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Justin A. Bosch, PhD

Academic Information

Departments Primary - Human Genetics

Education History

Undergraduate University of Wisconsin¿Madison
 BS
Graduate Training University of California, Berkeley
 PhD
Postdoctoral Fellowship Harvard Medical School
 Postdoctoral Fellow

Selected Publications

Journal Article

  1. Bosch JA, Keith N, Escobedo F, Fisher WW, LaGraff JT, Rabasco J, Wan KH, Weiszmann R, Hu Y, Kondo S, Brown JB, Perrimon N, Celniker SE (2023). Molecular and functional characterization of the Drosophila melanogaster conserved smORFome. Cell Rep, 42(11), 113311.
  2. Bosch JA, Ugur B, Pichardo-Casas I, Rabasco J, Escobedo F, Zuo Z, Brown B, Celniker S, Sinclair DA, Bellen HJ, Perrimon N (2022). Two neuronal peptides encoded from a single transcript regulate mitochondrial complex III in Drosophila. Elife, 11.
  3. Bosch JA, Perrimon N (2022). Prime Editing for Precise Genome Engineering in Drosophila. Methods Mol Biol, 2540, 113-134.
  4. Droujinine IA, Meyer AS, Wang D, Udeshi ND, Hu Y, Rocco D, McMahon JA, Yang R, Guo J, Mu L, Carey DK, Svinkina T, Zeng R, Branon T, Tabatabai A, Bosch JA, Asara JM, Ting AY, Carr SA, McMahon AP, Perrimon N (2021). Proteomics of protein trafficking by in vivo tissue-specific labeling. Nat Commun, 12(1), 2382.
  5. Bosch JA, Birchak G, Perrimon N (2021). Precise genome engineering in Drosophila using prime editing. Proc Natl Acad Sci U S A, 118(1).
  6. Xu C, Franklin B, Tang HW, Regimbald-Dumas Y, Hu Y, Ramos J, Bosch JA, Villalta C, He X, Perrimon N (2020). An in vivo RNAi screen uncovers the role of AdoR signaling and adenosine deaminase in controlling intestinal stem cell activity. Proc Natl Acad Sci U S A, 117(1), 464-471.
  7. Bosch JA, Knight S, Kanca O, Zirin J, Yang-Zhou D, Hu Y, Rodiger J, Amador G, Bellen HJ, Perrimon N, Mohr SE (2020). Use of the CRISPR-Cas9 System in Drosophila Cultured Cells to Introduce Fluorescent Tags into Endogenous Genes. Curr Protoc Mol Biol, 130(1), e112.
  8. Bosch JA, Colbeth R, Zirin J, Perrimon N (2020). Gene Knock-Ins in Drosophila Using Homology-Independent Insertion of Universal Donor Plasmids. Genetics, 214(1), 75-89.
  9. Bosch JA, Tran NH, Hariharan IK (2015). CoinFLP: a system for efficient mosaic screening and for visualizing clonal boundaries in Drosophila. Development, 142(3), 597-606.
  10. Bosch JA, Sumabat TM, Hafezi Y, Pellock BJ, Gandhi KD, Hariharan IK (2014). The Drosophila F-box protein Fbxl7 binds to the protocadherin fat and regulates Dachs localization and Hippo signaling. Elife, 3, e03383.
  11. Anderson RM, Delous M, Bosch JA, Ye L, Robertson MA, Hesselson D, Stainier DY (2013). Hepatocyte growth factor signaling in intrapancreatic ductal cells drives pancreatic morphogenesis. PLoS Genet, 9(7), e1003650.
  12. Hafezi Y, Bosch JA, Hariharan IK (2012). Differences in levels of the transmembrane protein Crumbs can influence cell survival at clonal boundaries. Dev Biol, 368(2), 358-69.
  13. Zirin J, Jusiak B, Lopes R, Ewen-Campen B, Bosch J, Risbeck A, Forman C, Villalta C, Hu Y, Perrimon N (2023). Expanding the Drosophila toolkit for dual control of gene expression. bioRxiv.
  14. Branon TC, Bosch JA, Sanchez AD, Udeshi ND, Svinkina T, Carr SA, Feldman JL, Perrimon N, Ting AY (2018). Efficient proximity labeling in living cells and organisms with TurboID. Nat Biotechnol, 36(9), 880-887.
  15. Bosch JA, Sumabat TM, Hariharan IK (2016). Persistence of RNAi-Mediated Knockdown in Drosophila Complicates Mosaic Analysis Yet Enables Highly Sensitive Lineage Tracing. Genetics, 203(1), 109-18.
  16. Tittle RK, Sze R, Ng A, Nuckels RJ, Swartz ME, Anderson RM, Bosch J, Stainier DY, Eberhart JK, Gross JM (2011). Uhrf1 and Dnmt1 are required for development and maintenance of the zebrafish lens. Dev Biol, 350(1), 50-63.
  17. Anderson RM, Bosch JA, Goll MG, Hesselson D, Dong PD, Shin D, Chi NC, Shin CH, Schlegel A, Halpern M, Stainier D (2009). Loss of Dnmt1 catalytic activity reveals multiple roles for DNA methylation during pancreas development and regeneration. Developmental biology, 334(1), 213-23.
  18. Bosch JA, Heo K, Sliwinski MK, Baum D (2008). An exploration of LEAFY expression in independent evolutionary origins of rosette flowering in Brassicaceae. American journal of botany, 95(3), 286-93.
  19. Sliwinski MK, Bosch JA, Yoon HS, Balthazar Mv, Baum D (2007). The role of two LEAFY paralogs from Idahoa scapigera (Brassicaceae) in the evolution of a derived plant architecture. The Plant journal, 51(2), 211-9.
  20. McGee AV, Liu YV, Griffith AL, Szegletes ZM, Wen B, Kraus C, Miller NW, Steger RJ, Velasco BE, Bosch JA, Zirin JD, Viswanatha R, Sontheimer EJ, Goodale A, Greene MA, Green TM, Doench J (2023). Modular vector assembly enables rapid assessment of emerging CRISPR technologies. bioRxiv,

Review

  1. Bosch JA, Chen CL, Perrimon N (2021). Proximity-dependent labeling methods for proteomic profiling in living cells: An update. [Review]. Wiley Interdiscip Rev Dev Biol, 10, (1), e392.
  2. Zirin J, Bosch J, Viswanatha R, Mohr SE, Perrimon N (2022). State-of-the-art CRISPR for in vivo and cell-based studies in Drosophila. [Review]. Trends Genet, 38, (5), 437-453.