Jesse Rowley

Jesse Rowley, PhD

Languages spoken: English

Academic Information

Departments Primary - Internal Medicine

Divisions:

Academic Office Information

jesse.rowley@u2m2.utah.edu

Lab

My research objective is to understand how platelets and megakaryocytes influence human health and disease. Our work integrates cutting-edge multi-omics approaches with traditional molecular, cellular, and in vivo approaches to define mechanisms of platelet function, and uncover new avenues for platelet diagnostics and therapeutics.

Research Statement

<p>My research objective is to understand how platelets and megakaryocytes influence human health and disease. Our work integrates cutting-edge multi-omics approaches with traditional molecular, cellular, and in vivo approaches to define mechanisms of platelet function, and uncover new avenues for platelet diagnostics and therapeutics.</p>

Education History

Postdoctoral Fellowship The Johns Hopkins University
 Postdoctoral Fellow
University of Utah
 Postdoctoral Fellow
Research Fellow University of Utah
 Research Fellow
The Johns Hopkins University
 PhD
Research Fellow Johns Hopkins University
 Research Fellow
Brigham Young University
 BS

Selected Publications

Journal Article

  1. Grichine A, Jacob SP, Eckly AE, Villaret J, Joubert C, Appaix F, Pezet M, Ribba AS, Denarier E, Mazzega J, Rinckel JY, Lafanechre L, Elena-Herrmann B, Rowley JW, Sadoul K (2023). The fate of mitochondria during platelet activation. Blood Adv.
  2. Manne BK, Campbell RA, Bhatlekar S, Ajanel A, Denorme F, Portier I, Middleton EA, Tolley ND, Kosaka Y, Montenont E, Guo L, Rowley JW, Bray PF, Jacob SP, Fukanaga R, Proud CG, Weyrich AS, Rondina MT (2022). MAPK-interacting kinase 1 (Mnk1) regulates platelet production, activation, and thrombosis. Blood, 140(23), 2477-2489.
  3. Zhou C, Uluisik R, Rowley JW, David C, Jones CL, Scharer CD, Noetzli L, Fisher MH, Kirkpatrick GD, Bark K, Boss JM, Henry CJ, Pietras EM, Di Paola J, Porter CC (2022). Germline ETV6 mutation promotes inflammation and disrupts lymphoid development of early hematopoietic progenitors. Exp Hematol, 112-113, 24-34.
  4. Stoller ML, Basak I, Denorme F, Rowley JW, Alsobrooks J, Parsawar K, Nieman MT, Yost CC, Hamilton JR, Bray PF, Campbell RA (2021). Neutrophil cathepsin G proteolysis of protease-activated receptor 4 generates a novel, functional tethered ligand. Blood Adv, 6(7), 2303-2308.
  5. Wurtzel JGT, Lazar S, Sikder S, Cai KQ, Astsaturov I, Weyrich AS, Rowley JW, Goldfinger LE (2021). Platelet microRNAs inhibit primary tumor growth via broad modulation of tumor cell mRNA expression in ectopic pancreatic cancer in mice. PLoS One, 16(12), e0261633.
  6. Bhatlekar S, Jacob S, Manne BK, Guo L, Denorme F, Tugolukova EA, Cody MJ, Kosaka Y, Rigoutsos I, Campbell RA, Rowley JW, OConnell RM, Bray PF (2021). Megakaryocyte-specific knockout of the Mir-99b/let7e/125a cluster lowers platelet count without altering platelet function. Blood Cells Mol Dis, 92, 102624.
  7. Schwertz H, Rowley JW, Portier I, Middleton EA, Tolley ND, Campbell RA, Eustes AS, Chen K, Rondina MT (2021). Human platelets display dysregulated sepsis-associated autophagy, induced by altered LC3 protein-protein interaction of the Vici-protein EPG5. Autophagy, 18(7), 1-17.
  8. Shen Z, Du W, Perkins C, Fechter L, Natu V, Maecker H, Rowley J, Gotlib J, Zehnder J, Krishnan A (2021). Platelet transcriptome identifies progressive markers and potential therapeutic targets in chronic myeloproliferative neoplasms. Cell Rep Med, 2(10), 100425.
