Owen Pornillos

Owen Pornillos, PhD

Languages spoken: Tagalog, English, Bikol

Academic Information

Departments Primary - Biochemistry

Academic Office Information

owen@biochem.utah.edu

Lab

Education History

Undergraduate University of the Philippines
 BS
Graduate Training University of Utah
 PhD
Postdoctoral Fellowship The Scripps Research Institute
 Postdoctoral Fellow

Selected Publications

Journal Article

  1. Yu A, Lee EMY, Briggs JAG, Ganser-Pornillos BK, Pornillos O, Voth G (2022). Strain and rupture of HIV-1 capsids during uncoating. Proceedings of the National Academy of Sciences of the United States of America, 119(10), e2117781119.
  2. Xue G, Braczyk K, Gonçalves-Carneiro D, Dawidziak DM, Sanchez K, Ong H, Wan Y, Zadrozny KK, Ganser-Pornillos BK, Bieniasz PD, Pornillos (2022). Poly(ADP-ribose) potentiates ZAP antiviral activity. PLoS pathogens, 18(2), e1009202.
  3. Zadorozhnyi R, Sarkar S, Quinn CM, Zadrozny KK, Ganser-Pornillos BK, Pornillos O, Gronenborn AM, Polenova (2021). Determination of Histidine Protonation States in Proteins by Fast Magic Angle Spinning NMR. Frontiers in molecular biosciences, 8, 767040.
  4. Zurowska K, Alam A, Ganser-Pornillos BK, Pornillos (2022). Structural evidence that MOAP1 and PEG10 are derived from retrovirus/retrotransposon Gag proteins. Proteins, 90(1), 309-313.
  5. Buckmaster MV, Zadrozny KK, Ganser-Pornillos BK, Pornillos O, Goff S (2021). Structural Determinants of Virion Assembly and Release in the C Terminus of the Mason-Pfizer Monkey Virus Capsid Protein. Journal of virology, 95(19), e0061521.
  6. Eschbach JE, Elliott JL, Li W, Zadrozny KK, Davis K, Mohammed SJ, Lawson DQ, Pornillos O, Engelman AN, Kutluay S (2020). Capsid Lattice Destabilization Leads to Premature Loss of the Viral Genome and Integrase Enzyme during HIV-1 Infection. Journal of virology, 95(2),
  7. Christensen DE, Ganser-Pornillos BK, Johnson JS, Pornillos O, Sundquist W (2020). Reconstitution and visualization of HIV-1 capsid-dependent replication and integration in vitro. Science (New York, N.Y.), 370(6513),
  8. Ward AE, Kiessling V, Pornillos O, White JM, Ganser-Pornillos BK, Tamm L (2020). HIV-cell membrane fusion intermediates are restricted by Serincs as revealed by cryo-electron and TIRF microscopy. The Journal of biological chemistry, 295(45), 15183-15195.
  9. Yu A, Skorupka KA, Pak AJ, Ganser-Pornillos BK, Pornillos O, Voth G (2020). TRIM5¿ self-assembly and compartmentalization of the HIV-1 viral capsid. Nature communications, 11(1), 1307.
  10. Kucharska I, Ding P, Zadrozny KK, Dick RA, Summers MF, Ganser-Pornillos BK, Pornillos (2020). Biochemical Reconstitution of HIV-1 Assembly and Maturation. Journal of virology, 94(5),
  11. Skorupka KA, Roganowicz MD, Christensen DE, Wan Y, Pornillos O, Ganser-Pornillos B (2019). Hierarchical assembly governs TRIM5¿ recognition of HIV-1 and retroviral capsids. Science advances, 5(11), eaaw3631.
  12. Kueck T, Bloyet LM, Cassella E, Zang T, Schmidt F, Brusic V, Tekes G, Pornillos O, Whelan SPJ, Bieniasz P (2019). Vesicular Stomatitis Virus Transcription Is Inhibited by TRIM69 in the Interferon-Induced Antiviral State. Journal of virology, 93(24),
  13. Stevens M, Franke B, Skorupka KA, Cafiso DS, Pornillos O, Mayans O, Norman D (2019). Exploration of the TRIM Fold of MuRF1 Using EPR Reveals a Canonical Antiparallel Structure and Extended COS-Box. Journal of molecular biology, 431(15), 2900-2909.
  14. Sundquist WI, Pornillos (2018). Retrovirus Restriction by TRIM5¿: RINGside at a Cage Fight. Cell host & microbe, 24(6), 751-753.
