H. Joseph Yost

H. Joseph Yost, PhD

Languages spoken: English

Academic Information

Departments Adjunct - Pediatrics , Primary - Neurobiology

Divisions: Hematology/Oncology

Lab

H. Joseph Yost, Ph.D. is Vice Chairman for Basic Science Research, Department of Pediatrics, Richard L. Stimson Presidential Endowed Chair, and Professor of Neurobiology & Anatomy

Dr. Yost works at the intersection between model organism genetics and the discovery of novel disease-causing mutations in human genomes. His long-term research goal is to understand the gene regulatory networks and developmental mechanisms that assign different cell identities in functionally appropriate positions in the vertebrate embryo, and to utilize this knowledge for the advancement of human medicine. This includes building bioinformatics toolsthat are disease agnostic and species agnostic, that we have applied to human genomes as well as model organism genomes. His lab is recognized as a founder and leader in the field of vertebrate left-right (LR) development, discovering genetic pathways and mechanisms that convert bilateral symmetry to left-right asymmetry, including essential functional and/or structural asymmetries in the heart, brain and digestive system. Disruptions of LR asymmetry result in a large percentage of complex congenital heart defects affecting approximately thirty-five thousand births per year in the US. His lab has generated zebrafish genetic models of human congenital heart disease (CHD), ciliopathies, Kabuki syndrome, Roberts syndrome, Li-Fraumeni syndrome and rare/orphan diseases in pediatrics.

Education History

Undergraduate Creighton University
 BS
Doctoral Training University of Chicago
 PhD
Postdoctoral Fellowship University of California, Berkeley
 Postdoctoral Fellow
Other Training University of Utah School of Business
 Certificate

