Wesley I. Sundquist

Wesley I. Sundquist, PhD

Languages spoken: English

Academic Information

Departments Primary - Biochemistry

Academic Office Information

wes@biochem.utah.edu

Lab

Wesley Sundquist, PhD, is Samuels Professor and Co-Chair of the Department of Biochemistry at the University of Utah. He is also a member of the Cell Response and Regulation Program at Huntsman Cancer Institute.

Sundquist received a bachelor's degree from Carleton College, Minnesota, and a PhD from the Massachusetts Institute of Technology. His honors and awards include election to the American Academy of Arts and Sciences and the National Academy of Sciences

Research Statement

The Sundquist lab studies the cellular, molecular and structural biology of retroviruses, particularly HIV, and the roles of the ESCRT pathway in cell division and the abscission checkpoint. Major projects in our lab include studies of: 1) ESCRT pathway functions and regulation in cell division and cancer, 2) HIV budding, 3) HIV capsid structure, function and restriction, particularly by the TRIM5α system, 4) AAV egress, and 5) Designed enveloped protein nanoparticles. Our approaches include NMR, EM, crystallographic and computational studies of viral complexes, identification and biochemical analyses of the interactions between viral components and their cellular partners, and genetic analyses of viral and cellular protein functions.

Education History

Undergraduate Carleton College, Northfield, MN
 BA
Doctoral Training Massachusetts Institute of Technology, MA
 PhD
Postdoctoral Fellowship MRC Laboratory of Molecular Biology
 Postdoctoral Fellow