  15. Sanchez JG, Sparrer KMJ, Chiang C, Reis RA, Chiang JJ, Zurenski MA, Wan Y, Gack MU, Pornillos (2018). TRIM25 Binds RNA to Modulate Cellular Anti-viral Defense. Journal of molecular biology, 430(24), 5280-5293.
  16. Dick RA, Zadrozny KK, Xu C, Schur FKM, Lyddon TD, Ricana CL, Wagner JM, Perilla JR, Ganser-Pornillos BK, Johnson MC, Pornillos O, Vogt V (2018). Inositol phosphates are assembly co-factors for HIV-1. Nature, 560(7719), 509-512.
  17. Buffone C, Martinez-Lopez A, Fricke T, Opp S, Severgnini M, Cifola I, Petiti L, Frabetti S, Skorupka K, Zadrozny KK, Ganser-Pornillos BK, Pornillos O, Di Nunzio F, Diaz-Griffero (2018). Nup153 Unlocks the Nuclear Pore Complex for HIV-1 Nuclear Translocation in Nondividing Cells. Journal of virology, 92(19),
  18. Wagner JM, Christensen DE, Bhattacharya A, Dawidziak DM, Roganowicz MD, Wan Y, Pumroy RA, Demeler B, Ivanov DN, Ganser-Pornillos BK, Sundquist WI, Pornillos (2018). General Model for Retroviral Capsid Pattern Recognition by TRIM5 Proteins. Journal of virology, 92(4),
  19. Roganowicz MD, Komurlu S, Mukherjee S, Plewka J, Alam SL, Skorupka KA, Wan Y, Dawidowski D, Cafiso DS, Ganser-Pornillos BK, Campbell EM, Pornillos (2017). TRIM5¿ SPRY/coiled-coil interactions optimize avid retroviral capsid recognition. PLoS pathogens, 13(10), e1006686.
  20. Dawidziak DM, Sanchez JG, Wagner JM, Ganser-Pornillos BK, Pornillos (2017). Structure and catalytic activation of the TRIM23 RING E3 ubiquitin ligase. Proteins, 85(10), 1957-1961.
  21. Janczyk P¿, Skorupka KA, Tooley JG, Matson DR, Kestner CA, West T, Pornillos O, Stukenberg P (2017). Mechanism of Ska Recruitment by Ndc80 Complexes to Kinetochores. Developmental cell, 41(4), 438-449.e4.
  22. Wagner JM, Zadrozny KK, Chrustowicz J, Purdy MD, Yeager M, Ganser-Pornillos BK, Pornillos (2016). Crystal structure of an HIV assembly and maturation switch. eLife, 5,
  23. Sanchez JG, Chiang JJ, Sparrer KMJ, Alam SL, Chi M, Roganowicz MD, Sankaran B, Gack MU, Pornillos (2016). Mechanism of TRIM25 Catalytic Activation in the Antiviral RIG-I Pathway. Cell reports, 16(5), 1315-1325.
  24. Wagner JM, Roganowicz MD, Skorupka K, Alam SL, Christensen D, Doss G, Wan Y, Frank GA, Ganser-Pornillos BK, Sundquist WI, Pornillos (2016). Mechanism of B-box 2 domain-mediated higher-order assembly of the retroviral restriction factor TRIM5¿. eLife, 5,
  25. Li YL, Chandrasekaran V, Carter SD, Woodward CL, Christensen DE, Dryden KA, Pornillos O, Yeager M, Ganser-Pornillos BK, Jensen GJ, Sundquist W (2016). Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids. eLife, 5,
  26. Gres AT, Kirby KA, KewalRamani VN, Tanner JJ, Pornillos O, Sarafianos S (2015). STRUCTURAL VIROLOGY. X-ray crystal structures of native HIV-1 capsid protein reveal conformational variability. Science (New York, N.Y.), 349(6243), 99-103.
  27. Bhattacharya A, Alam SL, Fricke T, Zadrozny K, Sedzicki J, Taylor AB, Demeler B, Pornillos O, Ganser-Pornillos BK, Diaz-Griffero F, Ivanov DN, Yeager (2014). Structural basis of HIV-1 capsid recognition by PF74 and CPSF6. Proceedings of the National Academy of Sciences of the United States of America, 111(52), 18625-30.
  28. Sanchez JG, Okreglicka K, Chandrasekaran V, Welker JM, Sundquist WI, Pornillos (2014). The tripartite motif coiled-coil is an elongated antiparallel hairpin dimer. Proceedings of the National Academy of Sciences of the United States of America, 111(7), 2494-9.
  29. Pornillos O, Ganser-Pornillos BK, Yeager (2011). Atomic-level modelling of the HIV capsid. Nature, 469(7330), 424-7.
