< content="width=device-width, initial-scale=1.0"> Owen Pornillos | School of Medicine
Owen Pornillos

Owen Pornillos, PhD

Languages spoken: Bikol, English, Tagalog

Academic Information

Departments Primary - Biochemistry

Lab

Research Interests

  • HIV Structure
  • Structural Biology of the Innate Immune System
  • Virus/Host Interactions
  • X-Ray Crystallography and Cryo-EM

Education History

Postdoctoral Fellowship The Scripps Research Institute
 Postdoctoral Fellow
University of Utah
 PhD
Undergraduate University of the Philippines
 BS

Selected Publications

Journal Article

  1. Yu A, Lee EMY, Briggs JAG, Ganser-Pornillos BK, Pornillos O, Voth GA (2022). Strain and rupture of HIV-1 capsids during uncoating. Proc Natl Acad Sci U S A, 119(10), e2117781119. (Read full article)
  2. Xue G, Braczyk K, Gonalves-Carneiro D, Dawidziak DM, Sanchez K, Ong H, Wan Y, Zadrozny KK, Ganser-Pornillos BK, Bieniasz PD, Pornillos O (2022). Poly(ADP-ribose) potentiates ZAP antiviral activity. PLoS Pathog, 18(2), e1009202. (Read full article)
  3. Zurowska K, Alam A, Ganser-Pornillos BK, Pornillos O (2021). Structural evidence that MOAP1 and PEG10 are derived from retrovirus/retrotransposon Gag proteins. Proteins, 90(1), 309-313. (Read full article)
  4. Zadorozhnyi R, Sarkar S, Quinn CM, Zadrozny KK, Ganser-Pornillos BK, Pornillos O, Gronenborn AM, Polenova T (2021). Determination of Histidine Protonation States in Proteins by Fast Magic Angle Spinning NMR. Front Mol Biosci, 8, 767040. (Read full article)
  5. Buckmaster MV, Zadrozny KK, Ganser-Pornillos BK, Pornillos O, Goff SP (2021). Structural Determinants of Virion Assembly and Release in the C Terminus of the Mason-Pfizer Monkey Virus Capsid Protein. J Virol, 95(19), e0061521. (Read full article)
  6. Eschbach JE, Elliott JL, Li W, Zadrozny KK, Davis K, Mohammed SJ, Lawson DQ, Pornillos O, Engelman AN, Kutluay SB (2020). Capsid Lattice Destabilization Leads to Premature Loss of the Viral Genome and Integrase Enzyme during HIV-1 Infection. J Virol, 95(2). (Read full article)
  7. Ward AE, Kiessling V, Pornillos O, White JM, Ganser-Pornillos BK, Tamm LK (2020). HIV-cell membrane fusion intermediates are restricted by Serincs as revealed by cryo-electron and TIRF microscopy. J Biol Chem, 295(45), 15183-15195. (Read full article)
  8. Christensen DE, Ganser-Pornillos BK, Johnson JS, Pornillos O, Sundquist WI (2020). Reconstitution and visualization of HIV-1 capsid-dependent replication and integration in vitro. Science, 370(6513). (Read full article)
  9. Yu A, Skorupka KA, Pak AJ, Ganser-Pornillos BK, Pornillos O, Voth GA (2020). TRIM5α self-assembly and compartmentalization of the HIV-1 viral capsid. Nat Commun, 11(1), 1307. (Read full article)
  10. Kucharska I, Ding P, Zadrozny KK, Dick RA, Summers MF, Ganser-Pornillos BK, Pornillos O (2020). Biochemical Reconstitution of HIV-1 Assembly and Maturation. J Virol, 94(5). (Read full article)
  11. Skorupka KA, Roganowicz MD, Christensen DE, Wan Y, Pornillos O, Ganser-Pornillos BK (2019). Hierarchical assembly governs TRIM5α recognition of HIV-1 and retroviral capsids. Sci Adv, 5(11), eaaw3631. (Read full article)
  12. Kueck T, Bloyet LM, Cassella E, Zang T, Schmidt F, Brusic V, Tekes G, Pornillos O, Whelan SPJ, Bieniasz PD (2019). Vesicular Stomatitis Virus Transcription Is Inhibited by TRIM69 in the Interferon-Induced Antiviral State. J Virol, 93(24). (Read full article)
  13. Stevens M, Franke B, Skorupka KA, Cafiso DS, Pornillos O, Mayans O, Norman DG (2019). Exploration of the TRIM Fold of MuRF1 Using EPR Reveals a Canonical Antiparallel Structure and Extended COS-Box. J Mol Biol, 431(15), 2900-2909. (Read full article)
