Janis J. Weis

Janis J. Weis, PhD

Languages spoken: English

Academic Information

Departments Primary - Pathology

Lab

Research Statement

The research in my laboratory is focused on understanding the means by which microbial infections result in acute and chronic inflammatory pathologies. We use the murine model of Lyme disease to assess the development of arthritis following infection by the spirochete Borrelia burgdorferi. We are taking two complementary approaches to identifying host responses that both permit chronic infection and result in arthritis. The first approach has centered on identifying the tissue-specific response to B. burgdorferi and its products that results in arthritis development. Comparison of responses in mouse strains destined to develop severe disease has revealed an exaggerated induction of interferon-inducible genes. In C3H mice this response is due to Type I IFN, is transient, and is clearly correlated with the subsequent infiltration of myeloid cells character¬istic of this arthritis. The ensuing synovial hyperproliferation in the infected tissue is also characteristic of human disease. Type I IFN is strongly associated with other inflammatory pathologies including Lupus and has been found in a subset of patients with Rheumatoid Arthritis. Intriguingly, this response is absent from mouse strains displaying mild arthritis, and the differential response can be studied in relevant cells from the joint tissue. Current analysis involves genetic and epigenetic regulation of the IFN locus, and the possible involvement of microRNAs in this response. A second model for chronic Lyme arthritis involves mice lacking the non-redundant inflammatory cytokine IL-10. In the absence of IL-10, arthritis is driven by a classic T cell dependent Type II IFN response to localized bacteria. Analysis of tissue infiltrate into the joint and use of cytokine reporter mice have revealed inflammatory cells responsible for arthritis regulation and for the development of arthritis in dysregulated situations. This model has many similarities to patients with chronic Lyme Disease.

We also utilize the mouse for the identi¬fication of the genetic elements that regulate the severity of Lyme arthritis. Epidemiological studies in humans support the existence of polymor¬phic genes that determine the severity of disease, and indicate that alleles of these genes may also set the stage for chronic conditions potentially involving autoimmune pathways. Intercross populations between mildly and severely arthritic strains of mice revealed multiple genetic loci (Quantitative Trait Loci or QTL) on five chromosomes of the mouse that regulate the severity of disease. The development of Interval Specific Congenic Lines of mice has allowed isolation of these loci and demonstration of associated penetrant arthritis phenotypes. Further delineation of the regions of interest through the development of recombinant congenics has led to the characterization of multiple sub-loci and reduced the associated intervals to 1-2Mbp. We are currently are poised to isolate invidual loci on unique recombinant congenic mouse lines and assessment of contribution to arthritis severity. Analysis of available SNP databases has allowed selection of several candidate genes. These are currently being assessed for effect on arthritis severity by a combination of cell biology experi¬mentation and by the development of allele-specific transgenic mice.

Education History

Undergraduate University of Kansas
 BA
Doctoral Training University of Minnesota
 PhD
Postdoctoral Fellowship Harvard Medical School and Brigham and Women's Hospital
 Postdoctoral Fellow