Selected Publications

Journal Article

  1. Lamorte L, Titolo S, Lemke CT, Goudreau N, Mercier JF, Wardrop E, Shah VB, von Schwedler UK, Langelier C, Banik SS, Aiken C, Sundquist WI, Mason SW (2013). Discovery of novel small-molecule HIV-1 replication inhibitors that stabilize capsid complexes. Antimicrob Agents Chemother, 57(10), 4622-31. (Read full article)
  2. Kelly BN, Howard BR, Wang H, Robinson H, Sundquist WI, Hill CP (2006). Implications for viral capsid assembly from crystal structures of HIV-1 Gag(1-278) and CA(N)(133-278). Biochemistry, 45(38), 11257-66. (Read full article)
  3. Sundquist WI, Lippard SJ, Stollar B (1986). Binding of cis- and trans-diamminedichloroplatinum(II) to deoxyribonucleic acid exposes nucleosides as measured immunochemically with anti-nucleoside antibodies. Biochemistry, 25(7), 1520-4. (Read full article)
  4. Morita E, Arii J, Christensen D, Votteler J, Sundquist W (2012). Attenuated protein expression vectors for use in siRNA rescue experiments. BioTechniques, 0(0), 1-5. (Read full article)
  5. De Domenico I, Ward DM, Langelier C, Vaughn MB, Nemeth E, Sundquist WI, Ganz T, Musci G, Kaplan (2007). The molecular mechanism of hepcidin-mediated ferroportin down-regulation. Molecular biology of the cell, 18(7), 2569-78. (Read full article)
  6. Bajorek M, Morita E, Skalicky JJ, Morham SG, Babst M, Sundquist W (2009). Biochemical analyses of human IST1 and its function in cytokinesis. Molecular biology of the cell, 20(5), 1360-73. (Read full article)
  7. Massiah MA, Worthylake D, Christensen AM, Sundquist WI, Hill CP, Summers M (1996). Comparison of the NMR and X-ray structures of the HIV-1 matrix protein: evidence for conformational changes during viral assembly. Protein science, 5(12), 2391-8. (Read full article)
  8. Vajdos FF, Yoo S, Houseweart M, Sundquist WI, Hill C (1997). Crystal structure of cyclophilin A complexed with a binding site peptide from the HIV-1 capsid protein. Protein science, 6(11), 2297-307. (Read full article)
  9. Hollis LS, Sundquist WI, Burstyn JN, Heiger-Bernays WJ, Bellon SF, Ahmed KJ, Amundsen AR, Stern EW, Lippard S (1991). Mechanistic studies of a novel class of trisubstituted platinum(II) antitumor agents. Cancer research, 51(7), 1866-75. (Read full article)
  10. Gamble TR, Vajdos FF, Yoo S, Worthylake DK, Houseweart M, Sundquist WI, Hill C (1996). Crystal structure of human cyclophilin A bound to the amino-terminal domain of HIV-1 capsid. Cell, 87(7), 1285-94. (Read full article)
  11. Garrus JE, von Schwedler UK, Pornillos OW, Morham SG, Zavitz KH, Wang HE, Wettstein DA, Stray KM, Côté M, Rich RL, Myszka DG, Sundquist W (2001). Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding. Cell, 107(1), 55-65. (Read full article)
  12. von Schwedler UK, Stuchell M, Müller B, Ward DM, Chung HY, Morita E, Wang HE, Davis T, He GP, Cimbora DM, Scott A, Kräusslich HG, Kaplan J, Morham SG, Sundquist W (2003). The protein network of HIV budding. Cell, 114(6), 701-13. (Read full article)
  13. Fisher RD, Chung HY, Zhai Q, Robinson H, Sundquist WI, Hill C (2007). Structural and biochemical studies of ALIX/AIP1 and its role in retrovirus budding. Cell, 128(5), 841-52. (Read full article)
  14. Pornillos O, Ganser-Pornillos BK, Kelly BN, Hua Y, Whitby FG, Stout CD, Sundquist WI, Hill CP, Yeager (2009). X-ray structures of the hexameric building block of the HIV capsid. Cell, 137(7), 1282-92. (Read full article)
  15. Rheinemann L, Downhour DM, Bredbenner K, Mercenne G, Davenport KA, Schmitt PT, Necessary CR, McCullough J, Schmitt AP, Simon SM, Sundquist WI, Elde N (2021). RetroCHMP3 blocks budding of enveloped viruses without blocking cytokinesis. Cell, 184(21), 5419-5431.e16. (Read full article)
  16. von Schwedler UK, Stemmler TL, Klishko VY, Li S, Albertine KH, Davis DR, Sundquist W (1998). Proteolytic refolding of the HIV-1 capsid protein amino-terminus facilitates viral core assembly. The EMBO journal, 17(6), 1555-68. (Read full article)
  17. Pornillos O, Alam SL, Rich RL, Myszka DG, Davis DR, Sundquist W (2002). Structure and functional interactions of the Tsg101 UEV domain. The EMBO journal, 21(10), 2397-406. (Read full article)
  18. Ganser BK, Cheng A, Sundquist WI, Yeager (2003). Three-dimensional structure of the M-MuLV CA protein on a lipid monolayer: a general model for retroviral capsid assembly. The EMBO journal, 22(12), 2886-92. (Read full article)
  19. Alam SL, Sun J, Payne M, Welch BD, Blake BK, Davis DR, Meyer HH, Emr SD, Sundquist W (2004). Ubiquitin interactions of NZF zinc fingers. The EMBO journal, 23(7), 1411-21. (Read full article)
  20. Scott A, Chung HY, Gonciarz-Swiatek M, Hill GC, Whitby FG, Gaspar J, Holton JM, Viswanathan R, Ghaffarian S, Hill CP, Sundquist W (2005). Structural and mechanistic studies of VPS4 proteins. The EMBO journal, 24(20), 3658-69. (Read full article)
  21. Wright ER, Schooler JB, Ding HJ, Kieffer C, Fillmore C, Sundquist WI, Jensen G (2007). Electron cryotomography of immature HIV-1 virions reveals the structure of the CA and SP1 Gag shells. The EMBO journal, 26(8), 2218-26. (Read full article)