  30. Ganser-Pornillos BK, Chandrasekaran V, Pornillos O, Sodroski JG, Sundquist WI, Yeager (2011). Hexagonal assembly of a restricting TRIM5alpha protein. Proceedings of the National Academy of Sciences of the United States of America, 108(2), 534-9.
  31. Pornillos O, Ganser-Pornillos BK, Banumathi S, Hua Y, Yeager (2010). Disulfide bond stabilization of the hexameric capsomer of human immunodeficiency virus. Journal of molecular biology, 401(5), 985-95.
  32. Zhu D, Hensel J, Hilgraf R, Abbasian M, Pornillos O, Deyanat-Yazdi G, Hua XH, Cox (2010). Inhibition of protein kinase CK2 expression and activity blocks tumor cell growth. Molecular and cellular biochemistry, 333(1-2), 159-67.
  33. Pornillos O, Ganser-Pornillos BK, Kelly BN, Hua Y, Whitby FG, Stout CD, Sundquist WI, Hill CP, Yeager (2009). X-ray structures of the hexameric building block of the HIV capsid. Cell, 137(7), 1282-92.
  34. Chen YJ, Pornillos O, Lieu S, Ma C, Chen AP, Chang (2007). X-ray structure of EmrE supports dual topology model. Proceedings of the National Academy of Sciences of the United States of America, 104(48), 18999-9004.
  35. Zhang Z, Ma C, Pornillos O, Xiu X, Chang G, Saier MH J (2007). Functional characterization of the heterooligomeric EbrAB multidrug efflux transporter of Bacillus subtilis. Biochemistry, 46(17), 5218-25.
  36. Pornillos O, Chen YJ, Chen AP, Chang (2005). X-ray structure of the EmrE multidrug transporter in complex with a substrate. Science (New York, N.Y.), 310(5756), 1950-3.
  37. Pornillos O, Higginson DS, Stray KM, Fisher RD, Garrus JE, Payne M, He GP, Wang HE, Morham SG, Sundquist W (2003). HIV Gag mimics the Tsg101-recruiting activity of the human Hrs protein. The Journal of cell biology, 162(3), 425-34.
  38. Pornillos O, Alam SL, Davis DR, Sundquist W (2002). Structure of the Tsg101 UEV domain in complex with the PTAP motif of the HIV-1 p6 protein. Nature structural biology, 9(11), 812-7.
  39. Pornillos O, Alam SL, Rich RL, Myszka DG, Davis DR, Sundquist W (2002). Structure and functional interactions of the Tsg101 UEV domain. The EMBO journal, 21(10), 2397-406.
  40. Garrus JE, von Schwedler UK, Pornillos O, Morham SG, Zavitz KH, Wang HE, Wettstein DA, Stray KM, Côté M, Rich RL, Myszka DG, Sundquist W (2001). Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding. Cell, 107(1), 55-65.
  41. Jenkins Y, Pornillos O, Rich RL, Myszka DG, Sundquist WI, Malim M (2001). Biochemical analyses of the interactions between human immunodeficiency virus type 1 Vpr and p6(Gag). Journal of virology, 75(21), 10537-42.
  42. Sparrer KMJ, Gableske S, Zurenski MA, Parker ZM, Full F, Baumgart GJ, Kato J, Pacheco-Rodriguez G, Liang C, Pornillos O, Moss J, Vaughan M, Gack M (2017). TRIM23 mediates virus-induced autophagy via activation of TBK1. Nature microbiology, 2(11), 1543-1557.

Review

  1. Ganser-Pornillos BK, Pornillos (2019). Restriction of HIV-1 and other retroviruses by TRIM5. Nature reviews. Microbiology, 17(9), 546-556.
  2. Pornillos O, Ganser-Pornillos B (2019). Maturation of retroviruses. Current opinion in virology, 36, 47-55.
  3. Ganser-Pornillos BK, Yeager M, Pornillos (2012). Assembly and architecture of HIV. Advances in experimental medicine and biology, 726, 441-65.
  4. Pornillos O, Chang (2006). Inverted repeat domains in membrane proteins. FEBS letters, 580(2), 358-62.
  5. Pornillos O, Garrus JE, Sundquist W (2002). Mechanisms of enveloped RNA virus budding. Trends in cell biology, 12(12), 569-79.