Selected Publications

Journal Article

  1. Crandall H, Ma Y, Dunn DM, Sundsbak RS, Zachary JF, Olofsson P, Holmdahl R, Weis JH, Weiss RB, Teuscher C, Weis J (2005). Bb2Bb3 Regulation of Murine Lyme Arthritis Is Distinct from Ncf1 and Independent of the Phagocyte Nicotinamide Adenine Dinucleotide Phosphate Oxidase. The American journal of pathology, 167(3), 775-85. (Read full article)
  2. Bramwell KK, Ma Y, Weis JH, Teuscher C, Weis J (2012). High-throughput genotyping of advanced congenic lines by high resolution melting analysis for identification of Bbaa2, a QTL controlling Lyme arthritis. BioTechniques, 52(3), 183-90. (Read full article)
  3. Wang X, Ma Y, Yoder A, Crandall H, Zachary JF, Fujinami RS, Weis JH, Weis J (2008). T cell infiltration is associated with increased Lyme arthritis in TLR2-/- mice. FEMS immunology and medical microbiology, 52(1), 124-33. (Read full article)
  4. Rogers SW, Weis JJ, Ma Y, Teuscher C, Gahring L (2008). Mouse chromosome 11 harbors genetic determinants of hippocampal strain-specific nicotinic receptor expression. Hippocampus, 18(8), 750-7. (Read full article)
  5. Brown JP, Zachary JF, Teuscher C, Weis JJ, Wooten R (1999). Dual role of interleukin-10 in murine Lyme disease: regulation of arthritis severity and host defense. Infection and immunity, 67(10), 5142-50. (Read full article)
  6. Hirschfeld M, Weis JJ, Toshchakov V, Salkowski CA, Cody MJ, Ward DC, Qureshi N, Michalek SM, Vogel S (2001). Signaling by toll-like receptor 2 and 4 agonists results in differential gene expression in murine macrophages. Infection and immunity, 69(3), 1477-82. (Read full article)
  7. Wang X, Ma Y, Weis JH, Zachary JF, Kirschning CJ, Weis J (2005). Relative contributions of innate and acquired host responses to bacterial control and arthritis development in Lyme disease. Infection and immunity, 73(1), 657-60. (Read full article)
  8. Ma Y, Miller JC, Crandall H, Larsen ET, Dunn DM, Weiss RB, Subramanian M, Weis JH, Zachary JF, Teuscher C, Weis J (2009). Interval-specific congenic lines reveal quantitative trait Loci with penetrant lyme arthritis phenotypes on chromosomes 5, 11, and 12. Infection and immunity, 77(8), 3302-11. (Read full article)
  9. Miller JC, Maylor-Hagen H, Ma Y, Weis JH, Weis J (2010). The Lyme disease spirochete Borrelia burgdorferi utilizes multiple ligands, including RNA, for interferon regulatory factor 3-dependent induction of type I interferon-responsive genes. Infection and immunity, 78(7), 3144-53. (Read full article)
  10. Stewart, P.E., Wang, X., Bueschel DM, Clifton DR, Grimm, D., Tilly, K., Carroll JA, Weis JJ, Rosa P (2006). Delineating the requirement for the Borrelia burgdorferi virulence factor OspC in the mammalian host. Infection and immunity, (74), 3547-3553.
  11. Bramwell KK, Ma Y, Weis JH, Chen X, Zachary JF, Teuscher C, Weis J (2014). Lysosomal beta-glucuronidase regulates Lyme and rheumatoid arthritis severity. The Journal of clinical investigation, 124(1), 311-20. (Read full article)
  12. Morrison TB, Ma Y, Weis JH, Weis J (1999). Rapid and sensitive quantification of Borrelia burgdorferi-infected mouse tissues by continuous fluorescent monitoring of PCR. Journal of clinical microbiology, 37(4), 987-92. (Read full article)
  13. Weis JJ, McCracken BA, Ma Y, Fairbairn D, Roper RJ, Morrison TB, Weis JH, Zachary JF, Doerge RW, Teuscher (1999). Identification of quantitative trait loci governing arthritis severity and humoral responses in the murine model of Lyme disease. Journal of immunology (Baltimore, Md., 162(2), 948-56. (Read full article)
  14. Hirschfeld M, Kirschning CJ, Schwandner R, Wesche H, Weis JH, Wooten RM, Weis J (1999). Cutting edge: inflammatory signaling by Borrelia burgdorferi lipoproteins is mediated by toll-like receptor 2. Journal of immunology (Baltimore, Md., 163(5), 2382-6. (Read full article)
  15. Hirschfeld M, Ma Y, Weis JH, Vogel SN, Weis J (2000). Cutting edge: repurification of lipopolysaccharide eliminates signaling through both human and murine toll-like receptor 2. Journal of immunology (Baltimore, Md., 165(2), 618-22. (Read full article)
  16. Wooten RM, Ma Y, Yoder RA, Brown JP, Weis JH, Zachary JF, Kirschning CJ, Weis J (2002). Toll-like receptor 2 is required for innate, but not acquired, host defense to Borrelia burgdorferi. Journal of immunology (Baltimore, Md., 168(1), 348-55. (Read full article)
