Jesse Rowley

Jesse Rowley, PhD

Languages spoken: English

Academic Information

Departments Primary - Internal Medicine

Divisions: Hematology & Hematologic Malignancies

Lab

Research Statement

My research objective is to understand how platelets and megakaryocytes influence human health and disease. Our work integrates cutting-edge multi-omics approaches with traditional molecular, cellular, and in vivo approaches to define mechanisms of platelet function, and uncover new avenues for platelet diagnostics and therapeutics.

Education History

Undergraduate Brigham Young University
 BS
Doctoral Training The Johns Hopkins University
 PhD
Research Fellow Johns Hopkins University
 Research Fellow
Postdoctoral Fellowship The Johns Hopkins University
 Postdoctoral Fellow
Research Fellow University of Utah
 Postdoctoral Research Fellow

Selected Publications

Journal Article

  1. Pawlinski R, Wang JG, Owens AP 3rd, Williams J, Antoniak S, Tencati M, Luther T, Rowley JW, Low EN, Weyrich AS, Mackman (2010). Hematopoietic and nonhematopoietic cell tissue factor activates the coagulation cascade in endotoxemic mice. Blood, 116(5), 806-14.
  2. Rowley JW, Oler AJ, Tolley ND, Hunter BN, Low EN, Nix DA, Yost CC, Zimmerman GA, Weyrich A (2011). Genome-wide RNA-seq analysis of human and mouse platelet transcriptomes. Blood, 118(14), e101-11.
  3. McInturff AM, Cody MJ, Elliott EA, Glenn JW, Rowley JW, Rondina MT, Yost C (2012). Mammalian target of rapamycin regulates neutrophil extracellular trap formation via induction of hypoxia-inducible factor 1 ¿. Blood, 120(15), 3118-25.
  4. Rowley JW, Chappaz S, Corduan A, Chong MM, Campbell R, Khoury A, Manne BK, Wurtzel JG, Michael JV, Goldfinger LE, Mumaw MM, Nieman MT, Kile BT, Provost P, Weyrich A (2016). Dicer1-mediated miRNA processing shapes the mRNA profile and function of murine platelets. Blood, 127(14), 1743-51.
  5. Michael JV, Wurtzel JGT, Mao GF, Rao AK, Kolpakov MA, Sabri A, Hoffman NE, Rajan S, Tomar D, Madesh M, Nieman MT, Yu J, Edelstein LC, Rowley JW, Weyrich AS, Goldfinger L (2017). Platelet microparticles infiltrating solid tumors transfer miRNAs that suppress tumor growth. Blood, 130(5), 567-580.
  6. Campbell RA, Schwertz H, Hottz ED, Rowley JW, Manne BK, Washington AV, Hunter-Mellado R, Tolley ND, Christensen M, Eustes AS, Montenont E, Bhatlekar S, Ventrone CH, Kirkpatrick BD, Pierce KK, Whitehead SS, Diehl SA, Bray PF, Zimmerman GA, Kosaka Y, Bozza PT, Bozza FA, Weyrich AS, Rondina M (2019). Human megakaryocytes possess intrinsic antiviral immunity through regulated induction of IFITM3. Blood, 133(19), 2013-2026.
  7. Morales-Ortíz J, Deal V, Reyes F, Maldonado-Martínez G, Ledesma N, Staback F, Croft C, Pacheco A, Ortiz-Zuazaga H, Yost CC, Rowley JW, Madera B, John AS, Chen J, Lopez J, Rondina MT, Hunter R, Gibson A, Washington A (2018). Platelet-derived TLT-1 is a prognostic indicator in ALI/ARDS and prevents tissue damage in the lungs in a mouse model. Blood, 132(23), 2495-2505.
  8. Middleton EA, Rowley JW, Campbell RA, Grissom CK, Brown SM, Beesley SJ, Schwertz H, Kosaka Y, Manne BK, Krauel K, Tolley ND, Eustes AS, Guo L, Paine R 3rd, Harris ES, Zimmerman GA, Weyrich AS, Rondina M (2019). Sepsis alters the transcriptional and translational landscape of human and murine platelets. Blood, 134(12), 911-923.
  9. Manne BK, Denorme F, Middleton EA, Portier I, Rowley JW, Stubben C, Petrey AC, Tolley ND, Guo L, Cody M, Weyrich AS, Yost CC, Rondina MT, Campbell R (2020). Platelet gene expression and function in patients with COVID-19. Blood, 136(11), 1317-1329.
  10. Melki I, Allaeys I, Tessandier N, Mailhot B, Cloutier N, Campbell RA, Rowley JW, Salem D, Zufferey A, Laroche A, Lévesque T, Patey N, Rauch J, Lood C, Droit A, McKenzie SE, Machlus KR, Rondina MT, Lacroix S, Fortin P, Boilard (2020). Fc'RIIA expression aggravates nephritis and increases platelet activation in systemic lupus erythematosus in mice. Blood,