  22. Morita E, Sandrin V, Chung HY, Morham SG, Gygi SP, Rodesch CK, Sundquist W (2007). Human ESCRT and ALIX proteins interact with proteins of the midbody and function in cytokinesis. The EMBO journal, 26(19), 4215-27. (Read full article)
  23. Arrigoni R, Alam SL, Wamstad JA, Bardwell VJ, Sundquist WI, Schreiber-Agus (2006). The Polycomb-associated protein Rybp is a ubiquitin binding protein. FEBS letters, 580(26), 6233-41. (Read full article)
  24. Kempe T, Kent SB, Chow F, Peterson SM, Sundquist WI, L'Italien JJ, Harbrecht D, Plunkett D, DeLorbe W (1985). Multiple-copy genes: production and modification of monomeric peptides from large multimeric fusion proteins. Gene, 39(2-3), 239-45. (Read full article)
  25. Ren T, Bancroft DP, Sundquist WI, Keck M, Masschelein A, Lippar S (1993). Synthesis, Characterization, DNA Binding Properties, and Solution Thermochromism of Platinum (II) Complexes of the Ethidium Cation: Altered Regioselectivity in a DNA-Promoted Reaction. Journal of the American Chemical Society, 115(24), 11341-52.
  26. Sundquist WI, Bancroft DP, Chassot L, Lippard S (1988). DNA promotes the reaction of cis-diamminedichloroplatinum(II) with the exocyclic amino groups of ethidium bromide. Journal of the American Chemical Society, 110(25), 8559¿8560.
  27. Sundquist WI, Bancroft DP, Lippard S (1990). Synthesis, Characterization, and Biological Activity of cis-Diammineplatinum(II) Complexes of the DNA Intercalators 9-Aminoacridine and Chloroquine. Journal of the American Chemical Society, 112(4), 1590-6.
  28. Bowler BE, Ahmed KJ, Sundquist WI, Hollis LS, Whang EE, Lippard S (1989). Synthesis, Characterization, and DNA Binding Properties of (1,2-Diaminoethane)-platinum(II) Complexes Linked to the DNA Intercalator Acridine Orange by Trimethylene and Hexamethylene Chains. Journal of the American Chemical Society, 111(4), 1299-306.
  29. Wang B, Alam SL, Meyer HH, Payne M, Stemmler TL, Davis DR, Sundquist W (2003). Structure and ubiquitin interactions of the conserved zinc finger domain of Npl4. The Journal of biological chemistry, 278(22), 20225-34. (Read full article)
  30. Fisher RD, Wang B, Alam SL, Higginson DS, Robinson H, Sundquist WI, Hill C (2003). Structure and ubiquitin binding of the ubiquitin-interacting motif. The Journal of biological chemistry, 278(31), 28976-84. (Read full article)
  31. Stuchell MD, Garrus JE, Müller B, Stray KM, Ghaffarian S, McKinnon R, Kräusslich HG, Morham SG, Sundquist W (2004). The human endosomal sorting complex required for transport (ESCRT-I) and its role in HIV-1 budding. The Journal of biological chemistry, 279(34), 36059-71. (Read full article)
  32. Ward DM, Vaughn MB, Shiflett SL, White PL, Pollock AL, Hill J, Schnegelberger R, Sundquist WI, Kaplan (2005). The role of LIP5 and CHMP5 in multivesicular body formation and HIV-1 budding in mammalian cells. The Journal of biological chemistry, 280(11), 10548-55. (Read full article)
  33. Higa MM, Alam SL, Sundquist WI, Ullman K (2007). Molecular characterization of the Ran-binding zinc finger domain of Nup153. The Journal of biological chemistry, 282(23), 17090-100. (Read full article)
  34. Skalicky JJ, Arii J, Wenzel DM, Stubblefield WM, Katsuyama A, Uter NT, Bajorek M, Myszka DG, Sundquist W (2012). Interactions of the human LIP5 regulatory protein with endosomal sorting complexes required for transport. The Journal of biological chemistry, 287(52), 43910-26. (Read full article)
  35. Han H, Monroe N, Votteler J, Shakya B, Sundquist WI, Hill C (2015). Binding of Substrates to the Central Pore of the Vps4 ATPase Is Autoinhibited by the Microtubule Interacting and Trafficking (MIT) Domain and Activated by MIT Interacting Motifs (MIMs). The Journal of biological chemistry, 290(21), 13490-9. (Read full article)
  36. Han H, Schubert HL, McCullough J, Monroe N, Purdy MD, Yeager M, Sundquist WI, Hill C (2020). Structure of spastin bound to a glutamate-rich peptide implies a hand-over-hand mechanism of substrate translocation. The Journal of biological chemistry, 295(2), 435-443. (Read full article)
  37. Rheinemann L, Thompson T, Mercenne G, Paine EL, Peterson FC, Volkman BF, Alam SL, Alian A, Sundquist W (2021). Interactions between AMOT PPxY motifs and NEDD4L WW domains function in HIV-1 release. The Journal of biological chemistry, 297(2), 100975. (Read full article)
  38. Pornillos O, Higginson DS, Stray KM, Fisher RD, Garrus JE, Payne M, He GP, Wang HE, Morham SG, Sundquist W (2003). HIV Gag mimics the Tsg101-recruiting activity of the human HRS protein. The Journal of cell biology, 162(3), 425-34. (Read full article)
  39. Christensen AM, Massiah MA, Turner BG, Sundquist WI, Summers M (1996). Three-dimensional structure of the HTLV-II matrix protein and comparative analysis of matrix proteins from the different classes of pathogenic human retroviruses. Journal of molecular biology, 264(5), 1117-31. (Read full article)