  17. Bolz DD, Sundsbak RS, Ma Y, Akira S, Kirschning CJ, Zachary JF, Weis JH, Weis J (2004). MyD88 plays a unique role in host defense but not arthritis development in Lyme disease. Journal of immunology (Baltimore, Md., 173(3), 2003-10. (Read full article)
  18. Miller JC, Ma Y, Bian J, Sheehan KC, Zachary JF, Weis JH, Schreiber RD, Weis J (2008). A critical role for type I IFN in arthritis development following Borrelia burgdorferi infection of mice. Journal of immunology (Baltimore, Md., 181(12), 8492-503. (Read full article)
  19. Sonderegger FL, Ma Y, Maylor-Hagan H, Brewster J, Huang X, Spangrude GJ, Zachary JF, Weis JH, Weis J (2012). Localized production of IL-10 suppresses early inflammatory cell infiltration and subsequent development of IFN-gamma-mediated Lyme arthritis. Journal of immunology (Baltimore, Md., 188(3), 1381-93. (Read full article)
  20. Lochhead RB, Sonderegger FL, Ma Y, Brewster JE, Cornwall D, Maylor-Hagen H, Miller JC, Zachary JF, Weis JH, Weis J (2012). Endothelial cells and fibroblasts amplify the arthritogenic type I IFN response in murine Lyme disease and are major sources of chemokines in Borrelia burgdorferi-infected joint tissue. Journal of immunology (Baltimore, Md., 189(5), 2488-501. (Read full article)
  21. Ma Y, Bramwell KK, Lochhead RB, Paquette JK, Zachary JF, Weis JH, Teuscher C, Weis J (2014). Borrelia burgdorferi arthritis-associated locus Bbaa1 regulates Lyme arthritis and K/BxN serum transfer arthritis through intrinsic control of type I IFN production. Journal of immunology (Baltimore, Md., 193(12), 6050-60. (Read full article)
  22. Bramwell KK, Mock K, Ma Y, Weis JH, Teuscher C, Weis J (2015). beta-Glucuronidase, a Regulator of Lyme Arthritis Severity, Modulates Lysosomal Trafficking and MMP-9 Secretion in Response to Inflammatory Stimuli. Journal of immunology (Baltimore, Md., 195(4), 1647-56. (Read full article)
  23. Paquette JK, Ma Y, Fisher C, Li J, Lee SB, Zachary JF, Kim YS, Teuscher C, Weis J (2017). Genetic Control of Lyme Arthritis by Borrelia burgdorferi Arthritis-Associated Locus 1 Is Dependent on Localized Differential Production of IFN-beta and Requires Upregulation of Myostatin. Journal of immunology (Baltimore, Md., 199(10), 3525-3534. (Read full article)
  24. Whiteside SK, Snook JP, Ma Y, Sonderegger FL, Fisher C, Petersen C, Zachary JF, Round JL, Williams MA, Weis J (2018). IL-10 Deficiency Reveals a Role for TLR2-Dependent Bystander Activation of T Cells in Lyme Arthritis. Journal of immunology (Baltimore, Md., 200(4), 1457-1470. (Read full article)
  25. Roper RJ, Weis JJ, McCracken BA, Green CB, Ma Y, Weber KS, Fairbairn D, Butterfield RJ, Potter MR, Zachary JF, Doerge RW, Teuscher (2001). Genetic control of susceptibility to experimental Lyme arthritis is polygenic and exhibits consistent linkage to multiple loci on chromosome 5 in four independent mouse crosses. Genes and immunity, 2(7), 388-97. (Read full article)
  26. Lochhead RB, Ma Y, Zachary JF, Baltimore D, Zhao JL, Weis JH, O'Connell RM, Weis J (2014). MicroRNA-146a Provides Feedback Regulation of Lyme Arthritis but Not Carditis during Infection with Borrelia burgdorferi. PLoS pathogens, 10(6), e1004212. (Read full article)
  27. Lochhead RB, Zachary JF, Dalla Rosa L, Ma Y, Weis JH, O'Connell RM, Weis J (2015). Antagonistic Interplay between MicroRNA-155 and IL-10 during Lyme Carditis and Arthritis. PloS one, 10(8), e0135142. (Read full article)

Review

  1. Bolz DD, Weis J (2004). Molecular mimicry to Borrelia burgdorferi: pathway to autoimmunity?. Autoimmunity, 37(5), 387-92. (Read full article)
  2. Miller JC, Ma Y, Crandall H, Wang X, Weis J (2008). Gene expression profiling provides insights into the pathways involved in inflammatory arthritis development: murine model of Lyme disease. Experimental and molecular pathology, 85(1), 20-7. (Read full article)
  3. Whiteside SK, Snook JP, Williams MA, Weis J (2018). Bystander T Cells: A Balancing Act of Friends and Foes. Trends in immunology, 39(12), 1021-1035. (Read full article)
  4. Bramwell KK, Teuscher C, Weis J (2014). Forward genetic approaches for elucidation of novel regulators of Lyme arthritis severity. Frontiers in cellular and infection microbiology, 4, 76. (Read full article)