  9. Montenont E, Bhatlekar S, Jacob S, Kosaka Y, Manne BK, Lee O, Parra-Izquierdo I, Tugolukova E, Tolley ND, Rondina MT, Bray PF, Rowley JW (2020). CRISPR-edited megakaryocytes for rapid screening of platelet gene functions. Blood Adv, 5(9), 2362-2374.
  10. Eustes AS, Campbell RA, Middleton EA, Tolley ND, Manne BK, Montenont E, Rowley JW, Krauel K, Blair A, Guo L, Kosaka Y, Medeiros-de-Moraes IM, Lacerda M, Hottz ED, Neto HCF, Zimmerman GA, Weyrich AS, Petrey A, Rondina MT (2021). Heparanase expression and activity are increased in platelets during clinical sepsis. J Thromb Haemost, 19(5), 1319-1330.
  11. Guo L, Shen S, Rowley JW, Tolley ND, Jia W, Manne BK, McComas KN, Bolingbroke B, Kosaka Y, Krauel K, Denorme F, Jacob SP, Eustes AS, Campbell RA, Middleton EA, He X, Brown SM, Morrell CN, Weyrich AS, Rondina MT (2021). Platelet MHC Class I Mediates CD8+ T Cell Suppression During Sepsis. Blood, 138(5), 401-416.
  12. Melki I, Allaeys I, Tessandier N, Mailhot B, Cloutier N, Campbell RA, Rowley JW, Salem D, Zufferey A, Laroche A, Lvesque T, Patey N, Rauch J, Lood C, Droit A, McKenzie SE, Machlus KR, Rondina MT, Lacroix S, Fortin PR, Boilard E (2020). FcγRIIA expression accelerates nephritis and increases platelet activation in systemic lupus erythematosus. Blood, 136(25), 2933-2945.
  13. Bhatlekar S, Manne BK, Basak I, Edelstein LC, Tugolukova E, Stoller ML, Cody MJ, Morley SC, Nagalla S, Weyrich AS, Rowley JW, OConnell RM, Rondina MT, Campbell RA, Bray PF (2020). miR-125a-5p regulates megakaryocyte proplatelet formation via the actin-bundling protein L-plastin. Blood, 136(15), 1760-1772.
  14. Fisher MH, Kirkpatrick GD, Stevens B, Jones C, Callaghan M, Rajpurkar M, Fulbright J, Cooper MA, Rowley J, Porter CC, Gutierrez-Hartmann A, Jones K, Jordan C, Pietras EM, Di Paola J (2020). ETV6 germline mutations cause HDAC3/NCOR2 mislocalization and upregulation of interferon response genes. JCI Insight, 5(18).
  15. Manne BK, Denorme F, Middleton EA, Portier I, Rowley JW, Stubben C, Petrey AC, Tolley ND, Guo L, Cody M, Weyrich AS, Yost CC, Rondina MT, Campbell RA (2020). Platelet gene expression and function in patients with COVID-19. Blood, 136(11), 1317-1329.
  16. Melki I, Allaeys I, Tessandier N, Mailhot B, Cloutier N, Campbell RA, Rowley JW, Salem D, Zufferey A, Laroche A, Lvesque T, Patey N, Rauch J, Lood C, Droit A, McKenzie SE, Machlus KR, Rondina MT, Lacroix S, Fortin P, Boilard E (2020). FcγRIIA expression aggravates nephritis and increases platelet activation in systemic lupus erythematosus in mice. Blood.
  17. Rondina MT, Voora D, Simon LM, Schwertz H, Harper JF, Lee O, Bhatlekar SC, Li Q, Eustes AS, Montenont E, Campbell RA, Tolley ND, Kosaka Y, Weyrich AS, Bray PF, Rowley JW (2019). Longitudinal RNA-Seq Analysis of the Repeatability of Gene Expression and Splicing in Human Platelets Identifies a Platelet SELP Splice QTL. Circ Res, 126(4), 501-516.
  18. Bhatlekar S, Basak I, Edelstein LC, Campbell RA, Lindsey CR, Italiano JE Jr, Weyrich AS, Rowley JW, Rondina MT, Sola-Visner M, Bray PF (2019). Anti-apoptotic BCL2L2 increases megakaryocyte proplatelet formation in cultures of human cord blood. Haematologica, 104(10), 2075-2083.
  19. Middleton EA, Rowley JW, Campbell RA, Grissom CK, Brown SM, Beesley SJ, Schwertz H, Kosaka Y, Manne BK, Krauel K, Tolley ND, Eustes AS, Guo L, Paine R 3rd, Harris ES, Zimmerman GA, Weyrich AS, Rondina MT (2019). Sepsis alters the transcriptional and translational landscape of human and murine platelets. Blood, 134(12), 911-923.
  20. Campbell RA, Schwertz H, Hottz ED, Rowley JW, Manne BK, Washington AV, Hunter-Mellado R, Tolley ND, Christensen M, Eustes AS, Montenont E, Bhatlekar S, Ventrone CH, Kirkpatrick BD, Pierce KK, Whitehead SS, Diehl SA, Bray PF, Zimmerman GA, Kosaka Y, Bozza PT, Bozza FA, Weyrich AS, Rondina MT (2019). Human megakaryocytes possess intrinsic antiviral immunity through regulated induction of IFITM3. Blood, 133(19), 2013-2026.
