Dana Carroll

Dana Carroll, PhD

Languages spoken: English

Academic Information

Departments Primary - Biochemistry

Lab

Dana Carroll, PhD, is a Distinguished Professor in the Department of Biochemistry at the University of Utah School of Medicine.

Carroll’s research involves genome engineering using targetable nucleases. His lab pioneered the development of zinc-finger nucleases as gene targeting tools and continued working with the more recent TALENs and CRISPR/Cas nucleases. Much of the effort was focused on optimizing the efficiency of these reagents for targeted mutagenesis and gene replacement, which could ultimately provide treatment for many diseases, including certain types of cancer. This technology has now been applied to more than 200 different organisms, including current clinical trials in humans and improvements in crop plants and livestock. Carroll no longer has an active research laboratory, but he maintains an interest in the applications and societal implications of genome editing.

Carroll received his bachelor’s degree from Swarthmore College, Pennsylvania, and his PhD from the University of California, Berkeley. He did postdoctoral research at the Beatson Institute for Cancer Research in Glasgow, Scotland, and at the Carnegie Institution of Washington Department of Embryology in Baltimore.

Education History

Undergraduate Swarthmore College
 BA
Doctoral Training University of California, Berkeley
 PhD
Postdoctoral Fellowship Beatson Institute for Cancer Research
 Postdoctoral Fellow
Postdoctoral Fellowship Carnegie Institution of Washington
 Postdoctoral Fellow

