sickle cell disease. PLoS ONE, 4(11), e7956.
  14. Lippert E, Girodon F, Hammond E, Jelinek J, Reading NS, Fehse B, Hanlon K, Hermans M, Richard C, Swierczek S, Ugo V, Carillo S, Harrivel V, Marzac C, Pietra D, Sobas M, Mounier M, Migeon M, Ellard S, Kroger N, Herrmann R, Prchal JT, Skoda RC, Hermouet S (2009 Jan). Concordance of assays designed for the quantification of JAK2V617F: a multicenter study. Haematologica, 94(1), 38-45.
  15. Reading NS, Lim MS, Elenitoba-Johnson KS (2006). Detection of acquired Janus kinase 2 V617F mutation in myeloproliferative disorders by fluorescence melting curve analysis. Mol Diagn Ther, 10(5), 311-7.
  16. Gouw LG, Reading NS, Jenson SD, Lim MS, Elenitoba-Johnson KS (2005 May). Expression of the Rho-family GTPase gene RHOF in lymphocyte subsets and malignant lymphomas. Br J Haematol, 129(4), 531-3.
  17. Reading NS, Aust SD (2001 Jul 10). Role of disulfide bonds in the stability of recombinant manganese peroxidase. Biochemistry, 40(27), 8161-8.
  18. Reading NS, Aust SD (2000 May-Jun). Engineering a disulfide bond in recombinant manganese peroxidase results in increased thermostability. Biotechnol Prog, 16(3), 326-33.
  19. Timofeevski SL, Nie G, Reading NS, Aust SD (2000 Jan 01). Substrate specificity of lignin peroxidase and a S168W variant of manganese peroxidase. Arch Biochem Biophys, 373(1), 147-53.
  20. Nie G, Reading NS, Aust SD (1999 May 15). Relative stability of recombinant versus native peroxidases from Phanerochaete chrysosporium. Arch Biochem Biophys, 365(2), 328-34.
  21. Timofeevski SL, Nie G, Reading NS, Aust SD (1999 Mar 24). Addition of veratryl alcohol oxidase activity to manganese peroxidase by site-directed mutagenesis. Biochem Biophys Res Commun, 256(3), 500-4.
  22. Reading NS, Aust SD (1998 Nov 15). Effect of modified hemes on the spectral properties and activity of manganese peroxidase. Arch Biochem Biophys, 359(2), 291-6.
  23. Timofeevski SL, Reading NS, Aust SD (1998 Aug 15). Mechanisms for protection against inactivation of manganese peroxidase by hydrogen peroxide. Arch Biochem Biophys, 356(2), 287-95.
  24. Nie G, Reading NS, Aust SD (1998 Aug 10). Expression of the lignin peroxidase H2 gene from Phanerochaete chrysosporium in Escherichia coli. Biochem Biophys Res Commun, 249(1), 146-50.

Review

  1. Sirdah MM, Al-Kahlout MS, Reading NS (2016 Sep). National G6PD neonatal screening program in Gaza Strip of Palestine: rationale, challenges and recommendations. [Review]. Clin Genet, 90(3), 191-8.

Case Report

  1. Agarwal N, Kutlar F, Mojica-Henshaw MP, Ou CN, Gaikwad A, Reading NS, Bailey L, Kutlar A, Prchal JT (2007 Dec). Missense mutation of the last nucleotide of exon 1 (G->C) of beta globin gene not only leads to undetectable mutant peptide and transcript but also interferes with the expression of wild allele. Haematologica, 92(12), 1715-6.
  2. Reading NS, Jenson SD, Smith JK, Lim MS, Elenitoba-Johnson KS (2003 May). 5'-(RACE) identification of rare ALK fusion partner in anaplastic large cell lymphoma. J Mol Diagn, 5(2), 136-40.

Letter

  1. Salama ME, Swierczek SI, Tashi T, Warby CA, Reading NS, Prchal JT (2014 Sep 04). Calreticulin mutated prefibrotic-stage myelofibrosis and PMF represent an independent clone from coexisting CLL [Letter to the editor]. Blood, 124(10), 1691-2.
  2. Nussenzveig RH, Vanhille DL, Hussey D, Reading NS, Agarwal AM (2012 Oct). Development of a rapid multiplex PCR assay for identification of the three common Hemoglobin-Lepore variants (Boston-Washington, Baltimore, and Hollandia) and identification of a new Lepore variant [Letter to the editor]. Am J Hematol, 87(10), E74-5.
  3. Olcaydu D, Skoda RC, Looser R, Li S, Cazzola M, Pietra D, Passamonti F, Lippert E, Carillo S, Girodon F, Vannucchi A, Reading NS, Prchal JT, Ay C, Pabinger I, Gisslinger H, Kralovics R (2009 Oct). The 'GGCC' haplotype of JAK2 confers susceptibility to JAK2 exon 12 mutation-positive polycythemia vera [Letter to the editor]. Leukemia, 23(10), 1924-6.