Barbie K. Ganser-Pornillos

Barbie K. Ganser-Pornillos, PhD

Academic Information

Departments Primary - Biochemistry

Academic Office Information

barbie.pornillos@cores.utah.edu

Research Interests

  • HIV Structure
  • CryoEM/CryoET
  • Retroviral Restriction by the Innate Immune System

Education History

Postdoctoral Fellowship The Scripps Research Institute
 Postdoctoral Fellow
University of Utah
 PhD
Undergraduate Montana Tech of the University of Montana
 BS

Selected Publications

Journal Article

  1. Sarkar S, Zadrozny KK, Zadorozhnyi R, Russell RW, Quinn CM, Kleinpeter A, Ablan S, Meshkin H, Perilla JR, Freed EO, Ganser-Pornillos BK, Pornillos O, Gronenborn AM, Polenova T (2023). Structural basis of HIV-1 maturation inhibitor binding and activity. Nat Commun, 14(1), 1237.
  2. Schirra RT, Dos Santos NFB, Zadrozny KK, Kucharska I, Ganser-Pornillos BK, Pornillos O (2023). A molecular switch modulates assembly and host factor binding of the HIV-1 capsid. Nat Struct Mol Biol, 30(3), 383-390.
  3. Odongo L, Zadrozny KK, Diehl WE, Luban J, White JM, Ganser-Pornillos BK, Tamm LK, Pornillos O (2023). Purification and structure of luminal domain C of human Niemann-Pick C1 protein. Acta Crystallogr F Struct Biol Commun, 79(Pt 2), 45-50.
  4. Yu A, Lee EMY, Briggs JAG, Ganser-Pornillos BK, Pornillos O, Voth GA (2022). Strain and rupture of HIV-1 capsids during uncoating. Proc Natl Acad Sci U S A, 119(10), e2117781119.
  5. Xue G, Braczyk K, Gonalves-Carneiro D, Dawidziak DM, Sanchez K, Ong H, Wan Y, Zadrozny KK, Ganser-Pornillos BK, Bieniasz PD, Pornillos O (2022). Poly(ADP-ribose) potentiates ZAP antiviral activity. PLoS Pathog, 18(2), e1009202.
  6. Zurowska K, Alam A, Ganser-Pornillos BK, Pornillos O (2021). Structural evidence that MOAP1 and PEG10 are derived from retrovirus/retrotransposon Gag proteins. Proteins, 90(1), 309-313.
  7. Zadorozhnyi R, Sarkar S, Quinn CM, Zadrozny KK, Ganser-Pornillos BK, Pornillos O, Gronenborn AM, Polenova T (2021). Determination of Histidine Protonation States in Proteins by Fast Magic Angle Spinning NMR. Front Mol Biosci, 8, 767040.
  8. Buckmaster MV, Zadrozny KK, Ganser-Pornillos BK, Pornillos O, Goff SP (2021). Structural Determinants of Virion Assembly and Release in the C Terminus of the Mason-Pfizer Monkey Virus Capsid Protein. J Virol, 95(19), e0061521.
  9. Ward AE, Kiessling V, Pornillos O, White JM, Ganser-Pornillos BK, Tamm LK (2020). HIV-cell membrane fusion intermediates are restricted by Serincs as revealed by cryo-electron and TIRF microscopy. J Biol Chem, 295(45), 15183-15195.
  10. Christensen DE, Ganser-Pornillos BK, Johnson JS, Pornillos O, Sundquist WI (2020). Reconstitution and visualization of HIV-1 capsid-dependent replication and integration in vitro. Science, 370(6513).
  11. Yu A, Skorupka KA, Pak AJ, Ganser-Pornillos BK, Pornillos O, Voth GA (2020). TRIM5α self-assembly and compartmentalization of the HIV-1 viral capsid. Nat Commun, 11(1), 1307.
  12. Kucharska I, Ding P, Zadrozny KK, Dick RA, Summers MF, Ganser-Pornillos BK, Pornillos O (2020). Biochemical Reconstitution of HIV-1 Assembly and Maturation. J Virol, 94(5).