  11. Bhatlekar S, Manne BK, Basak I, Edelstein LC, Tugolukova E, Stoller ML, Cody MJ, Morley SC, Nagalla S, Weyrich AS, Rowley JW, O'Connell RM, Rondina MT, Campbell RA, Bray P (2020). miR-125a-5p regulates megakaryocyte proplatelet formation via the actin-bundling protein L-plastin. Blood, 136(15), 1760-1772.
  12. Guo L, Shen S, Rowley JW, Tolley ND, Jia W, Manne BK, McComas KN, Bolingbroke B, Kosaka Y, Krauel K, Denorme F, Jacob SP, Eustes AS, Campbell RA, Middleton EA, He X, Brown SM, Morrell CN, Weyrich AS, Rondina M (2021). Platelet MHC Class I Mediates CD8+ T Cell Suppression During Sepsis. Blood, 138(5), 401-416.
  13. Melki I, Allaeys I, Tessandier N, Mailhot B, Cloutier N, Campbell RA, Rowley JW, Salem D, Zufferey A, Laroche A, Lévesque T, Patey N, Rauch J, Lood C, Droit A, McKenzie SE, Machlus KR, Rondina MT, Lacroix S, Fortin PR, Boilard (2020). Fc'RIIA expression accelerates nephritis and increases platelet activation in systemic lupus erythematosus. Blood, 136(25), 2933-2945.
  14. Kahr WH, Hinckley J, Li L, Schwertz H, Christensen H, Rowley JW, Pluthero FG, Urban D, Fabbro S, Nixon B, Gadzinski R, Storck M, Wang K, Ryu GY, Jobe SM, Schutte BC, Moseley J, Loughran NB, Parkinson J, Weyrich AS, Di Paola (2011). Mutations in NBEAL2, encoding a BEACH protein, cause gray platelet syndrome. Nature genetics, 43(8), 738-40.
  15. Noetzli L, Lo RW, Lee-Sherick AB, Callaghan M, Noris P, Savoia A, Rajpurkar M, Jones K, Gowan K, Balduini C, Pecci A, Gnan C, De Rocco D, Doubek M, Li L, Lu L, Leung R, Landolt-Marticorena C, Hunger S, Heller P, Gutierrez-Hartmann A, Xiayuan L, Pluthero FG, Rowley JW, Weyrich AS, Kahr WHA, Porter CC, Di Paola (2015). Germline mutations in ETV6 are associated with thrombocytopenia, red cell macrocytosis and predisposition to lymphoblastic leukemia. Nature genetics, 47(5), 535-538.
  16. Lin KY, Lu D, Hung CF, Peng S, Huang L, Jie C, Murillo F, Rowley J, Tsai YC, He L, Kim DJ, Jaffee E, Pardoll D, Wu T (2007). Ectopic expression of vascular cell adhesion molecule-1 as a new mechanism for tumor immune evasion. Cancer research, 67(4), 1832-41.
  17. Rondina MT, Voora D, Simon LM, Schwertz H, Harper JF, Lee O, Bhatlekar SC, Li Q, Eustes AS, Montenont E, Campbell RA, Tolley ND, Kosaka Y, Weyrich AS, Bray PF, Rowley J (2020). Longitudinal RNA-Seq Analysis of the Repeatability of Gene Expression and Splicing in Human Platelets Identifies a Platelet SELP Splice QTL. Circulation research, 126(4), 501-516.
  18. Rowley J, Monie A, Hung CF, Wu T (2009). Expression of IL-15RA or an IL-15/IL-15RA fusion on CD8+ T cells modifies adoptively transferred T-cell function in cis. European journal of immunology, 39(2), 491-506.
  19. Bhatlekar S, Basak I, Edelstein LC, Campbell RA, Lindsey CR, Italiano JE Jr, Weyrich AS, Rowley JW, Rondina MT, Sola-Visner M, Bray P (2019). Anti-apoptotic BCL2L2 increases megakaryocyte proplatelet formation in cultures of human cord blood. Haematologica, 104(10), 2075-2083.
  20. Sturrock A, Mir-Kasimov M, Baker J, Rowley J, Paine R 3r (2014). Key role of microRNA in the regulation of granulocyte macrophage colony-stimulating factor expression in murine alveolar epithelial cells during oxidative stress. The Journal of biological chemistry, 289(7), 4095-105.
  21. Thon JN, Peters CG, Machlus KR, Aslam R, Rowley J, Macleod H, Devine MT, Fuchs TA, Weyrich AS, Semple JW, Flaumenhaft R, Italiano JE J (2012). T granules in human platelets function in TLR9 organization and signaling. The Journal of cell biology, 198(4), 561-74.
  22. Shi DS, Smith MC, Campbell RA, Zimmerman PW, Franks ZB, Kraemer BF, Machlus KR, Ling J, Kamba P, Schwertz H, Rowley JW, Miles RR, Liu ZJ, Sola-Visner M, Italiano JE Jr, Christensen H, Kahr WH, Li DY, Weyrich A (2014). Proteasome function is required for platelet production. The Journal of clinical investigation, 124(9), 3757-66.