Selected Publications

Journal Article

  1. O'Connor TM, Guaman MC, Randell KA, Keenan HT, Snowden J, Mack JW, Camp EA, Perez O, Chang ML, Myers AL, Nigrovic LE, O'Toole J, Reed JL, Reese J, Rosenberg AR, Slater AC, Wootton SH, Ziniel SI, Yost HJ, Murray KO, Shekerdemian L, Chumpitazi C (2024). Impact of the COVID-19 pandemic on pediatric faculty: a report from nine academic institutions. Pediatric research, 95(3), 775-784.
  2. Eisa-Beygi S, Hu MM, Kumar SN, Jeffery BE, Collery RF, Vo NJ, Lamichanne BS, Yost HJ, Veldman MB, Link B (2023). Mesenchymal Stromal Cells Facilitate Tip Cell Fusion Downstream of BMP-Mediated Venous Angiogenesis-Brief Report. Arteriosclerosis, thrombosis, and vascular biology, 43(7), e231-e237.
  3. Casey MA, Hill JT, Hoshijima K, Bryan CD, Gribble SL, Brown JT, Chien CB, Yost HJ, Kwan K (2022). Shutdown corner, a large deletion mutant isolated from a haploid mutagenesis screen in zebrafish. G3 (Bethesda, Md.), 12(3),
  4. Weso'owski S, Lemmon G, Hernandez EJ, Henrie A, Miller TA, Weyhrauch D, Puchalski MD, Bray BE, Shah RU, Deshmukh VG, Delaney R, Yost HJ, Eilbeck K, Tristani-Firouzi M, Yandell (2022). An explainable artificial intelligence approach for predicting cardiovascular outcomes using electronic health records. PLOS digital health, 1(1),
  5. Yates JD, Russell RC, Barton NJ, Yost HJ, Hill J (2021). A simple and rapid method for enzymatic synthesis of CRISPR-Cas9 sgRNA libraries. Nucleic acids research, 49(22), e131.
  6. Parvez S, Herdman C, Beerens M, Chakraborti K, Harmer ZP, Yeh JJ, MacRae CA, Yost HJ, Peterson R (2021). MIC-Drop: A platform for large-scale in vivo CRISPR screens. Science (New York, N.Y.), 373(6559), 1146-1151.
  7. Ward T, Tai W, Morton S, Impens F, Van Damme P, Van Haver D, Timmerman E, Venturini G, Zhang K, Jang MY, Willcox JAL, Haghighi A, Gelb BD, Chung WK, Goldmuntz E, Porter GA Jr, Lifton RP, Brueckner M, Yost HJ, Bruneau BG, Gorham J, Kim Y, Pereira A, Homsy J, Benson CC, DePalma SR, Varland S, Chen CS, Arnesen T, Gevaert K, Seidman C, Seidman J (2021). Mechanisms of Congenital Heart Disease Caused by NAA15 Haploinsufficiency. Circulation research, 128(8), 1156-1169.
  8. Capasso TL, Li B, Volek HJ, Khalid W, Rochon ER, Anbalagan A, Herdman C, Yost HJ, Villanueva FS, Kim K, Roman B (2020). BMP10-mediated ALK1 signaling is continuously required for vascular development and maintenance. Angiogenesis, 23(2), 203-220.
  9. Edwards JJ, Rouillard AD, Fernandez NF, Wang Z, Lachmann A, Shankaran SS, Bisgrove BW, Demarest B, Turan N, Srivastava D, Bernstein D, Deanfield J, Giardini A, Porter G, Kim R, Roberts AE, Newburger JW, Goldmuntz E, Brueckner M, Lifton RP, Seidman CE, Chung WK, Tristani-Firouzi M, Yost HJ, Ma'ayan A, Gelb B (2020). Systems Analysis Implicates WAVE2 Complex in the Pathogenesis of Developmental Left-Sided Obstructive Heart Defects. JACC. Basic to translational science, 5(4), 376-386.
  10. Jurynec MJ, Bai X, Bisgrove BW, Jackson H, Nechiporuk A, Palu RAS, Grunwald HA, Su YC, Hoshijima K, Yost HJ, Zon LI, Grunwald D (2019). The Paf1 complex and P-TEFb have reciprocal and antagonist roles in maintaining multipotent neural crest progenitors. Development (Cambridge, England), 146(24),
  11. Watkins WS, Hernandez EJ, Wesolowski S, Bisgrove BW, Sunderland RT, Lin E, Lemmon G, Demarest BL, Miller TA, Bernstein D, Brueckner M, Chung WK, Gelb BD, Goldmuntz E, Newburger JW, Seidman CE, Shen Y, Yost HJ, Yandell M, Tristani-Firouzi (2019). De novo and recessive forms of congenital heart disease have distinct genetic and phenotypic landscapes. Nature communications, 10(1), 4722.
  12. Serrano MLA, Demarest BL, Tone-Pah-Hote T, Tristani-Firouzi M, Yost H (2019). Inhibition of Notch signaling rescues cardiovascular development in Kabuki Syndrome. PLoS biology, 17(9), e3000087.
  13. Abdul-Wajid S, Demarest BL, Yost H (2018). Loss of embryonic neural crest derived cardiomyocytes causes adult onset hypertrophic cardiomyopathy in zebrafish. Nature communications, 9(1), 4603.
  14. Karanth S, Adams JD, Serrano MLA, Quittner-Strom EB, Simcox J, Villanueva CJ, Ozcan L, Holland WL, Yost HJ, Vella A, Schlegel (2018). A Hepatocyte FOXN3-¿ Cell Glucagon Axis Regulates Fasting Glucose. Cell reports, 24(2), 312-319.