  40. Yoo S, Myszka DG, Yeh C, McMurray M, Hill CP, Sundquist W (1997). Molecular recognition in the HIV-1 capsid/cyclophilin A complex. Journal of molecular biology, 269(5), 780-95. (Read full article)
  41. Benjamin J, Ganser-Pornillos BK, Tivol WF, Sundquist WI, Jensen G (2005). Three-dimensional structure of HIV-1 virus-like particles by electron cryotomography. Journal of molecular biology, 346(2), 577-88. (Read full article)
  42. Kelly BN, Kyere S, Kinde I, Tang C, Howard BR, Robinson H, Sundquist WI, Summers MF, Hill C (2007). Structure of the antiviral assembly inhibitor CAP-1 complex with the HIV-1 CA protein. Journal of molecular biology, 373(2), 355-66. (Read full article)
  43. Yu Z, Gonciarz MD, Sundquist WI, Hill CP, Jensen G (2008). Cryo-EM structure of dodecameric Vps4p and its 2:1 complex with Vta1p. Journal of molecular biology, 377(2), 364-77. (Read full article)
  44. Gonciarz MD, Whitby FG, Eckert DM, Kieffer C, Heroux A, Sundquist WI, Hill C (2008). Biochemical and structural studies of yeast Vps4 oligomerization. Journal of molecular biology, 384(4), 878-95. (Read full article)
  45. Monroe N, Han H, Gonciarz MD, Eckert DM, Karren MA, Whitby FG, Sundquist WI, Hill C (2014). The oligomeric state of the active Vps4 AAA ATPase. Journal of molecular biology, 426(3), 510-25. (Read full article)
  46. Massiah MA, Starich MR, Paschall C, Summers MF, Christensen AM, Sundquist W (1994). Three-dimensional structure of the human immunodeficiency virus type 1 matrix protein. Journal of molecular biology, 244(2), 198-223. (Read full article)
  47. Hollis LS, Miller AV, Amundsen AR, Stern EW, Sundquist WI, Toney J, Burstyn JN, Heiger W, Lippard S (1989). Chemical and biological studies of new platinum antitumor agents. Journal of inorganic biochemistry, 36(3-4), 151-372.
  48. Jenkins Y, Pornillos O, Rich RL, Myszka DG, Sundquist WI, Malim M (2001). Biochemical analyses of the interactions between human immunodeficiency virus type 1 Vpr and p6(Gag). Journal of virology, 75(21), 10537-42. (Read full article)
  49. Forshey BM, von Schwedler U, Sundquist WI, Aiken (2002). Formation of a human immunodeficiency virus type 1 core of optimal stability is crucial for viral replication. Journal of virology, 76(11), 5667-77. (Read full article)
  50. von Schwedler UK, Stray KM, Garrus JE, Sundquist W (2003). Functional surfaces of the human immunodeficiency virus type 1 capsid protein. Journal of virology, 77(9), 5439-50. (Read full article)
  51. Ganser-Pornillos BK, von Schwedler UK, Stray KM, Aiken C, Sundquist W (2004). Assembly properties of the human immunodeficiency virus type 1 CA protein. Journal of virology, 78(5), 2545-52. (Read full article)
  52. Hatziioannou T, Cowan S, Von Schwedler UK, Sundquist WI, Bieniasz P (2004). Species-specific tropism determinants in the human immunodeficiency virus type 1 capsid. Journal of virology, 78(11), 6005-12. (Read full article)
  53. Schmitt AP, Leser GP, Morita E, Sundquist WI, Lamb R (2005). Evidence for a new viral late-domain core sequence, FPIV, necessary for budding of a paramyxovirus. Journal of virology, 79(5), 2988-97. (Read full article)
  54. Langelier C, von Schwedler UK, Fisher RD, De Domenico I, White PL, Hill CP, Kaplan J, Ward D, Sundquist W (2006). Human ESCRT-II complex and its role in human immunodeficiency virus type 1 release. Journal of virology, 80(19), 9465-80. (Read full article)
  55. Chung HY, Morita E, von Schwedler U, Müller B, Kräusslich HG, Sundquist W (2008). NEDD4L overexpression rescues the release and infectivity of human immunodeficiency virus type 1 constructs lacking PTAP and YPXL late domains. Journal of virology, 82(10), 4884-97. (Read full article)
  56. Langelier CR, Sandrin V, Eckert DM, Christensen DE, Chandrasekaran V, Alam SL, Aiken C, Olsen JC, Kar AK, Sodroski JG, Sundquist W (2008). Biochemical characterization of a recombinant TRIM5alpha protein that restricts human immunodeficiency virus type 1 replication. Journal of virology, 82(23), 11682-94. (Read full article)
  57. Zhai Q, Landesman MB, Robinson H, Sundquist WI, Hill C (2011). Identification and structural characterization of the ALIX-binding late domains of simian immunodeficiency virus SIVmac239 and SIVagmTan-1. Journal of virology, 85(1), 632-7. (Read full article)
  58. Zhai Q, Landesman MB, Chung HY, Dierkers A, Jeffries CM, Trewhella J, Hill CP, Sundquist W (2011). Activation of the retroviral budding factor ALIX. Journal of virology, 85(17), 9222-6. (Read full article)
  59. Lemke CT, Titolo S, von Schwedler U, Goudreau N, Mercier JF, Wardrop E, Faucher AM, Coulombe R, Banik SS, Fader L, Gagnon A, Kawai SH, Rancourt J, Tremblay M, Yoakim C, Simoneau B, Archambault J, Sundquist WI, Mason S (2012). Distinct effects of two HIV-1 capsid assembly inhibitor families that bind the same site within the N-terminal domain of the viral CA protein. Journal of virology, 86(12), 6643-55. (Read full article)
  60. Wagner JM, Christensen DE, Bhattacharya A, Dawidziak DM, Roganowicz MD, Wan Y, Pumroy RA, Demeler B, Ivanov DN, Ganser-Pornillos BK, Sundquist WI, Pornillos (2018). General model for retroviral capsid pattern recognition by TRIM5 Proteins. Journal of virology, 92(4), (Read full article)
