Skip to main content

IMMUNODERMATOLOGY LABORATORY TEST MENU

The Immunodermatology Laboratory offers tests on skin and mucosal tissue specimens and serum specimens that aid in the diagnosis and management of many types of dermatological conditions and diseases. Tissue testing is more sensitive than serum testing in several diseases, and the findings from tissue testing may prompt serum testing to distinguish certain diseases and establish antibody levels for therapeutic tracking. Serum testing includes Antibody Panels and Specific Antibody Assays to identify disease-related immunological markers and patterns of autoantibody reactivity. Serum Antibody Panel Testing is most useful for initial diagnostic testing, assessing changing disease presentations, and periodically evaluating antibody expression profiles in established diseases. Specific antibody assays may be helpful in the diagnosis of certain diseases and indicated for monitoring disease activity. Of note, ELISA testing detects serum antibodies to specific epitopes in major target antigens, and patients may develop antibodies to other epitopes or other epithelial antigens; therefore, the combination of indirect immunofluorescence and ELISA testing can provide more comprehensive immunopathological information that either alone.

Please contact us if you have any questions about our testing.

Tissue Specimens

Tissue Specimens

Direct immunofluorescence (DIF): IgG, IgG4, IgM, IgA, C3, and fibrinogen; upon request other IgG subclasses (IgG1, IgG2, IgG3), another complement component (C4d),  and epidermal transglutaminase (transglutaminase 3, TG3)

Eosinophil granule proteins: Eosinophil granule major basic protein 1 (eMBP1) and eosinophil-derived neurotoxin (EDN)

Serum Specimens

Antibody Panels

  • Immunobullous Disease Antibody Panel
  • Basement Membrane Zone Antibody Panel
  • Epidermolysis Bullosa Acquisita Antibody Panel
  • Pemphigoid Antibody Panel
  • Pemphigoid Gestationis Panel
  • Pemphigus IgG Antibody Panel
  • Pemphigus IgG & IgA Antibody Panel
  • Paraneoplastic Pemphigus (Paraneoplastic Autoimmune Multiorgan Syndrome) Expanded IgG Antibody Panel by Indirect Immunofluorescence with Envoplakin ELISA (includes rat bladder substrate; IgA indirect immunofluorescence by request)
  • Paraneoplastic Pemphigus (Paraneoplastic Autoimmune Multiorgan Syndrome) Screening IgG Antibodies by Indirect Immunofluorescence (includes rat bladder substrate; does NOT include IgG envoplakin ELISA; IgA indirect immunofluorescence by request)
  • Dermatitis Herpetiformis IgA Antibody Panel
  • Dermatitis Herpetiformis IgA & IgG Antibody Panel
  • Celiac Disease IgA Antibody Panel
  • Celiac Disease IgA & IgG Antibody Panel
  • Celiac Disease / Dermatitis Herpetiformis IgG Antibody Panel

 

Antibody panel testing is most useful for initial diagnostic testing, changing disease presentations, and periodically evaluating antibody expression profiles in established diseases. Antibody Panels are comprised of various combinations of Specific Antibody Assays and provide the broadest assessment of disease-related autoantibody expression.

Please contact us if you have any questions about our testing.

Immunobullous Disease Antibody Panel Components

Indirect Immunofluorescence

  • IgG & IgA (Epithelial) Basement Membrane Zone Antibodies, including split skin / salt split skin substrate
  • IgG & IgA (Epithelial) Cell Surface / Intercellular Substance (ICS) Antibodies
  • IgG Laminin-332 Antibodies (in validation process, included if requested, especially for mucosal involvement)

ELISA

  • IgG BP180 & IgG BP230 Antibodies
  • IgG Type VII Collagen Antibody
  • IgG Desmoglein 1 & IgG Desmoglein 3 Antibodies

Basement Membrane Zone Antibody Panel Components

Indirect Immunofluorescence

  • IgG & IgA (Epithelial) Basement Membrane Zone Antibodies, including split skin / salt split skin substrate
  • IgG Laminin-332 Antibodies (in validation process, included if requested, especially for mucosal involvement)