  23. Yost CC, Schwertz H, Cody MJ, Wallace JA, Campbell RA, Vieira-de-Abreu A, Araujo CV, Schubert S, Harris ES, Rowley JW, Rondina MT, Fulcher JM, Koening CL, Weyrich AS, Zimmerman G (2016). Neonatal NET-inhibitory factor and related peptides inhibit neutrophil extracellular trap formation. The Journal of clinical investigation, 126(10), 3783-3798.
  24. Rowley J, Monie A, Hung CF, Wu T (2008). Inhibition of tumor growth by NK1.1+ cells and CD8+ T cells activated by IL-15 through receptor beta/common gamma signaling in trans. Journal of immunology (Baltimore, Md., 181(12), 8237-47.
  25. Campbell RA, Franks Z, Bhatnagar A, Rowley JW, Manne BK, Supiano MA, Schwertz H, Weyrich AS, Rondina M (2018). Granzyme A in Human Platelets Regulates the Synthesis of Proinflammatory Cytokines by Monocytes in Aging. Journal of immunology (Baltimore, Md., 200(1), 295-304.
  26. Denis HL, Lamontagne-Proulx J, St-Amour I, Mason SL, Rowley JW, Cloutier N, Tremblay MÈ, Vincent AT, Gould PV, Chouinard S, Weyrich AS, Rondina MT, Barker RA, Boilard E, Cicchetti (2019). Platelet abnormalities in Huntington's disease. Journal of neurology, neurosurgery, and psychiatry, 90(3), 272-283.
  27. Fidler TP, Rowley JW, Araujo C, Boudreau LH, Marti A, Souvenir R, Dale K, Boilard E, Weyrich AS, Abel E (2017). Superoxide Dismutase 2 is dispensable for platelet function. Thrombosis and haemostasis, 117(10), 1859-1867.
  28. Fidler TP, Middleton EA, Rowley JW, Boudreau LH, Campbell RA, Souvenir R, Funari T, Tessandier N, Boilard E, Weyrich AS, Abel E (2017). Glucose Transporter 3 Potentiates Degranulation and Is Required for Platelet Activation. Arteriosclerosis, thrombosis, and vascular biology, 37(9), 1628-1639.
  29. Campbell RA, Vieira-de-Abreu A, Rowley JW, Franks ZG, Manne BK, Rondina MT, Kraiss LW, Majersik JJ, Zimmerman GA, Weyrich A (2017). Clots Are Potent Triggers of Inflammatory Cell Gene Expression: Indications for Timely Fibrinolysis. Arteriosclerosis, thrombosis, and vascular biology, 37(10), 1819-1827.
  30. Schwertz H, Rowley JW, Schumann GG, Thorack U, Campbell RA, Manne BK, Zimmerman GA, Weyrich AS, Rondina M (2018). Endogenous LINE-1 (Long Interspersed Nuclear Element-1) Reverse Transcriptase Activity in Platelets Controls Translational Events Through RNA-DNA Hybrids. Arteriosclerosis, thrombosis, and vascular biology, 38(4), 801-815.
  31. Rondina MT, Freitag M, Pluthero FG, Kahr WH, Rowley JW, Kraiss LW, Franks Z, Zimmerman GA, Weyrich AS, Schwertz (2016). Non-genomic activities of retinoic acid receptor alpha control actin cytoskeletal events in human platelets. Journal of thrombosis and haemostasis, 14(5), 1082-94.
  32. Nance D, Campbell RA, Rowley JW, Downie JM, Jorde LB, Kahr WH, Mereby SA, Tolley ND, Zimmerman GA, Weyrich AS, Rondina M (2016). Combined variants in factor VIII and prostaglandin synthase-1 amplify hemorrhage severity across three generations of descendants. Journal of thrombosis and haemostasis, 14(11), 2230-2240.
  33. Schwertz H, Rowley JW, Zimmerman GA, Weyrich AS, Rondina M (2017). Retinoic acid receptor-¿ regulates synthetic events in human platelets. Journal of thrombosis and haemostasis, 15(12), 2408-2418.
  34. Eustes AS, Campbell RA, Middleton EA, Tolley ND, Manne BK, Montenont E, Rowley JW, Krauel K, Blair A, Guo L, Kosaka Y, Medeiros-de-Moraes IM, Lacerda M, Hottz ED, Neto HCF, Zimmerman GA, Weyrich AS, Petrey A, Rondina M (2021). Heparanase expression and activity are increased in platelets during clinical sepsis. Journal of thrombosis and haemostasis, 19(5), 1319-1330.
  35. Rowley JW, Finn AV, French PA, Jennings LK, Bluestein D, Gross PL, Freedman JE, Steinhubl SR, Zimmerman GA, Becker RC, Dauerman HL, Smyth SS, 2011 Platelet Colloquium Participants (2012). Cardiovascular devices and platelet interactions: understanding the role of injury, flow, and cellular responses. Circulation. Cardiovascular interventions, 5(2), 296-304.