  61. Li S, Hill CP, Sundquist WI, Finch J (2000). Image reconstructions of helical assemblies of the HIV-1 CA protein. Nature, 407(6802), 409-13. (Read full article)
  62. Stuchell-Brereton MD, Skalicky JJ, Kieffer C, Karren MA, Ghaffarian S, Sundquist W (2007). ESCRT-III recognition by VPS4 ATPases. Nature, 449(7163), 740-4. (Read full article)
  63. Jäger S, Cimermancic P, Gulbahce N, Johnson JR, McGovern KE, Clarke SC, Shales M, Mercenne G, Pache L, Li K, Hernandez H, Jang GM, Roth SL, Akiva E, Marlett J, Stephens M, D'Orso I, Fernandes J, Fahey M, Mahon C, O'Donoghue AJ, Todorovic A, Morris JH, Maltby DA, Alber T, Cagney G, Bushman FD, Young JA, Chanda SK, Sundquist WI, Kortemme T, Hernandez RD, Craik CS, Burlingame A, Sali A, Frankel AD, Krogan N (2011). Global landscape of HIV-human protein complexes. Nature, 481(7381), 365-70. (Read full article)
  64. Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King NP, Sundquist W (2016). Designed proteins induce the formation of nanocage-containing extracellular vesicles. Nature, 540(7632), 292-295. (Read full article)
  65. Sundquist WI, Klug (1989). Telomeric DNA dimerizes by formation of guanine tetrads between hairpin loops. Nature, 342(6251), 825-9. (Read full article)
  66. Link JO, Rhee MS, Tse WC, Zheng J, Somoza JR, Rowe W, Begley R, Chiu A, Mulato A, Hansen D, Singer E, Tsai LK, Bam RA, Chou CH, Canales E, Brizgys G, Zhang JR, Li J, Graupe M, Morganelli P, Liu Q, Wu Q, Halcomb RL, Saito RD, Schroeder SD, Lazerwith SE, Bondy S, Jin D, Hung M, Novikov N, Liu X, Villaseñor AG, Cannizzaro CE, Hu EY, Anderson RL, Appleby TC, Lu B, Mwangi J, Liclican A, Niedziela-Majka A, Papalia GA, Wong MH, Leavitt SA, Xu Y, Koditek D, Stepan GJ, Yu H, Pagratis N, Clancy S, Ahmadyar S, Cai TZ, Sellers S, Wolckenhauer SA, Ling J, Callebaut C, Margot N, Ram RR, Liu YP, Hyland R, Sinclair GI, Ruane PJ, Crofoot GE, McDonald CK, Brainard DM, Lad L, Swaminathan S, Sundquist WI, Sakowicz R, Chester AE, Lee WE, Daar ES, Yant SR, Cihlar (2020). Clinical targeting of HIV capsid protein with a long-acting small molecule. Nature, 584(7822), 614-618. (Read full article)
  67. Kempe T, Sundquist WI, Chow F, Hu S (1985). Chemical and enzymatic biotin-labeling of oligodeoxyribonucleotides. Nucleic acids research, 13(1), 45-57. (Read full article)
  68. Kempe T, Chow F, Sundquist WI, Nardi TJ, Paulson B, Peterson S (1982). Selective 2'-benzoylation at the cis 2',3'-diols of protected ribonucleosides. New solid phase synthesis of RNA and DNA-RNA mixtures. Nucleic acids research, 10(21), 6695-714. (Read full article)
  69. Sundquist WI, Heaphy (1993). Evidence for interstrand quadruplex formation in the dimerization of human immunodeficiency virus 1 genomic RNA. Proceedings of the National Academy of Sciences of the United States of America, 90(8), 3393-7. (Read full article)
  70. Hill CP, Worthylake D, Bancroft DP, Christensen AM, Sundquist W (1996). Crystal structures of the trimeric human immunodeficiency virus type 1 matrix protein: implications for membrane association and assembly. Proceedings of the National Academy of Sciences of the United States of America, 93(7), 3099-104. (Read full article)
  71. Bosco DA, Eisenmesser EZ, Pochapsky S, Sundquist WI, Kern (2002). Catalysis of cis/trans isomerization in native HIV-1 capsid by human cyclophilin A. Proceedings of the National Academy of Sciences of the United States of America, 99(8), 5247-52. (Read full article)
  72. Scott A, Gaspar J, Stuchell-Brereton MD, Alam SL, Skalicky JJ, Sundquist W (2005). Structure and ESCRT-III protein interactions of the MIT domain of human VPS4A. Proceedings of the National Academy of Sciences of the United States of America, 102(39), 13813-8. (Read full article)
  73. Stremlau M, Perron M, Lee M, Li Y, Song B, Javanbakht H, Diaz-Griffero F, Anderson DJ, Sundquist WI, Sodroski (2006). Specific recognition and accelerated uncoating of retroviral capsids by the TRIM5alpha restriction factor. Proceedings of the National Academy of Sciences of the United States of America, 103(14), 5514-9. (Read full article)
  74. McCullough J, Fisher RD, Whitby FG, Sundquist WI, Hill C (2008). ALIX-CHMP4 interactions in the human ESCRT pathway. Proceedings of the National Academy of Sciences of the United States of America, 105(22), 7687-91. (Read full article)
  75. Morita E, Colf LA, Karren MA, Sandrin V, Rodesch CK, Sundquist W (2010). Human ESCRT-III and VPS4 proteins are required for centrosome and spindle maintenance. Proceedings of the National Academy of Sciences of the United States of America, 107(29), 12889-94. (Read full article)
  76. Schubert HL, Zhai Q, Sandrin V, Eckert DM, Garcia-Maya M, Saul L, Sundquist WI, Steiner RA, Hill C (2010). Structural and functional studies on the extracellular domain of BST2/tetherin in reduced and oxidized conformations. Proceedings of the National Academy of Sciences of the United States of America, 107(42), 17951-6. (Read full article)