  14. Sundquist WI, Pornillos O (2018). Retrovirus Restriction by TRIM5α: RINGside at a Cage Fight. Cell Host Microbe, 24(6), 751-753. (Read full article)
  15. Sanchez JG, Sparrer KMJ, Chiang C, Reis RA, Chiang JJ, Zurenski MA, Wan Y, Gack MU, Pornillos O (2018). TRIM25 Binds RNA to Modulate Cellular Anti-viral Defense. J Mol Biol, 430(24), 5280-5293. (Read full article)
  16. Buffone C, Martinez-Lopez A, Fricke T, Opp S, Severgnini M, Cifola I, Petiti L, Frabetti S, Skorupka K, Zadrozny KK, Ganser-Pornillos BK, Pornillos O, Di Nunzio F, Diaz-Griffero F (2018). Nup153 Unlocks the Nuclear Pore Complex for HIV-1 Nuclear Translocation in Nondividing Cells. J Virol, 92(19). (Read full article)
  17. Dick RA, Zadrozny KK, Xu C, Schur FKM, Lyddon TD, Ricana CL, Wagner JM, Perilla JR, Ganser-Pornillos BK, Johnson MC, Pornillos O, Vogt VM (2018). Inositol phosphates are assembly co-factors for HIV-1. Nature, 560(7719), 509-512. (Read full article)
  18. Wagner JM, Christensen DE, Bhattacharya A, Dawidziak DM, Roganowicz MD, Wan Y, Pumroy RA, Demeler B, Ivanov DN, Ganser-Pornillos BK, Sundquist WI, Pornillos O (2018). General Model for Retroviral Capsid Pattern Recognition by TRIM5 Proteins. J Virol, 92(4). (Read full article)
  19. Sparrer KMJ, Gableske S, Zurenski MA, Parker ZM, Full F, Baumgart GJ, Kato J, Pacheco-Rodriguez G, Liang C, Pornillos O, Moss J, Vaughan M, Gack MU (2017). TRIM23 mediates virus-induced autophagy via activation of TBK1. Nat Microbiol, 2(11), 1543-1557. (Read full article)
  20. Roganowicz MD, Komurlu S, Mukherjee S, Plewka J, Alam SL, Skorupka KA, Wan Y, Dawidowski D, Cafiso DS, Ganser-Pornillos BK, Campbell EM, Pornillos O (2017). TRIM5α SPRY/coiled-coil interactions optimize avid retroviral capsid recognition. PLoS Pathog, 13(10), e1006686. (Read full article)
  21. Dawidziak DM, Sanchez JG, Wagner JM, Ganser-Pornillos BK, Pornillos O (2017). Structure and catalytic activation of the TRIM23 RING E3 ubiquitin ligase. Proteins, 85(10), 1957-1961. (Read full article)
  22. Janczyk P, Skorupka KA, Tooley JG, Matson DR, Kestner CA, West T, Pornillos O, Stukenberg PT (2016). Mechanism of Ska Recruitment by Ndc80 Complexes to Kinetochores. Dev Cell, 41(4), 438-449.e4. (Read full article)
  23. Sanchez JG, Chiang JJ, Sparrer KMJ, Alam SL, Chi M, Roganowicz MD, Sankaran B, Gack MU, Pornillos O (2016). Mechanism of TRIM25 Catalytic Activation in the Antiviral RIG-I Pathway. Cell Rep, 16(5), 1315-1325. (Read full article)
  24. Wagner JM, Zadrozny KK, Chrustowicz J, Purdy MD, Yeager M, Ganser-Pornillos BK, Pornillos O (2016). Crystal structure of an HIV assembly and maturation switch. Elife, 5. (Read full article)
  25. Wagner JM, Roganowicz MD, Skorupka K, Alam SL, Christensen D, Doss G, Wan Y, Frank GA, Ganser-Pornillos BK, Sundquist WI, Pornillos O (2016). Mechanism of B-box 2 domain-mediated higher-order assembly of the retroviral restriction factor TRIM5α. Elife, 5. (Read full article)
  26. Li YL, Chandrasekaran V, Carter SD, Woodward CL, Christensen DE, Dryden KA, Pornillos O, Yeager M, Ganser-Pornillos BK, Jensen GJ, Sundquist WI (2016). Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids. Elife, 5. (Read full article)
  27. Gres AT, Kirby KA, KewalRamani VN, Tanner JJ, Pornillos O, Sarafianos SG (2015). STRUCTURAL VIROLOGY. X-ray crystal structures of native HIV-1 capsid protein reveal conformational variability. Science, 349(6243), 99-103. (Read full article)
  28. Bhattacharya A, Alam SL, Fricke T, Zadrozny K, Sedzicki J, Taylor AB, Demeler B, Pornillos O, Ganser-Pornillos BK, Diaz-Griffero F, Ivanov DN, Yeager M (2014). Structural basis of HIV-1 capsid recognition by PF74 and CPSF6. Proc Natl Acad Sci U S A, 111(52), 18625-30. (Read full article)