  21. Denis HL, Lamontagne-Proulx J, St-Amour I, Mason SL, Rowley JW, Cloutier N, Tremblay M, Vincent AT, Gould PV, Chouinard S, Weyrich AS, Rondina MT, Barker RA, Boilard E, Cicchetti F (2018). Platelet abnormalities in Huntington's disease. J Neurol Neurosurg Psychiatry, 90(3), 272-283.
  22. Morales-Ortz J, Deal V, Reyes F, Maldonado-Martnez G, Ledesma N, Staback F, Croft C, Pacheco A, Ortiz-Zuazaga H, Yost CC, Rowley JW, Madera B, John AS, Chen J, Lopez J, Rondina MT, Hunter R, Gibson A, Washington AV (2018). Platelet-derived TLT-1 is a prognostic indicator in ALI/ARDS and prevents tissue damage in the lungs in a mouse model. Blood, 132(23), 2495-2505.
  23. Schwertz H, Rowley JW, Schumann GG, Thorack U, Campbell RA, Manne BK, Zimmerman GA, Weyrich AS, Rondina MT (2018). Endogenous LINE-1 (Long Interspersed Nuclear Element-1) Reverse Transcriptase Activity in Platelets Controls Translational Events Through RNA-DNA Hybrids. Arterioscler Thromb Vasc Biol, 38(4), 801-815.
  24. Campbell RA, Franks Z, Bhatnagar A, Rowley JW, Manne BK, Supiano MA, Schwertz H, Weyrich AS, Rondina MT (2018). Granzyme A in Human Platelets Regulates the Synthesis of Proinflammatory Cytokines by Monocytes in Aging. J Immunol, 200(1), 295-304.
  25. Schwertz H, Rowley JW, Zimmerman GA, Weyrich AS, Rondina MT (2017). Retinoic acid receptor-α regulates synthetic events in human platelets. J Thromb Haemost, 15(12), 2408-2418.
  26. Fidler TP, Rowley JW, Araujo C, Boudreau LH, Marti A, Souvenir R, Dale K, Boilard E, Weyrich AS, Abel ED (2017). Superoxide Dismutase 2 is dispensable for platelet function. Thromb Haemost, 117(10), 1859-1867.
  27. Campbell RA, Vieira-de-Abreu A, Rowley JW, Franks ZG, Manne BK, Rondina MT, Kraiss LW, Majersik JJ, Zimmerman GA, Weyrich AS (2017). Clots Are Potent Triggers of Inflammatory Cell Gene Expression: Indications for Timely Fibrinolysis. Arterioscler Thromb Vasc Biol, 37(10), 1819-1827.
  28. Fidler TP, Middleton EA, Rowley JW, Boudreau LH, Campbell RA, Souvenir R, Funari T, Tessandier N, Boilard E, Weyrich AS, Abel ED (2017). Glucose Transporter 3 Potentiates Degranulation and Is Required for Platelet Activation. Arterioscler Thromb Vasc Biol, 37(9), 1628-1639.
  29. Michael JV, Wurtzel JGT, Mao GF, Rao AK, Kolpakov MA, Sabri A, Hoffman NE, Rajan S, Tomar D, Madesh M, Nieman MT, Yu J, Edelstein LC, Rowley JW, Weyrich AS, Goldfinger LE (2017). Platelet microparticles infiltrating solid tumors transfer miRNAs that suppress tumor growth. Blood, 130(5), 567-580.
  30. Nance D, Campbell RA, Rowley JW, Downie JM, Jorde LB, Kahr WH, Mereby SA, Tolley ND, Zimmerman GA, Weyrich AS, Rondina MT (2016). Combined variants in factor VIII and prostaglandin synthase-1 amplify hemorrhage severity across three generations of descendants. J Thromb Haemost, 14(11), 2230-2240.
  31. Yost CC, Schwertz H, Cody MJ, Wallace JA, Campbell RA, Vieira-de-Abreu A, Araujo CV, Schubert S, Harris ES, Rowley JW, Rondina MT, Fulcher JM, Koening CL, Weyrich AS, Zimmerman GA (2016). Neonatal NET-inhibitory factor and related peptides inhibit neutrophil extracellular trap formation. J Clin Invest, 126(10), 3783-3798.
  32. Rondina MT, Freitag M, Pluthero FG, Kahr WH, Rowley JW, Kraiss LW, Franks Z, Zimmerman GA, Weyrich AS, Schwertz H (2016). Non-genomic activities of retinoic acid receptor alpha control actin cytoskeletal events in human platelets. J Thromb Haemost, 14(5), 1082-94.