  77. Ganser-Pornillos BK, Chandrasekaran V, Pornillos O, Sodroski JG, Sundquist WI, Yeager (2011). Hexagonal assembly of a restricting TRIM5alpha protein. Proceedings of the National Academy of Sciences of the United States of America, 108(2), 534-9. (Read full article)
  78. Sanchez JG, Okreglicka K, Chandrasekaran V, Welker JM, Sundquist WI, Pornillos (2014). The tripartite motif coiled-coil is an elongated antiparallel hairpin dimer. Proceedings of the National Academy of Sciences of the United States of America, 111(7), 2494-9. (Read full article)
  79. Gu M, LaJoie D, Chen OS, von Appen A, Ladinsky MS, Redd MJ, Nikolova L, Bjorkman PJ, Sundquist WI, Ullman KS, Frost (2017). LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in fission yeast and human cells. Proceedings of the National Academy of Sciences of the United States of America, 114(11), E2166-E2175. (Read full article)
  80. Sadler JB, Wenzel D, Williams LK, Guindo-Martínez M, Alam SL, Mercader JM, Torrents D, Ullman KS, Sundquist WI, Martin-Serrano J (2018). A cancer-associated polymorphism in ESCRT-III disrupts the abscission checkpoint and promotes genome instabilityDOI: 10.1073/pnas.1805504115. Proceedings of the National Academy of Sciences of the United States of America, 115(38), E8900-E8908.
  81. Sundquist WI, Lippard SJ, Stollar B (1987). Monoclonal antibodies to DNA modified with cis- or trans-diamminedichloroplatinum(II). Proceedings of the National Academy of Sciences of the United States of America, 84(23), 8225-9. (Read full article)
  82. Gitti RK, Lee BM, Walker J, Summers MF, Yoo S, Sundquist W (1996). Structure of the amino-terminal core domain of the HIV-1 capsid protein. Science (New York, N.Y.), 273(5272), 231-5. (Read full article)
  83. Gamble TR, Yoo S, Vajdos FF, von Schwedler UK, Worthylake DK, Wang H, McCutcheon JP, Sundquist WI, Hill C (1997). Structure of the carboxyl-terminal dimerization domain of the HIV-1 capsid protein. Science (New York, N.Y.), 278(5339), 849-53. (Read full article)
  84. Ganser BK, Li S, Klishko VY, Finch JT, Sundquist W (1999). Assembly and analysis of conical models for the HIV-1 core. Science (New York, N.Y.), 283(5398), 80-3. (Read full article)
  85. McCullough J, Clippinger AK, Talledge N, Skowyra ML, Saunders MG, Naismith TV, Colf LA, Afonine P, Arthur C, Sundquist WI, Hanson PI, Frost (2015). Structure and membrane remodeling activity of ESCRT-III helical polymers. Science (New York, N.Y.), 350(6267), 1548-51. (Read full article)
  86. Christensen DE, Ganser-Pornillos BK, Johnson JS, Pornillos O, Sundquist W (2020). Reconstitution and visualization of HIV-1 capsid-dependent replication and integration in vitro. Science (New York, N.Y.), 370(6513), eabc8420. (Read full article)
  87. Pornillos O, Alam SL, Davis DR, Sundquist W (2002). Structure of the Tsg101 UEV domain in complex with the PTAP motif of the HIV-1 p6 protein. Nature structural biology, 9(11), 812-7. (Read full article)
  88. Howard BR, Vajdos FF, Li S, Sundquist WI, Hill C (2003). Structural insights into the catalytic mechanism of cyclophilin A. Nature structural biology, 10(6), 475-81. (Read full article)
  89. Yant SR, Mulato A, Hansen D, Tse WC, Niedziela-Majka A, Zhang JR, Stepan GJ, Jin D, Wong MH, Perreira JM, Singer E, Papalia GA, Hu EY, Zheng J, Lu B, Schroeder SD, Chou K, Ahmadyar S, Liclican A, Yu H, Novikov N, Paoli E, Gonik D, Ram RR, Hung M, McDougall WM, Brass AL, Sundquist WI, Cihlar T, Link J (2019). A highly potent long-acting small-molecule HIV-1 capsid inhibitor with efficacy in a humanized mouse model. Nature medicine, 25(9), 1377-1384. (Read full article)
  90. Sundquist WI, Schubert HL, Kelly BN, Hill GC, Holton JM, Hill C (2004). Ubiquitin recognition by the human TSG101 protein. Molecular cell, 13(6), 783-9. (Read full article)
  91. Worthylake DK, Wang H, Yoo S, Sundquist WI, Hill C (1999). Structures of the HIV-1 capsid protein dimerization domain at 2.6 A resolution. Acta crystallographica. Section D, Biological crystallography, 55(Pt 1), 85-92. (Read full article)
  92. Effantin G, Dordor A, Sandrin V, Martinelli N, Sundquist WI, Schoehn G, Weissenhorn (2013). ESCRT-III CHMP2A and CHMP3 form variable helical polymers in vitro and act synergistically during HIV-1 budding. Cellular microbiology, 15(2), 213-26. (Read full article)
  93. Sundquist WI, Ahmed, KJ, Hollis LS, Lippard S (1987). Solvolysis reactions of cis- and trans-diamminedichloroplatinum(II) in dimethyl sulfoxide. Structural characterization and DNA binding of trans-bis(ammine)chloro(DMSO)platinum(1+). Inorganic chemistry, 26(10), 1524-8.
  94. Kieffer C, Skalicky JJ, Morita E, De Domenico I, Ward DM, Kaplan J, Sundquist W (2008). Two distinct modes of ESCRT-III recognition are required for VPS4 functions in lysosomal protein targeting and HIV-1 budding. Developmental cell, 15(1), 62-73. (Read full article)