Selected Publications

Journal Article

  1. Cho SW, Lee J, Carroll D, Kim JS, Lee (2013). Heritable gene knockout in Caenorhabditis elegans by direct injection of Cas9-sgRNA ribonucleoproteins. Genetics, 195(3), 1177-80.
  2. Sarno R, Vicq Y, Uematsu N, Luka M, Lapierre C, Carroll D, Bastianelli G, Serero A, Nicolas (2017). Programming sites of meiotic crossovers using Spo11 fusion proteins. Nucleic acids research, 45(19), e164.
  3. Yarrington RM, Verma S, Schwartz S, Trautman JK, Carroll (2018). Nucleosomes inhibit target cleavage by CRISPR-Cas9 in vivo.LID - 201810062 [pii]LID - 10.1073/pnas.1810062115 [doi]. Proceedings of the National Academy of Sciences of the United States of America, 115, 9351-9358.
  4. Beumer KJ, Carroll (2014). Targeted genome engineering techniques in Drosophila. Methods (San Diego, Calif.), 68(1), 29-37.
  5. Carroll D, Beumer K (2014). Genome engineering with TALENs and ZFNs: repair pathways and donor design. Methods (San Diego, Calif.), 69(2), 137-41.
  6. DeWitt MA, Corn JE, Carroll (2017). Genome editing via delivery of Cas9 ribonucleoprotein. Methods (San Diego, Calif.), 121-122, 9-15.
  7. DeWitt MA, Magis W, Bray NL, Wang T, Berman JR, Urbinati F, Heo SJ, Mitros T, Munoz DP, Boffelli D, Kohn DB, Walters MC, Carroll D, Martin DI, Corn J (2016). Selection-free genome editing of the sickle mutation in human adult hematopoietic stem/progenitor cells. Science translational medicine, 8(360), 360ra134.
  8. Beumer KJ, Trautman JK, Mukherjee K, Carroll (2013). Donor DNA Utilization during Gene Targeting with Zinc-finger Nucleases.LID - g3.112.005439v2 [pii]LID - 10.1534/g3.112.005439 [doi]. G3 (Bethesda, Md.), 3, 657-664.
  9. Beumer KJ, Trautman JK, Christian M, Dahlem TJ, Lake CM, Hawley RS, Grunwald DJ, Voytas DF, Carroll (2013). Comparing zinc finger nucleases and transcription activator-like effector nucleases for gene targeting in Drosophila. G3 (Bethesda, Md.), 3(10), 1717-25.
  10. Wilson RC, Carroll (2019). The Daunting Economics of Therapeutic Genome Editing. CRISPR J, 2(5), 280-284.
  11. Christensen S, Pont-Kingdon G, Carroll (2000). Target specificity of the endonuclease from the Xenopus laevis non-long terminal repeat retrotransposon, Tx1L. Molecular and cellular biology, 20(4), 1219-26.
  12. Bibikova M, Wu B, Chi E, Kim KH, Trautman JK, Carroll (1998). Characterization of FEN-1 from Xenopus laevis. cDNA cloning and role in DNA metabolism. The Journal of biological chemistry, 273(51), 34222-9.
  13. Segal DJ, Faruqi AF, Glazer PM, Carroll (1997). Processing of targeted psoralen cross-links in Xenopus oocytes. Molecular and cellular biology, 17(11), 6645-52.
  14. Pont-Kingdon G, Chi E, Christensen S, Carroll (1997). Ribonucleoprotein formation by the ORF1 protein of the non-LTR retrotransposon Tx1L in Xenopus oocytes. Nucleic acids research, 25(15), 3088-94.
  15. Segal DJ, Carroll (1995). Endonuclease-induced, targeted homologous extrachromosomal recombination in Xenopus oocytes. Proceedings of the National Academy of Sciences of the United States of America, 92(3), 806-10.
  16. Lehman CW, Jeong-Yu S, Trautman JK, Carroll (1994). Repair of heteroduplex DNA in Xenopus laevis oocytes. Genetics, 138(2), 459-70.
  17. Carroll D, Lehman CW, Jeong-Yu S, Dohrmann P, Dawson RJ, Trautman J (1994). Distribution of exchanges upon homologous recombination of exogenous DNA in Xenopus laevis oocytes. Genetics, 138(2), 445-57.
  18. Lehman CW, Trautman JK, Carroll (1994). Illegitimate recombination in Xenopus: characterization of end-joined junctions. Nucleic acids research, 22(3), 434-42.
  19. Lehman CW, Clemens M, Worthylake DK, Trautman JK, Carroll (1993). Homologous and illegitimate recombination in developing Xenopus oocytes and eggs. Molecular and cellular biology, 13(11), 6897-906.
  20. Lehman CW, Carroll (1993). Isolation of large quantities of functional, cytoplasm-free Xenopus laevis oocyte nuclei. Analytical biochemistry, 211(2), 311-9.
  21. Jeong-Yu S, Carroll (1992). Effect of terminal nonhomologies on homologous recombination in Xenopus laevis oocytes. Molecular and cellular biology, 12(12), 5426-37.
  22. Lehman CW, Carroll (1991). Homologous recombination catalyzed by a nuclear extract from Xenopus oocytes. Proceedings of the National Academy of Sciences of the United States of America, 88(23), 10840-4.
  23. Maryon E, Carroll (1991). Characterization of recombination intermediates from DNA injected into Xenopus laevis oocytes: evidence for a nonconservative mechanism of homologous recombination. Molecular and cellular biology, 11(6), 3278-87.
  24. Maryon E, Carroll (1991). Involvement of single-stranded tails in homologous recombination of DNA injected into Xenopus laevis oocyte nuclei. Molecular and cellular biology, 11(6), 3268-77.
  25. Carroll D, Lehman C (1991). DNA recombination and repair in oocytes, eggs, and extracts. Methods in cell biology, 36, 467-86.
  26. Urnes MS, Carroll (1990). Amylase synthesis as a simple model system for translation and hybrid arrest in Xenopus oocytes. Gene, 95(2), 267-74.
  27. Sweigert SE, Carroll (1990). Repair and recombination of X-irradiated plasmids in Xenopus laevis oocytes. Molecular and cellular biology, 10(11), 5849-56.
  28. Maryon E, Carroll (1989). Degradation of linear DNA by a strand-specific exonuclease activity in Xenopus laevis oocytes. Molecular and cellular biology, 9(11), 4862-71.
  29. Garrett JE, Knutzon DS, Carroll (1989). Composite transposable elements in the Xenopus laevis genome. Molecular and cellular biology, 9(7), 3018-27.