  33. Rowley JW, Chappaz S, Corduan A, Chong MM, Campbell R, Khoury A, Manne BK, Wurtzel JG, Michael JV, Goldfinger LE, Mumaw MM, Nieman MT, Kile BT, Provost P, Weyrich AS (2016). Dicer1-mediated miRNA processing shapes the mRNA profile and function of murine platelets. Blood, 127(14), 1743-51.
  34. Noetzli L, Lo RW, Lee-Sherick AB, Callaghan M, Noris P, Savoia A, Rajpurkar M, Jones K, Gowan K, Balduini C, Pecci A, Gnan C, De Rocco D, Doubek M, Li L, Lu L, Leung R, Landolt-Marticorena C, Hunger S, Heller P, Gutierrez-Hartmann A, Xiayuan L, Pluthero FG, Rowley JW, Weyrich AS, Kahr WHA, Porter CC, Di Paola J (2015). Germline mutations in ETV6 are associated with thrombocytopenia, red cell macrocytosis and predisposition to lymphoblastic leukemia. Nat Genet, 47(5), 535-538.
  35. Shi DS, Smith MC, Campbell RA, Zimmerman PW, Franks ZB, Kraemer BF, Machlus KR, Ling J, Kamba P, Schwertz H, Rowley JW, Miles RR, Liu ZJ, Sola-Visner M, Italiano JE Jr, Christensen H, Kahr WH, Li DY, Weyrich AS (2014). Proteasome function is required for platelet production. J Clin Invest, 124(9), 3757-66.
  36. Sturrock A, Mir-Kasimov M, Baker J, Rowley J, Paine R 3rd (2014). Key role of microRNA in the regulation of granulocyte macrophage colony-stimulating factor expression in murine alveolar epithelial cells during oxidative stress. J Biol Chem, 289(7), 4095-105.
  37. McInturff AM, Cody MJ, Elliott EA, Glenn JW, Rowley JW, Rondina MT, Yost CC (2012). Mammalian target of rapamycin regulates neutrophil extracellular trap formation via induction of hypoxia-inducible factor 1 α. Blood, 120(15), 3118-25.
  38. Thon JN, Peters CG, Machlus KR, Aslam R, Rowley J, Macleod H, Devine MT, Fuchs TA, Weyrich AS, Semple JW, Flaumenhaft R, Italiano JE Jr (2012). T granules in human platelets function in TLR9 organization and signaling. J Cell Biol, 198(4), 561-74.
  39. Rowley JW, Finn AV, French PA, Jennings LK, Bluestein D, Gross PL, Freedman JE, Steinhubl SR, Zimmerman GA, Becker RC, Dauerman HL, Smyth SS, 2011 Platelet Colloquium Participants (2012). Cardiovascular devices and platelet interactions: understanding the role of injury, flow, and cellular responses. Circ Cardiovasc Interv, 5(2), 296-304.
  40. Rowley JW, Oler AJ, Tolley ND, Hunter BN, Low EN, Nix DA, Yost CC, Zimmerman GA, Weyrich AS (2011). Genome-wide RNA-seq analysis of human and mouse platelet transcriptomes. Blood, 118(14), e101-11.
  41. Kahr WH, Hinckley J, Li L, Schwertz H, Christensen H, Rowley JW, Pluthero FG, Urban D, Fabbro S, Nixon B, Gadzinski R, Storck M, Wang K, Ryu GY, Jobe SM, Schutte BC, Moseley J, Loughran NB, Parkinson J, Weyrich AS, Di Paola J (2011). Mutations in NBEAL2, encoding a BEACH protein, cause gray platelet syndrome. Nat Genet, 43(8), 738-40.
  42. Pawlinski R, Wang JG, Owens AP 3rd, Williams J, Antoniak S, Tencati M, Luther T, Rowley JW, Low EN, Weyrich AS, Mackman N (2010). Hematopoietic and nonhematopoietic cell tissue factor activates the coagulation cascade in endotoxemic mice. Blood, 116(5), 806-14.
  43. Rowley J, Monie A, Hung CF, Wu TC (2009). Expression of IL-15RA or an IL-15/IL-15RA fusion on CD8+ T cells modifies adoptively transferred T-cell function in cis. Eur J Immunol, 39(2), 491-506.
  44. Rowley J, Monie A, Hung CF, Wu TC (2008). Inhibition of tumor growth by NK1.1+ cells and CD8+ T cells activated by IL-15 through receptor beta/common gamma signaling in trans. J Immunol, 181(12), 8237-47.
  45. Lin KY, Lu D, Hung CF, Peng S, Huang L, Jie C, Murillo F, Rowley J, Tsai YC, He L, Kim DJ, Jaffee E, Pardoll D, Wu TC (2007). Ectopic expression of vascular cell adhesion molecule-1 as a new mechanism for tumor immune evasion. Cancer Res, 67(4), 1832-41.