ELISA

  • IgG BP180 & IgG BP230 Antibodies
  • IgG Type VII Collagen Antibody

Dermatitis Herpetiformis IgA Antibody Panel Components

Indirect Immunofluorescence

  • IgA  Endomysial Antibodies

ELISA

  • IgA & IgG Tissue Transglutaminase (tTG / TG2) Antibodies
  • IgA Epidermal Transglutaminase (eTG / TG3) Antibody

Learn more

Pemphigus IgG Antibody Panel Components

Indirect Immunofluorescence

  • IgG (Epithelial) Cell Surface / Intercellular Substance (ICS) Antibodies

ELISA

  • IgG Desmoglein 1 & IgG Desmoglein 3 Antibodies

Pemphigoid Antibody Panel Components

Indirect Immunofluorescence

  • IgG & IgA (Epithelial) Basement Membrane Zone Antibodies, including split skin / salt split skin substrate

ELISA

  • IgG BP180 & IgG BP230 Antibodies

Learn more

Dermatitis Herpetiformis IgA & IgG Antibody Panel Components

Indirect Immunofluorescence

  • IgA & IgG Endomysial Antibodies

ELISA

  • IgA & IgG Tissue Transglutaminase (tTG / TG2) Antibodies
  • IgA Epidermal Transglutaminase (eTG / TG3) Antibody

Pemphigus IgG & IgA Antibody Panel Components

Indirect Immunofluorescence

  • IgG & IgA (Epithelial) Cell Surface / Intercellular Substance (ICS) Antibodies

ELISA

  • IgG Desmoglein 1 & IgG Desmoglein 3 Antibodies

Epidermolysis Bullosa Acquisita Antibody Panel Components

Indirect Immunofluorescence

  • IgG & IgA (Epithelial) Basement Membrane Zone Antibodies, including split skin/salt split skin substrate

ELISA

  • IgG Type VII Collagen Antibody

Learn more

Celiac Disease IgA Antibody Panel Components

Indirect Immunofluorescence

  • IgA  Endomysial Antibodies

ELISA

  • IgA Tissue Transglutaminase (tTG / TG2) Antibodies

Paraneoplastic Pemphigus Antibody Panel Components

(Paraneoplastic Autoimmune Multiorgan Syndrome)

Indirect Immunofluorescence

  • IgG (Epithelial) Cell Surface & Basement Membrane Zone Antibodies on rat and mouse bladders and other rodent substrates including mouse heart and liver

ELISA

  • IgG Envoplakin Antibodies (in validation process, included if requested)

Pemphigoid Gestationis Panel Components

Indirect Immunofluorescence

  • Complement-Fixing Basement Membrane Zone Antibodies (herpes gestationis factor)
  • IgG & IgA (Epithelial) Basement Membrane Zone Antibodies, including split skin/salt split skin substrate

ELISA

  • IgG BP180 & IgG BP230 Antibodies

Celiac Disease IgA & IgG Antibody Panel Components

Indirect Immunofluorescence

  • IgA & IgG Endomysial Antibodies

ELISA

  • IgA & IgG Tissue Transglutaminase (tTG / TG2) Antibodies

Celiac Disease / Dermatitis Herpetiformis IgG Antibody Panel Components

Indirect Immunofluorescence

  • IgG Endomysial Antibodies

ELISA

  • IgG Tissue Transglutaminase (tTG/TG2) Antibodies
testing samples

Board Certified

John J. Zone, M.D.,  Kristin M. Leiferman, M.D., and Melanie K. Kuechle, M.D. are American Board of Dermatology Diplomats in Dermatology and Dermatological Immunology / Diagnostic and Laboratory Immunology. Mazdak A. Khalighi,  M.D. and Margaret M. Cocks, Ph.D., M.D. are American Board of Pathology Diplomats in Anatomic Pathology. Dr. Khalighi additionally has fellowship training and faculty experience in Renal Pathology/Immunopathology. Dr. Cocks additionally is an American Board of Pathology Diplomat in Clinical Pathology and is an American Board of Dermatology Diplomat in Dermatology and Dermatopathology. Drs. Khalighi and Cocks have had specialized, fellowship-equivalent training in laboratory immunodermatology by Drs. Zone and Leiferman.