  13. Skorupka KA, Roganowicz MD, Christensen DE, Wan Y, Pornillos O, Ganser-Pornillos BK (2019). Hierarchical assembly governs TRIM5α recognition of HIV-1 and retroviral capsids. Sci Adv, 5(11), eaaw3631.
  14. Buffone C, Martinez-Lopez A, Fricke T, Opp S, Severgnini M, Cifola I, Petiti L, Frabetti S, Skorupka K, Zadrozny KK, Ganser-Pornillos BK, Pornillos O, Di Nunzio F, Diaz-Griffero F (2018). Nup153 Unlocks the Nuclear Pore Complex for HIV-1 Nuclear Translocation in Nondividing Cells. J Virol, 92(19).
  15. Dick RA, Zadrozny KK, Xu C, Schur FKM, Lyddon TD, Ricana CL, Wagner JM, Perilla JR, Ganser-Pornillos BK, Johnson MC, Pornillos O, Vogt VM (2018). Inositol phosphates are assembly co-factors for HIV-1. Nature, 560(7719), 509-512.
  16. Wagner JM, Christensen DE, Bhattacharya A, Dawidziak DM, Roganowicz MD, Wan Y, Pumroy RA, Demeler B, Ivanov DN, Ganser-Pornillos BK, Sundquist WI, Pornillos O (2018). General Model for Retroviral Capsid Pattern Recognition by TRIM5 Proteins. J Virol, 92(4).
  17. Roganowicz MD, Komurlu S, Mukherjee S, Plewka J, Alam SL, Skorupka KA, Wan Y, Dawidowski D, Cafiso DS, Ganser-Pornillos BK, Campbell EM, Pornillos O (2017). TRIM5α SPRY/coiled-coil interactions optimize avid retroviral capsid recognition. PLoS Pathog, 13(10), e1006686.
  18. Dawidziak DM, Sanchez JG, Wagner JM, Ganser-Pornillos BK, Pornillos O (2017). Structure and catalytic activation of the TRIM23 RING E3 ubiquitin ligase. Proteins, 85(10), 1957-1961.
  19. Yang ST, Kreutzberger AJB, Kiessling V, Ganser-Pornillos BK, White JM, Tamm LK (2017). HIV virions sense plasma membrane heterogeneity for cell entry. Sci Adv, 3(6), e1700338.
  20. Bayro MJ, Ganser-Pornillos BK, Zadrozny KK, Yeager M, Tycko R (2016). Helical Conformation in the CA-SP1 Junction of the Immature HIV-1 Lattice Determined from Solid-State NMR of Virus-like Particles. J Am Chem Soc, 138(37), 12029-32.
  21. Wagner JM, Zadrozny KK, Chrustowicz J, Purdy MD, Yeager M, Ganser-Pornillos BK, Pornillos O (2016). Crystal structure of an HIV assembly and maturation switch. Elife, 5.
  22. Wagner JM, Roganowicz MD, Skorupka K, Alam SL, Christensen D, Doss G, Wan Y, Frank GA, Ganser-Pornillos BK, Sundquist WI, Pornillos O (2016). Mechanism of B-box 2 domain-mediated higher-order assembly of the retroviral restriction factor TRIM5α. Elife, 5.
  23. Li YL, Chandrasekaran V, Carter SD, Woodward CL, Christensen DE, Dryden KA, Pornillos O, Yeager M, Ganser-Pornillos BK, Jensen GJ, Sundquist WI (2016). Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids. Elife, 5.
  24. Grime JMA, Dama JF, Ganser-Pornillos BK, Woodward CL, Jensen GJ, Yeager M, Voth GA (2016). Coarse-grained simulation reveals key features of HIV-1 capsid self-assembly. Nat Commun, 7, 11568.
  25. Bhattacharya A, Alam SL, Fricke T, Zadrozny K, Sedzicki J, Taylor AB, Demeler B, Pornillos O, Ganser-Pornillos BK, Diaz-Griffero F, Ivanov DN, Yeager M (2014). Structural basis of HIV-1 capsid recognition by PF74 and CPSF6. Proc Natl Acad Sci U S A, 111(52), 18625-30.
