Ordering Recommendation
• This is a broad epithelial autoantibody panel for initial diagnostic assessment in disorders that present with blistering, erosions, pruritus, and/or urticaria, which affect skin and mucous membranes, from suspected basement membrane zone antibody-associated disease including bullous pemphigoid, pemphigoid variants, epidermolysis bullosa acquisita, linear IgA bullous dermatosis, and linear IgA disease variants.
• Order concurrently with perilesional skin biopsy for direct immunofluorescence for initial diagnosis.
• Use for disease monitoring with semiquantitative antibody assessments and tracking.
• To screen for other immunobullous diseases in addition to basement membrane zone antibody-associated diseases, order concurrently with the Pemphigus Panel, IgA cell surface antibodies indirect immunofluorescence individual test, Dermatitis Herpetiformis IgA Panel, as clinically indicated.
Components
- IgA basement membrane zone antibodies by indirect immunofluorescence
- IgG basement membrane zone antibodies by indirect immunofluorescence
- IgG BP 180 and IgG BP 230 antibody levels by ELISAs
- IgG collagen VII antibody level by ELISA
Methodology
Indirect immunofluorescence and enzyme-linked immunosorbent assay (ELISA)
Performed
Daily
Reported
4-9 business days
New York Department of Health (NYDOH) Approval Status
This test is New York DOH approved except IgG Collagen VII ELISA; see Pemphigoid Panel as NY DOH-approved alternative.
Information Required to Submit with Order
Completed Immunodermatology Laboratory requisition form and copies of both sides of insurance cards or completed billing information.
CPT Code(s)
88346; 88350 x 3; 83516 x 3 (86256 x 1-4 possible)
SPECIMEN REQUIREMENTS
Collect Blood
Plain red top tube or serum separator tube.
Specimen Preparation
2 mL serum separated from blood into serum vial provided in kit (Minimum needed: 0.5 mL)
Storage/Transport Temperature
Room temperature
Conditions with Disclaimer for Potentially Anomalous Results
Hemolyzed or lipemic serum specimens, whole blood
Stability
Ambient: 7 days
Refrigerated: 14 days
Frozen: Indefinitely