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John B. McCullough

John B. McCullough, PhD, BSc

Languages spoken: English

Academic Information

Departments Primary - Biochemistry

Academic Office Information

Research Interests

  • Cryogenic Electron Microscopy and Three-Dimensional Reconstruction (cryo-EM)
  • ESCRT Pathway Structure and Function

Education History

Postdoctoral Training University of Utah
Postdoctoral Training
University of Liverpool
Undergraduate National University of Ireland, Galway

Selected Publications

Journal Article

  1. Lara Rheinemann, Diane Miller Downhour, Kate Bredbenner, Gaelle Mercenne, Kristen A Davenport, Phuong Tieu Schmitt, Christina R Necessary, John mcCullough, Anthony P Schmitt, Sanford M Simon, Wesley I Sundquist, Nels C Elde (2021). RetroCHMP3 blocks budding of enveloped viruses without blocking cytokinesis. Cell, 184(21), 5419-5431.
  2. Nguyen HC, Talledge N, McCullough J, Sharma A, Moss FR 3rd, Iwasa JH, Vershinin MD, Sundquist WI, Frost A (2020). Membrane constriction and thinning by sequential ESCRT-III polymerization. Nat Struct Mol Biol, 27(4), 392-399.
  3. Han H, Schubert HL, McCullough J, Monroe N, Purdy MD, Yeager M, Sundquist WI, Hill CP (2019). Structure of spastin bound to a glutamate-rich peptide implies a hand-over-hand mechanism of substrate translocation. J Biol Chem, 295(2), 435-443.
  4. Romanov V, McCullough J, Gale BK, Frost A (2019). A tunable microfluidic device enables cargo encapsulation by cell-or organelle-sized lipid vesicles comprising asymmetric lipid bilayers  . Adv Biosyst, 2019(1900010),
  5. Talledge N, McCullough J, Wenzel D, Nguyen HC, Lalonde MS, Bajorek M, Skalicky J, Frost A, Sundquist WI (August 7, 2018). The ESCRT-III proteins IST1 and CHMP1B assemble around nucleic acids. BioRxiv.
  6. McCullough J, Clippinger AK, Talledge N, Skowyra ML, Saunders MG, Naismith TV, Colf LA, Afonine P, Arthur C, Sundquist WI, Hanson PI, Frost A (2015). Structure and membrane remodeling activity of ESCRT-III helical polymers. Science, 350(6267), 1548-51.
  7. McCullough J, Sundquist WI (2014). Putting a finger in the ring. Nat Struct Mol Biol, 21(12), 1025-7.
  8. Dobro MJ, Samson RY, Yu Z, McCullough J, Ding HJ, Chong PL, Bell SD, Jensen GJ (2013). Electron cryotomography of ESCRT assemblies and dividing Sulfolobus cells suggests that spiraling filaments are involved in membrane scission. Mol Biol Cell, 24(15), 2319-27.
  9. Morita E, Sandrin V, McCullough J, Katsuyama A, Baci Hamilton I, Sundquist WI (2011). ESCRT-III protein requirements for HIV-1 budding. Cell Host Microbe, 9(3), 235-42.
  10. Bajorek M, Schubert HL, McCullough J, Langelier C, Eckert DM, Stubblefield WM, Uter NT, Myszka DG, Hill CP, Sundquist WI (2009). Structural basis for ESCRT-III protein autoinhibition. Nat Struct Mol Biol, 16(7), 754-62.
  11. McCullough J, Fisher RD, Whitby FG, Sundquist WI, Hill CP (2008). ALIX-CHMP4 interactions in the human ESCRT pathway. Proc Natl Acad Sci U S A, 105(22), 7687-91.
  12. Urbe S, McCullough J, Row P, Prior IA, Welchman R, Clague MJ (2006). Control of growth factor receptor dynamics by reversible ubiquitination. Biochem Soc Trans, 34(Pt 5), 754-6.
  13. Row PE, Prior IA, McCullough J, Clague MJ, Urbe S (2006). The ubiquitin isopeptidase UBPY regulates endosomal ubiquitin dynamics and is essential for receptor down-regulation. J Biol Chem, 281(18), 12618-24.
  14. McCullough J, Row PE, Lorenzo O, Doherty M, Beynon R, Clague MJ, Urbe S (2006). Activation of the endosome-associated ubiquitin isopeptidase AMSH by STAM, a component of the multivesicular body-sorting machinery. Curr Biol, 16(2), 160-5.
  15. McCullough J, Clague MJ, Urbe S (2004). AMSH is an endosome-associated ubiquitin isopeptidase. J Cell Biol, 166(4), 487-92.
  16. Hammond DE, Carter S, McCullough J, Urbe S, Vande Woude G, Clague MJ (2003). Endosomal dynamics of Met determine signaling output. Mol Biol Cell, 14(4), 1346-54.


  1. McCullough J, Frost A, Sundquist WI (2018). Structures, Functions, and Dynamics of ESCRT-III/Vps4 Membrane Remodeling and Fission Complexes. [Review]. Annu Rev Cell Dev Biol, 34, 85-109.
  2. McCullough J, Colf LA, Sundquist WI (2013). Membrane fission reactions of the mammalian ESCRT pathway. [Review]. Annu Rev Biochem, 82, 663-92.

Book Chapter

  1. McCullough J, Clague MJ, Urb S (2007). Ubiquitin and protein sorting to the lysosome. In Mayer RJ, Ciechanover A, Rechsteiner M (Eds.), Protein Degradation: Cell Biology of the Ubiquitin-Proteasome System (Volume 3, pp. 76-102). Weinheim: Wiley-VCH.


  1. McCullough J, Sundquist WI (2020). Membrane Remodeling: ESCRT-III Filaments as Molecular Garrotes. Curr Biol, 30(23), R1425-R1428.

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