  26. Yeager M, Dryden KA, Ganser-Pornillos BK (2012). Lipid monolayer and sparse matrix screening for growing two-dimensional crystals for electron crystallography: methods and examples. Methods Mol Biol, 955, 527-37.
  27. Pornillos O, Ganser-Pornillos BK, Yeager M (2010). Atomic-level modelling of the HIV capsid. Nature, 469(7330), 424-7.
  28. Ganser-Pornillos BK, Chandrasekaran V, Pornillos O, Sodroski JG, Sundquist WI, Yeager M (2010). Hexagonal assembly of a restricting TRIM5alpha protein. Proc Natl Acad Sci U S A, 108(2), 534-9.
  29. Pornillos O, Ganser-Pornillos BK, Banumathi S, Hua Y, Yeager M (2010). Disulfide bond stabilization of the hexameric capsomer of human immunodeficiency virus. J Mol Biol, 401(5), 985-95.
  30. Pornillos O, Ganser-Pornillos BK, Kelly BN, Hua Y, Whitby FG, Stout CD, Sundquist WI, Hill CP, Yeager M (2009). X-ray structures of the hexameric building block of the HIV capsid. Cell, 137(7), 1282-92.
  31. Hanson MA, Brooun A, Baker KA, Jaakola VP, Roth C, Chien EY, Alexandrov A, Velasquez J, Davis L, Griffith M, Moy K, Ganser-Pornillos BK, Hua Y, Kuhn P, Ellis S, Yeager M, Stevens RC (2007). Profiling of membrane protein variants in a baculovirus system by coupling cell-surface detection with small-scale parallel expression. Protein Expr Purif, 56(1), 85-92.
  32. Ganser-Pornillos BK, Cheng A, Yeager M (2007). Structure of full-length HIV-1 CA: a model for the mature capsid lattice. Cell, 131(1), 70-9.
  33. Benjamin J, Ganser-Pornillos BK, Tivol WF, Sundquist WI, Jensen GJ (2004). Three-dimensional structure of HIV-1 virus-like particles by electron cryotomography. J Mol Biol, 346(2), 577-88.
  34. Ganser-Pornillos BK, von Schwedler UK, Stray KM, Aiken C, Sundquist WI (2004). Assembly properties of the human immunodeficiency virus type 1 CA protein. J Virol, 78(5), 2545-52.
  35. Ganser BK, Cheng A, Sundquist WI, Yeager M (2003). Three-dimensional structure of the M-MuLV CA protein on a lipid monolayer: a general model for retroviral capsid assembly. EMBO J, 22(12), 2886-92.
  36. Stierle DB, Stierle AA, Ganser BK (1999). Isolation of two highly methylated polyketide derivatives from a yew-associated penicillium species. J Nat Prod, 62(8), 1147-50.
  37. Ganser BK, Li S, Klishko VY, Finch JT, Sundquist WI (1999). Assembly and analysis of conical models for the HIV-1 core. Science, 283(5398), 80-3.
  38. Stierle DB, Stierle AA, Ganser B (1997). New phomopsolides from a Penicillium sp. J Nat Prod, 60(11), 1207-9.

Review

  1. Ganser-Pornillos BK, Pornillos O (2019). Restriction of HIV-1 and other retroviruses by TRIM5. [Review]. Nat Rev Microbiol, 17(9), 546-556.
  2. Pornillos O, Ganser-Pornillos BK (2019). Maturation of retroviruses. [Review]. Curr Opin Virol, 36, 47-55.
  3. Ganser-Pornillos BK, Yeager M, Pornillos O (2012). Assembly and architecture of HIV. [Review]. Adv Exp Med Biol, 726, 441-65.
  4. Ganser-Pornillos BK, Yeager M, Sundquist WI (2008). The structural biology of HIV assembly. [Review]. Curr Opin Struct Biol, 18(2), 203-17.