  36. Fisher MH, Kirkpatrick GD, Stevens B, Jones C, Callaghan M, Rajpurkar M, Fulbright J, Cooper MA, Rowley J, Porter CC, Gutierrez-Hartmann A, Jones K, Jordan C, Pietras EM, Di Paola (2020). ETV6 germline mutations cause HDAC3/NCOR2 mislocalization and upregulation of interferon response genes. JCI insight, 5(18),
  37. Montenont E, Bhatlekar S, Jacob S, Kosaka Y, Manne BK, Lee O, Parra-Izquierdo I, Tugolukova E, Tolley ND, Rondina MT, Bray PF, Rowley J (2021). CRISPR-edited megakaryocytes for rapid screening of platelet gene functions. Blood advances, 5(9), 2362-2374.
  38. Stoller ML, Basak I, Denorme F, Rowley JW, Alsobrooks J, Parsawar K, Nieman MT, Yost CC, Hamilton JR, Bray PF, Campbell R (2022). Neutrophil cathepsin G proteolysis of protease-activated receptor 4 generates a novel, functional tethered ligand. Blood advances, 6(7), 2303-2308.
  39. Bhatlekar S, Jacob S, Manne BK, Guo L, Denorme F, Tugolukova EA, Cody MJ, Kosaka Y, Rigoutsos I, Campbell RA, Rowley JW, O'Connell RM, Bray P (2021). Megakaryocyte-specific knockout of the Mir-99b/let7e/125a cluster lowers platelet count without altering platelet function. Blood cells, molecules & diseases, 92, 102624.
  40. Wurtzel JGT, Lazar S, Sikder S, Cai KQ, Astsaturov I, Weyrich AS, Rowley JW, Goldfinger L (2021). Platelet microRNAs inhibit primary tumor growth via broad modulation of tumor cell mRNA expression in ectopic pancreatic cancer in mice. PloS one, 16(12), e0261633.
  41. Schwertz H, Rowley JW, Portier I, Middleton EA, Tolley ND, Campbell RA, Eustes AS, Chen K, Rondina M (2021). Human platelets display dysregulated sepsis-associated autophagy, induced by altered LC3 protein-protein interaction of the Vici-protein EPG5. Autophagy, 18(7), 1-17.
  42. Shen Z, Du W, Perkins C, Fechter L, Natu V, Maecker H, Rowley J, Gotlib J, Zehnder J, Krishnan (2021). Platelet transcriptome identifies progressive markers and potential therapeutic targets in chronic myeloproliferative neoplasms. Cell reports. Medicine, 2(10), 100425.
  43. Manne BK, Campbell RA, Bhatlekar S, Ajanel A, Denorme F, Portier I, Middleton EA, Tolley ND, Kosaka Y, Montenont E, Guo L, Rowley JW, Bray PF, Jacob SP, Fukanaga R, Proud CG, Weyrich AS, Rondina M (2022). MAPK-interacting kinase 1 (Mnk1) regulates platelet production, activation, and thrombosis. Blood, 140(23), 2477-2489.
  44. Zhou C, Uluisik R, Rowley JW, David C, Jones CL, Scharer CD, Noetzli L, Fisher MH, Kirkpatrick GD, Bark K, Boss JM, Henry CJ, Pietras EM, Di Paola J, Porter C (2022). Germline ETV6 mutation promotes inflammation and disrupts lymphoid development of early hematopoietic progenitors. Experimental hematology, 112-113, 24-34.
  45. Grichine A, Jacob SP, Eckly AE, Villaret J, Joubert C, Appaix F, Pezet M, Ribba AS, Denarier E, Mazzega J, Rinckel JY, Lafanechère L, Elena-Herrmann B, Rowley JW, Sadoul (2023). The fate of mitochondria during platelet activation. Blood advances,
  46. Jacob S, Kosaka Y, Bhatlekar S, Denorme F, Benzon H, Moody A, Moody V, Tugolukova EA, Hull G, Kishimoto N, Manne BK, Guo L, Souvenir R, Seliger BJ, Eustes AS, Hoerger K, Tolley ND, Fatahian AN, Boudina S, Christiani DC, Wei Y, Ju C, Campbell RA, Rondina MT, Abel ED, Bray PF, Weyrich AS, Rowley J (2024). Mitofusin-2 Regulates Platelet Mitochondria and Function. Circulation research, 134(2), 143-161.
  47. Banerjee M, Rowley JW, Stubben CJ, Tolley ND, Freson K, Nelson B, Nagy B Jr, Fejes Z, Blair AM, Turro E, Gresele P, Taranta GC, Bury L, Falcinelli E, Lordkipanidzé M, Alessi MC, Johnson AD, Bakchoul T, Ramstrom S, Frontini M, Camera M, Brambilla M, Campbell RA, Rondina M (2024). Prospective, International, Multisite Comparison of Platelet Isolation Techniques for Genome-Wide Transcriptomics: Communication from the SSC of the ISTH. Journal of thrombosis and haemostasis, 22(10), 2922-2934.