  95. Dowlatshahi DP, Sandrin V, Vivona S, Shaler TA, Kaiser SE, Melandri F, Sundquist WI, Kopito R (2012). ALIX is a Lys63-specific polyubiquitin binding protein that functions in retrovirus budding. Developmental cell, 23(6), 1247-54. (Read full article)
  96. Alam SL, Langelier C, Whitby FG, Koirala S, Robinson H, Hill CP, Sundquist W (2006). Structural basis for ubiquitin recognition by the human ESCRT-II EAP45 GLUE domain. Nature structural & molecular biology, 13(11), 1029-30. (Read full article)
  97. Zhai Q, Fisher RD, Chung HY, Myszka DG, Sundquist WI, Hill C (2008). Structural and functional studies of ALIX interactions with YPX(n)L late domains of HIV-1 and EIAV. Nature structural & molecular biology, 15(1), 43-9. (Read full article)
  98. Bajorek M, Schubert HL, McCullough J, Langelier C, Eckert DM, Stubblefield WM, Uter NT, Myszka DG, Hill CP, Sundquist W (2009). Structural basis for ESCRT-III protein autoinhibition. Nature structural & molecular biology, 16(7), 754-62. (Read full article)
  99. Nguyen HC, Talledge N, McCullough J, Sharma A, Moss FR 3rd, Iwasa JH, Vershinin MD, Sundquist WI, Frost (2020). Membrane constriction and thinning by sequential ESCRT-III polymerization. Nature structural & molecular biology, 27(4), 392-399. (Read full article)
  100. Sandrin V, Sundquist W (2013). ESCRT requirements for EIAV budding. Retrovirology, 10, 104. (Read full article)
  101. Zhai Q, Landesman MB, Robinson H, Sundquist WI, Hill C (2011). Structure of the Bro1 domain protein BROX and functional analyses of the ALIX Bro1 domain in HIV-1 budding. PloS one, 6(12), e27466. (Read full article)
  102. Morita E, Sandrin V, Alam SL, Eckert DM, Gygi SP, Sundquist W (2007). Identification of human MVB12 proteins as ESCRT-I subunits that function in HIV budding. Cell host & microbe, 2(1), 41-53. (Read full article)
  103. Neil SJ, Sandrin V, Sundquist WI, Bieniasz P (2007). An interferon-alpha-induced tethering mechanism inhibits HIV-1 and Ebola virus particle release but is counteracted by the HIV-1 Vpu protein. Cell host & microbe, 2(3), 193-203. (Read full article)
  104. Morita E, Sandrin V, McCullough J, Katsuyama A, Baci Hamilton I, Sundquist W (2011). ESCRT-III protein requirements for HIV-1 budding. Cell host & microbe, 9(3), 235-242. (Read full article)
  105. Mercenne G, Alam SL, Arii J, Lalonde MS, Sundquist W (2015). Angiomotin functions in HIV-1 assembly and budding. eLife, 4, e03778. (Read full article)
  106. Caballe A, Wenzel DM, Agromayor M, Alam SL, Skalicky JJ, Kloc M, Carlton JG, Labrador L, Sundquist WI, Martin-Serrano (2015). ULK3 regulates cytokinetic abscission by phosphorylating ESCRT-III proteins. eLife, 4, e06547. (Read full article)
  107. Wagner JM, Roganowicz MD, Skorupka K, Alam SL, Christensen D, Doss G, Wan Y, Frank GA, Ganser-Pornillos BK, Sundquist WI, Pornillos (2016). Mechanism of B-box 2 domain-mediated higher-order assembly of the retroviral restriction factor TRIM5α. eLife, 5, e16309. (Read full article)
  108. Li YL, Chandrasekaran V, Carter SD, Woodward CL, Christensen DE, Dryden KA, Pornillos O, Yeager M, Ganser-Pornillos BK, Jensen GJ, Sundquist W (2016). Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids. eLife, 5, e16269. (Read full article)
  109. Monroe N, Han H, Shen PS, Sundquist WI, Hill C (2017). Structural basis of protein translocation by the Vps4-Vta1 AAA ATPase. eLife, 6, e24487. (Read full article)
  110. Han H, Monroe N, Sundquist WI, Shen PS, Hill C (2017). The AAA ATPase Vps4 binds ESCRT-III substrates through a repeating array of dipeptide-binding pockets. eLife, 6, 510-25. (Read full article)
  111. Han H, Fulcher JM, Dandey VP, Iwasa JH, Sundquist WI, Kay MS, Shen PS, Hill C (2019). Structure of Vps4 with circular peptides and implications for translocation of two polypeptide chains by AAA+ ATPases. eLife, 8, e44071. (Read full article)
  112. Strohacker LD, Mackay DR, Whitney MA, Couldwells GC, Sundquist WI, Ullman KS (2021). Identification of abscission checkpoint bodies as structures that regulate ESCRT factors to control abscission timing. eLife, 10, (Read full article)
  113. Huttunen M, Samolej J, Evans RJ, Yakimovich A, White IJ, Kriston-Vizi J, Martin-Serrano J, Sundquist WI, Frickel EM, Mercer (2021). Vaccinia virus hijacks ESCRT-mediated multivesicular body formation for virus egress. Life science alliance, 4(8), e202000910. (Read full article)
  114. Wenzel DM, Mackay DR, Skalicky JJ, Paine EL, Miller MS, Ullman KS, Sundquist W (2022). Comprehensive analysis of the human ESCRT-III-MIT domain interactome reveals new cofactors for cytokinetic abscission. eLife, 11(e), 77779.
  115. Rheinemann L, Downhour DM, Davenport KA, McKeown AN, Sundquist WI, Elde N (2022). Recurrent evolution of an inhibitor of ESCRT-dependent virus budding and LINE-1 retrotransposition in primates. Current biology, 32(7), 1511-1522.e6. (Read full article)
  116. Paine EL, Skalicky JJ, Whitby FG, Mackay DR, Ullman KS, Hill CP, Sundquist W (2023). The Calpain-7 protease functions together with the ESCRT-III protein IST1 within the midbody to regulate the timing and completion of abscission. eLife, 12e, 84515.