Review

  1. Davizon-Castillo P, Rowley JW, Rondina MT (2020). Megakaryocyte and Platelet Transcriptomics for Discoveries in Human Health and Disease. [Review]. Arterioscler Thromb Vasc Biol, ATVBAHA119313280.
  2. Schubert S, Weyrich AS, Rowley JW (2014). A tour through the transcriptional landscape of platelets. [Review]. Blood, 124(4), 493-502.
  3. Rowley JW, Schwertz H, Weyrich AS (2012). Platelet mRNA: the meaning behind the message. [Review]. Curr Opin Hematol, 19(5), 385-91.

Book Chapter

  1. Rowley JW, Weyrich AS, Bray PF (2019). The Platelet Transcriptome in Health and Disease. In Platelets (4th edition, pp. 139-153). San Diego, CA. USA: Elsevier Science Publishing Co Inc.
  2. Jesse W Rowley, Bhanukanth K Manne, Andrew S Weyrich (2017). The Platelet Transcriptome: Coding RNAs. In Gresele, P., Kleiman, N.S., Lopez, J.A., Page, C.P. (Eds.), Platelets in Thrombotic and Non-Thrombotic Disorders (1). Springer International Publishing.
  3. Schwertz H, Rowley JW, Tolley ND, Campbell RA, Weyrich AS (2012). Assessing protein synthesis by platelets. In Methods Mol Biol (788, pp. 141-53). United States.

Editorial

  1. Middleton EA, Rowley JW (2021). eQTLs in platelets and iPSC-megakaryocytes. Blood, 137(7), 869-870.

Letter

  1. Rowley JW, Weyrich AS (2013). Coordinate expression of transcripts and proteins in platelets. [Letter to the editor]. Blood, 121(26), 5255-6.