  29. Sanchez JG, Okreglicka K, Chandrasekaran V, Welker JM, Sundquist WI, Pornillos O (2014). The tripartite motif coiled-coil is an elongated antiparallel hairpin dimer. Proc Natl Acad Sci U S A, 111(7), 2494-9. (Read full article)
  30. Pornillos O, Ganser-Pornillos BK, Yeager M (2010). Atomic-level modelling of the HIV capsid. Nature, 469(7330), 424-7. (Read full article)
  31. Ganser-Pornillos BK, Chandrasekaran V, Pornillos O, Sodroski JG, Sundquist WI, Yeager M (2010). Hexagonal assembly of a restricting TRIM5alpha protein. Proc Natl Acad Sci U S A, 108(2), 534-9. (Read full article)
  32. Pornillos O, Ganser-Pornillos BK, Banumathi S, Hua Y, Yeager M (2010). Disulfide bond stabilization of the hexameric capsomer of human immunodeficiency virus. J Mol Biol, 401(5), 985-95. (Read full article)
  33. Zhu D, Hensel J, Hilgraf R, Abbasian M, Pornillos O, Deyanat-Yazdi G, Hua XH, Cox S (2009). Inhibition of protein kinase CK2 expression and activity blocks tumor cell growth. Mol Cell Biochem, 333(1-2), 159-67. (Read full article)
  34. Pornillos O, Ganser-Pornillos BK, Kelly BN, Hua Y, Whitby FG, Stout CD, Sundquist WI, Hill CP, Yeager M (2009). X-ray structures of the hexameric building block of the HIV capsid. Cell, 137(7), 1282-92. (Read full article)
  35. Chen YJ, Pornillos O, Lieu S, Ma C, Chen AP, Chang G (2007). X-ray structure of EmrE supports dual topology model. Proc Natl Acad Sci U S A, 104(48), 18999-9004. (Read full article)
  36. Zhang Z, Ma C, Pornillos O, Xiu X, Chang G, Saier MH Jr (2007). Functional characterization of the heterooligomeric EbrAB multidrug efflux transporter of Bacillus subtilis. Biochemistry, 46(17), 5218-25. (Read full article)
  37. Pornillos O, Chen YJ, Chen AP, Chang G (2005). X-ray structure of the EmrE multidrug transporter in complex with a substrate. Science, 310(5756), 1950-3. (Read full article)
  38. Pornillos O, Higginson DS, Stray KM, Fisher RD, Garrus JE, Payne M, He GP, Wang HE, Morham SG, Sundquist WI (2003). HIV Gag mimics the Tsg101-recruiting activity of the human Hrs protein. J Cell Biol, 162(3), 425-34. (Read full article)
  39. Pornillos O, Alam SL, Davis DR, Sundquist WI (2002). Structure of the Tsg101 UEV domain in complex with the PTAP motif of the HIV-1 p6 protein. Nat Struct Biol, 9(11), 812-7. (Read full article)
  40. Pornillos O, Alam SL, Rich RL, Myszka DG, Davis DR, Sundquist WI (2002). Structure and functional interactions of the Tsg101 UEV domain. EMBO J, 21(10), 2397-406. (Read full article)
  41. Jenkins Y, Pornillos O, Rich RL, Myszka DG, Sundquist WI, Malim MH (2001). Biochemical analyses of the interactions between human immunodeficiency virus type 1 Vpr and p6(Gag). J Virol, 75(21), 10537-42. (Read full article)
  42. Garrus JE, von Schwedler UK, Pornillos O, Morham SG, Zavitz KH, Wang HE, Wettstein DA, Stray KM, Ct M, Rich RL, Myszka DG, Sundquist WI (2001). Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding. Cell, 107(1), 55-65. (Read full article)

Review

  1. Ganser-Pornillos BK, Pornillos O (2019). Restriction of HIV-1 and other retroviruses by TRIM5. [Review]. Nat Rev Microbiol, 17(9), 546-556. (Read full article)
  2. Pornillos O, Ganser-Pornillos BK (2019). Maturation of retroviruses. [Review]. Curr Opin Virol, 36, 47-55. (Read full article)
  3. Ganser-Pornillos BK, Yeager M, Pornillos O (2012). Assembly and architecture of HIV. [Review]. Adv Exp Med Biol, 726, 441-65. (Read full article)
  4. Pornillos O, Chang G (2005). Inverted repeat domains in membrane proteins. [Review]. FEBS Lett, 580(2), 358-62. (Read full article)
  5. Pornillos O, Garrus JE, Sundquist WI (2002). Mechanisms of enveloped RNA virus budding. [Review]. Trends Cell Biol, 12(12), 569-79. (Read full article)