Review

  1. Carroll (2014). Genome engineering with targetable nucleases. Annual review of biochemistry, 83, 409-39.
  2. Carroll (2015). Genome editing by targeted chromosomal mutagenesis. Methods in molecular biology (Clifton, N.J.), 1239, 1-13.
  3. Chandrasegaran S, Carroll (2015). Origins of Programmable Nucleases for Genome Engineering.LID - S0022-2836(15)00606-3 [pii]LID - 10.1016/j.jmb.2015.10.014 [doi]. Journal of molecular biology, 428, 963-89.
  4. Carroll (2017). Genome Editing: Past, Present, and Future. The Yale journal of biology and medicine, 90(4), 653-659.
  5. Carroll D, Charo R (2015). The societal opportunities and challenges of genome editing. Genome biology, 16(1), 242.
  6. Pauwels K, Podevin N, Breyer D, Carroll D, Herman (2014). Engineering nucleases for gene targeting: safety and regulatory considerations. New biotechnology, 31(1), 18-27.
  7. Carroll (2023). RNA in Therapeutics: CRISPR in the Clinic. Molecules and cells, 46(1), 4-9.
  8. Carroll (2021). A short, idiosyncratic history of genome editing. Gene and genome editing, 1, 100002.

Commentary

  1. Chandrasegaran S, Bullen CK, Carroll (2017). Genome editing of human embryos: to edit or not to edit, that is the question. The Journal of clinical investigation, 127(10), 3588-3590.
  2. Baltimore D, Berg P, Botchan M, Carroll D, Charo RA, Church G, Corn JE, Daley GQ, Doudna JA, Fenner M, Greely HT, Jinek M, Martin GS, Penhoet E, Puck J, Sternberg SH, Weissman JS, Yamamoto K (2015). Biotechnology. A prudent path forward for genomic engineering and germline gene modification. Science (New York, N.Y.), 348(6230), 36-8.
  3. Carroll (2018). p53 Throws CRISPR a Curve. Trends in pharmacological sciences, 39(9), 783-784.
  4. Carroll (2014). Precision genome engineering. Current biology, 24(3), R102-3.
  5. Carroll (2013). Staying on target with CRISPR-Cas. Nature biotechnology, 31(9), 807-9.
  6. Bosley KS, Botchan M, Bredenoord AL, Carroll D, Charo RA, Charpentier E, Cohen R, Corn J, Doudna J, Feng G, Greely HT, Isasi R, Ji W, Kim JS, Knoppers B, Lanphier E, Li J, Lovell-Badge R, Martin GS, Moreno J, Naldini L, Pera M, Perry AC, Venter JC, Zhang F, Zhou (2015). CRISPR germline engineering--the community speaks. Nature biotechnology, 33(5), 478-86.
  7. Carroll (2012). A CRISPR approach to gene targeting. Molecular therapy, 20(9), 1658-60.
  8. Carroll (2016). A Perspective on the State of Genome Editing. Molecular therapy, 24(3), 412-3.
  9. Carroll (2016). Genome editing: progress and challenges for medical applications. Genome medicine, 8(1), 120.
  10. Carroll (2022). Rewriting Nature: The case of heritable human genome editing. Boston University Law Review Online, 102, 1-6.
  11. Carroll D, Meyer B (2021). Life 2.0 - A CRISPR approach to a sustainable planet. Proceedings of the National Academy of Sciences of the United States of America, 118(22), e2107418118.

Editorial

  1. Carroll (2019). Collateral damage: benchmarking off-target effects in genome editing. Genome biology, 20(1), 114.

Letter

  1. Carroll D, Van Eenennaam AL, Taylor JF, Seger J, Voytas D (2016). Regulate genome-edited products, not genome editing itself. Nature biotechnology, 34(5), 477-9.
  2. Urnov FD, Ronald PC, Carroll (2018). A call for science-based review of the European court's decision on gene-edited crops. Nature biotechnology, 36(9), 800-802.
  3. Hackett P, Carroll (2015). Regulatory hurdles for agriculture GMOs. Science (New York, N.Y.), 347(6228), 1324.