Review

  1. McCullough J, Colf LA, Sundquist WI (2013). Membrane fission reactions of the mammalian ESCRT pathway. [Review]. Annu Rev Biochem, 82, 663-92. (Read full article)
  2. Li S, Ganser B, Hill CP, Finch JT, Sundquist W (2001). Structural Models for the HIV-1 Capsid. Virus research, 77(2), 97-99.
  3. Ganser-Pornillos BK, Yeager M, Sundquist W (2008). The structural biology of HIV assembly. Current opinion in structural biology, 18(2), 203-17. (Read full article)
  4. Morita E, Sundquist W (2004). Retrovirus budding. Annual review of cell and developmental biology, 20, 395-425. (Read full article)
  5. McCullough J, Frost A, Sundquist W (2018). Structures, Functions, and Dynamics of ESCRT-III/Vps4 Membrane Remodeling and Fission Complexes. Annual review of cell and developmental biology, 34, 85-109. (Read full article)
  6. Pornillos O, Garrus JE, Sundquist W (2002). Mechanisms of enveloped RNA virus budding. Trends in cell biology, 12(12), 569-79. (Read full article)
  7. Votteler J, Sundquist W (2013). Virus budding and the ESCRT pathway. Cell host & microbe, 14(3), 232-41. (Read full article)
  8. Sundquist WI, Lippard S (1990). The Coordination Chemistry of Platinum Anticancer Drugs and Related Compounds with DNA. Coordination chemistry reviews, 100th Vol Celebration Issue, 293-322.
  9. Swanstrom R, Sundquist W (2021). Stephan Oroszlan and the Proteolytic Processing of Retroviral Proteins: Following A Pro. Viruses, 13(11), (Read full article)
  10. Sundquist WI, Kräusslich H (2012). HIV-1 assembly, budding, and maturation. Cold Spring Harbor perspectives in medicine, 2(7), a006924. (Read full article)
  11. Sundquist W (1991). The Structures of Telomeric DNA. Nucleic acids and molecular biology, 5, 1-24.

Commentary

  1. Gu Y, Sundquist W (2003). Good to CU. Nature, 424(6944), 21-2. (Read full article)
  2. Alam SL, Sundquist W (2007). Structural biology: ESCRT service. Nature, 447(7147), 921-2. (Read full article)
  3. Sundquist W (1993). Conducting the G Quartet. Current biology, 3(12), 893-5.
  4. Nogales E, Sundquist W (2007). Macromolecular Assemblages: focusing in on complexity. Current opinion in structural biology, 17(2), 205-8.
  5. Alam SL, Sundquist W (2006). Two new structures of Ub-receptor complexes. U2. Nature structural & molecular biology, 13(3), 186-8. (Read full article)
  6. McCullough J, Sundquist W (2014). Putting a finger in the ring. Nature structural & molecular biology, 21(12), 1025-7. (Read full article)
  7. Sundquist WI, Corb (2016). Creating a robust research enterprise. http://www.asbmb.org/asbmbtoday/201609/PublicAffairs/. 15(8), 35.
  8. Sundquist WI, Matthews R (2016). Staff scientists in the workforce. http://www.asbmb.org/asbmbtoday/201610/PublicAffairs. 15(9), 32-3.
  9. Sundquist W (2011). Commentary on “The protein network of HIV budding”. Yegor Voronin (Ed.), HIV: 30 years in 30 weeks. Web,

Editorial

  1. Rey FA, Sundquist W (2013). Macromolecular assemblies. Current opinion in structural biology, 23(2), 224-8. (Read full article)
  2. Rey FA, Sundquist W (2011). Macromolecular assemblages. Current opinion in structural biology, 21(2), 221-2. (Read full article)
  3. Sundquist WI, Krug R (2012). Assemble, replicate, remodel and evade. Current opinion in virology, 2(2), 111-4. (Read full article)

Other

  1. Lalonde MS, Sundquist W (2012). How HIV finds the door. Proceedings of the National Academy of Sciences of the United States of America, 109(46), 18631-2. (Read full article)
  2. Pickett CL, Corb BW, Matthews CR, Sundquist WI, Berg J (2015). Toward a sustainable biomedical research enterprise: Finding consensus and implementing recommendations. Proceedings of the National Academy of Sciences of the United States of America, 112(35), 10832-6. (Read full article)
  3. Sundquist WI, Ullman K (2016). An ESCRT to seal the envelope. Science (New York, N.Y.), 6241(348), 1314-1315.
  4. McCullough J, Sundquist W (2020). Membrane Remodeling: ESCRT-III Filaments as Molecular Garrotes. Current biology, 30(23), R1425-R1428. (Read full article)
  5. Sundquist W (1996). Tattle tales. Nature structural biology, 3(1), 8-11. (Read full article)
  6. Schaller MD, McDowell G, Porter A, Shippen D, Friedman KL, Gentry MS, Serio TR, Sundquist W (2017). What's in a name?. eLife, 6, (Read full article)