  15. Matsunami N, Shanmugam H, Baird L, Stevens J, Byrne JL, Barnhart DC, Rau C, Feldkamp ML, Yoder BA, Leppert MF, Yost HJ, Brunelli (2018). Germline but not somatic de novo mutations are common in human congenital diaphragmatic hernia. Birth defects research, 110(7), 610-617.
  16. Bisgrove BW, Su YC, Yost H (2017). Maternal Gdf3 is an obligatory cofactor in Nodal signaling for embryonic axis formation in zebrafish. eLife, 6,
  17. Jin SC, Homsy J, Zaidi S, Lu Q, Morton S, DePalma SR, Zeng X, Qi H, Chang W, Sierant MC, Hung WC, Haider S, Zhang J, Knight J, Bjornson RD, Castaldi C, Tikhonoa IR, Bilguvar K, Mane SM, Sanders SJ, Mital S, Russell MW, Gaynor JW, Deanfield J, Giardini A, Porter GA Jr, Srivastava D, Lo CW, Shen Y, Watkins WS, Yandell M, Yost HJ, Tristani-Firouzi M, Newburger JW, Roberts AE, Kim R, Zhao H, Kaltman JR, Goldmuntz E, Chung WK, Seidman JG, Gelb BD, Seidman CE, Lifton RP, Brueckner (2017). Contribution of rare inherited and de novo variants in 2,871 congenital heart disease probands. Nature genetics, 49(11), 1593-1601.
  18. Hill JT, Demarest B, Gorsi B, Smith M, Yost H (2017). Heart morphogenesis gene regulatory networks revealed by temporal expression analysis. Development (Cambridge, England), 144(19), 3487-3498.
  19. Shankaran SS, Dahlem TJ, Bisgrove BW, Yost HJ, Tristani-Firouzi (2017). CRISPR/Cas9-Directed Gene Editing for the Generation of Loss-of-Function Mutants in High-Throughput Zebrafish F(0) Screens. Current protocols in molecular biology, 119, 31.9.1-31.9.22.
  20. Lyozin GT, Kosaka Y, Bhattacharje G, Yost HJ, Brunelli (2017). Direct Isolation of Seamless Mutant Bacterial Artificial Chromosomes. Current protocols in molecular biology, 118, 8.6.1-8.6.29.
  21. Gittenberger-de Groot AC, Hoppenbrouwers T, Miquerol L, Kosaka Y, Poelmann RE, Wisse LJ, Yost HJ, Jongbloed MR, Deruiter MC, Brunelli (2016). 14-3-3epsilon controls multiple developmental processes in the mouse heart. Developmental dynamics, 245(11), 1107-1123.
  22. Merchant SS, Kosaka Y, Yost HJ, Hsu EW, Brunelli (2016). Micro-Computed Tomography for the Quantitative 3-Dimensional Assessment of the Compact Myocardium in the Mouse Embryo. Circulation journal, 80(8), 1795-803.
  23. Karanth S, Zinkhan EK, Hill JT, Yost HJ, Schlegel (2016). FOXN3 Regulates Hepatic Glucose Utilization. Cell reports, 15(12), 2745-55.
  24. Bowles NE, Jou CJ, Arrington CB, Kennedy BJ, Earl A, Matsunami N, Meyers LL, Etheridge SP, Saarel EV, Bleyl SB, Yost HJ, Yandell M, Leppert MF, Tristani-Firouzi M, Gruber PJ, Baylor Hopkins Centers for Mendelian Genomic (2015). Exome analysis of a family with Wolff-Parkinson-White syndrome identifies a novel disease locus. American journal of medical genetics. Part A, 167A(12), 2975-84.
  25. Percival SM, Thomas HR, Amsterdam A, Carroll AJ, Lees JA, Yost HJ, Parant J (2015). Variations in dysfunction of sister chromatid cohesion in esco2 mutant zebrafish reflect the phenotypic diversity of Roberts syndrome. Disease models & mechanisms, 8(8), 941-55.
  26. Nash D, Arrington CB, Kennedy BJ, Yandell M, Wu W, Zhang W, Ware S, Jorde LB, Gruber PJ, Yost HJ, Bowles NE, Bleyl S (2015). Shared Segment Analysis and Next-Generation Sequencing Implicates the Retinoic Acid Signaling Pathway in Total Anomalous Pulmonary Venous Return (TAPVR). PloS one, 10(6), e0131514.
  27. Hill JT, Demarest BL, Bisgrove BW, Su YC, Smith M, Yost H (2014). Poly peak parser: Method and software for identification of unknown indels using sanger sequencing of polymerase chain reaction products. Developmental dynamics, 243(12), 1632-6.
  28. Lyozin GT, Bressloff PC, Kumar A, Kosaka Y, Demarest BL, Yost HJ, Kuehn MR, Brunelli (2014). Isolation of rare recombinants without using selectable markers for one-step seamless BAC mutagenesis. Nature methods, 11(9), 966-970.
  29. Neugebauer JM, Yost H (2014). FGF signaling is required for brain left-right asymmetry and brain midline formation. Developmental biology, 386(1), 123-34.
  30. Samson SC, Ferrer T, Jou CJ, Sachse FB, Shankaran SS, Shaw RM, Chi NC, Tristani-Firouzi M, Yost H (2013). 3-OST-7 regulates BMP-dependent cardiac contraction. PLoS biology, 11(12), e1001727.
  31. Arrington CB, Peterson AG, Yost H (2013). Sdc2 and Tbx16 regulate Fgf2-dependent epithelial cell morphogenesis in the ciliated organ of asymmetry. Development (Cambridge, England), 140(19), 4102-9.