Review

  1. Schubert S, Weyrich AS, Rowley J (2014). A tour through the transcriptional landscape of platelets. Blood, 124(4), 493-502.
  2. Davizon-Castillo P, Rowley JW, Rondina M (2020). Megakaryocyte and Platelet Transcriptomics for Discoveries in Human Health and Disease. Arteriosclerosis, thrombosis, and vascular biology, ATVBAHA119313280.
  3. Rowley JW, Schwertz H, Weyrich A (2012). Platelet mRNA: the meaning behind the message. Current opinion in hematology, 19(5), 385-91.

Book Chapter

  1. Schwertz H, Rowley JW, Tolley ND, Campbell RA, Weyrich A (2012). Assessing protein synthesis by platelets. Methods in molecular biology (Clifton, N.J.), 788, 141-53.
  2. Rowley JW,Weyrich AS,Bray P (2019). The platelet transcriptome in health and disease. Platelets, 139-153.
  3. Rowley JW,Manne BK,Weyrich A (2017). The platelet transcriptome: Coding RNAs. Platelets in Thrombotic and Non-Thrombotic Disorders: Pathophysiology, Pharmacology and Therapeutics: an Update, 227-238.

Commentary

  1. Rowley JW,Weyrich AS,Campbell R (2009). TGFBIp: More than meets the eye?. Blood, 114(25), 5113-5114.

Editorial

  1. Middleton EA, Rowley J (2021). eQTLs in platelets and iPSC-megakaryocytes. Blood, 137(7), 869-870.

Letter

  1. Rowley JW, Weyrich A (2013). Coordinate expression of transcripts and proteins in platelets. Blood, 121(26), 5255-6.

Abstract

  1. Shancy Jacob, Marina Tistao, Abigail Ajanel Gomez, Frederik Denorme, Yasuhiro Kosaka, Bhanu Kanth Manne, Li Guo, Robert A. Campbell, Matthew T. Rondina, Jesse W Rowle (2023). Mitochondrial Fission Protein Drp1 Regulates Platelet Function. 142(Supplement 1), 1201.