  32. Peterson AG, Wang X, Yost H (2013). Dvr1 transfers left-right asymmetric signals from Kupffer's vesicle to lateral plate mesoderm in zebrafish. Developmental biology, 382(1), 198-208.
  33. Neugebauer JM, Cadwallader AB, Amack JD, Bisgrove BW, Yost H (2013). Differential roles for 3-OSTs in the regulation of cilia length and motility. Development (Cambridge, England), 140(18), 3892-902.
  34. Hill JT, Demarest BL, Bisgrove BW, Gorsi B, Su YC, Yost H (2013). MMAPPR: mutation mapping analysis pipeline for pooled RNA-seq. Genome research, 23(4), 687-97.
  35. Maguire CT, Demarest BL, Hill JT, Palmer JD, Brothman AR, Yost HJ, Condic M (2013). Genome-wide analysis reveals the unique stem cell identity of human amniocytes. PloS one, 8(1), e53372.
  36. Arrington CB, Bleyl SB, Matsunami N, Bowles NE, Leppert TI, Demarest BL, Osborne K, Yoder BA, Byrne JL, Schiffman JD, Null DM, DiGeronimo R, Rollins M, Faix R, Comstock J, Camp NJ, Leppert MF, Yost HJ, Brunelli (2012). A family-based paradigm to identify candidate chromosomal regions for isolated congenital diaphragmatic hernia. American journal of medical genetics. Part A, 158A(12), 3137-47.
  37. Kosaka Y, Cieslik KA, Li L, Lezin G, Maguire CT, Saijoh Y, Toyo-oka K, Gambello MJ, Vatta M, Wynshaw-Boris A, Baldini A, Yost HJ, Brunelli (2012). 14-3-3¿ plays a role in cardiac ventricular compaction by regulating the cardiomyocyte cell cycle. Molecular and cellular biology, 32(24), 5089-102.
  38. Cadwalader EL, Condic ML, Yost H (2012). 2-O-sulfotransferase regulates Wnt signaling, cell adhesion and cell cycle during zebrafish epiboly. Development (Cambridge, England), 139(7), 1296-305.
  39. Bisgrove BW, Makova S, Yost HJ, Brueckner (2012). RFX2 is essential in the ciliated organ of asymmetry and an RFX2 transgene identifies a population of ciliated cells sufficient for fluid flow. Developmental biology, 363(1), 166-78.
  40. Wythe JD, Jurynec MJ, Urness LD, Jones CA, Sabeh MK, Werdich AA, Sato M, Yost HJ, Grunwald DJ, Macrae CA, Li D (2011). Hadp1, a newly identified pleckstrin homology domain protein, is required for cardiac contractility in zebrafish. Disease models & mechanisms, 4(5), 607-21.
  41. Lezin G, Kosaka Y, Yost HJ, Kuehn MR, Brunelli (2011). A one-step miniprep for the isolation of plasmid DNA and lambda phage particles. PloS one, 6(8), e23457.
  42. Parant JM, George SA, Holden JA, Yost H (2010). Genetic modeling of Li-Fraumeni syndrome in zebrafish. Disease models & mechanisms, 3(1-2), 45-56.
  43. Parant JM, George SA, Pryor R, Wittwer CT, Yost H (2009). A rapid and efficient method of genotyping zebrafish mutants. Developmental dynamics, 238(12), 3168-74.
  44. Arrington CB, Yost H (2009). Extra-embryonic syndecan 2 regulates organ primordia migration and fibrillogenesis throughout the zebrafish embryo. Development (Cambridge, England), 136(18), 3143-52.
  45. Neugebauer JM, Amack JD, Peterson AG, Bisgrove BW, Yost H (2009). FGF signalling during embryo development regulates cilia length in diverse epithelia. Nature, 458(7238), 651-4.
  46. Wang X, Yost H (2008). Initiation and propagation of posterior to anterior (PA) waves in zebrafish left-right development. Developmental dynamics, 237(12), 3640-7.
  47. Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Parant JM, Yost HJ, Kanki JP, Chien C (2007). The Tol2kit: a multisite gateway-based construction kit for Tol2 transposon transgenesis constructs. Developmental dynamics, 236(11), 3088-99.
  48. Amack JD, Wang X, Yost H (2007). Two T-box genes play independent and cooperative roles to regulate morphogenesis of ciliated Kupffer's vesicle in zebrafish. Developmental biology, 310(2), 196-210.

Review

  1. Poulain FE, Yost H (2015). Heparan sulfate proteoglycans: a sugar code for vertebrate development?. Development (Cambridge, England), 142(20), 3456-67.
  2. Kramer KL, Yost H (2002). Cardiac left-right development: are the early steps conserved?. Cold Spring Harbor symposia on quantitative biology, 67, 37-43.

Newspaper

  1. Sable C, Li JS, Tristani-Firouzi M, Fagerlin A, Silver RM, Yandel M, Yost HJ, Beaton A, Dale J, Engel M, Watkins D, Spurney C, Skinner AC, Armstrong SC, Shah SH, Allen N, Davis M, Hou L, Van Horn L, Labarthe D, Lloyd-Jones D, Marino (2023). American Heart Association's Children's Strategically Focused Research Network Experience. Journal of the American Heart Association, 12(7), e028356.