IMMUNODERMATOLOGY LABORATORY TEST MENU
The Immunodermatology Laboratory offers tests on skin and mucosal tissue specimens and serum specimens that aid in the diagnosis and management of many types of dermatological conditions and diseases. Tissue testing is more sensitive than serum testing in several diseases, and the findings from tissue testing may prompt serum testing to distinguish certain diseases and establish antibody levels for therapeutic tracking. Serum testing includes Antibody Panels and Specific Antibody Assays to identify disease-related immunological markers and patterns of autoantibody reactivity. Serum Antibody Panel Testing is most useful for initial diagnostic testing, assessing changing disease presentations, and periodically evaluating antibody expression profiles in established diseases. Specific antibody assays may be helpful in the diagnosis of certain diseases and indicated for monitoring disease activity. Of note, ELISA testing detects serum antibodies to specific epitopes in major target antigens, and patients may develop antibodies to other epitopes or other epithelial antigens; therefore, the combination of indirect immunofluorescence and ELISA testing can provide more comprehensive immunopathological information that either alone.
Please contact us if you have any questions about our testing.
Tissue Specimens
Direct immunofluorescence (DIF): IgG, IgG4, IgM, IgA, C3, and fibrinogen; upon request other IgG subclasses (IgG1, IgG2, IgG3), another complement component (C4d), and epidermal transglutaminase (transglutaminase 3, TG3)
Eosinophil granule proteins: Eosinophil granule major basic protein 1 (eMBP1) and eosinophil-derived neurotoxin (EDN)
Serum Specimens
- Immunobullous Disease Antibody Panel
- Basement Membrane Zone Antibody Panel
- Epidermolysis Bullosa Acquisita Antibody Panel
- Pemphigoid Antibody Panel
- Pemphigoid Gestationis Panel
- Pemphigus IgG Antibody Panel
- Pemphigus IgG & IgA Antibody Panel
- Paraneoplastic Pemphigus (Paraneoplastic Autoimmune Multiorgan Syndrome) Expanded IgG Antibody Panel by Indirect Immunofluorescence with Envoplakin ELISA (includes rat bladder substrate; IgA indirect immunofluorescence by request)
- Paraneoplastic Pemphigus (Paraneoplastic Autoimmune Multiorgan Syndrome) Screening IgG Antibodies by Indirect Immunofluorescence (includes rat bladder substrate; does NOT include IgG envoplakin ELISA; IgA indirect immunofluorescence by request)
- Dermatitis Herpetiformis IgA Antibody Panel
- Dermatitis Herpetiformis IgA & IgG Antibody Panel
- Celiac Disease IgA Antibody Panel
- Celiac Disease IgA & IgG Antibody Panel
- Celiac Disease / Dermatitis Herpetiformis IgG Antibody Panel
- Indirect Immunofluorescence
- IgG basement membrane zone antibodies
- IgG laminin-332 antibodies (in validation process, by request)
- IgA basement membrane zone antibodies
- IgM basement membrane zone antibodies (by request)
- IgA cell surface / intercellular substance (ICS) antibodies (IgA Pemphigus)
- IgG cell surface / intercellular substance (ICS) antibodies
- IgA endomysial antibodies (EMA)
- IgG endomysial antibodies (EMA)
- Enzyme-Linked Immunosorbent Assay (ELISA)
- IgG BP180 and IgG BP230 antibody levels
- IgG type VII collagen antibody level
- IgG desmoglein 1 and IgG desmoglein 3 antibody levels
- IgA tissue transglutaminase (tTG) / transglutaminase 2 (TG2) antibody level
- IgG tissue transglutaminase (tTG) / transglutaminase 2 (TG2) antibody level
- IgA epidermal transglutaminase (eTG) / transglutaminase 3 (TG3) antibody Level
- IgG Envoplakin Antibody Level by ELISA
Antibody panel testing is most useful for initial diagnostic testing, changing disease presentations, and periodically evaluating antibody expression profiles in established diseases. Antibody Panels are comprised of various combinations of Specific Antibody Assays and provide the broadest assessment of disease-related autoantibody expression.
Please contact us if you have any questions about our testing.
Indirect Immunofluorescence
- IgG & IgA (Epithelial) Basement Membrane Zone Antibodies, including split skin / salt split skin substrate
- IgG & IgA (Epithelial) Cell Surface / Intercellular Substance (ICS) Antibodies
- IgG Laminin-332 Antibodies (in validation process, included if requested, especially for mucosal involvement)
ELISA
- IgG BP180 & IgG BP230 Antibodies
- IgG Type VII Collagen Antibody
- IgG Desmoglein 1 & IgG Desmoglein 3 Antibodies
Indirect Immunofluorescence
- IgG & IgA (Epithelial) Basement Membrane Zone Antibodies, including split skin / salt split skin substrate
- IgG Laminin-332 Antibodies (in validation process, included if requested, especially for mucosal involvement)
ELISA
- IgG BP180 & IgG BP230 Antibodies
- IgG Type VII Collagen Antibody
Indirect Immunofluorescence
- IgA Endomysial Antibodies
ELISA
- IgA & IgG Tissue Transglutaminase (tTG / TG2) Antibodies
- IgA Epidermal Transglutaminase (eTG / TG3) Antibody
Indirect Immunofluorescence
- IgG (Epithelial) Cell Surface / Intercellular Substance (ICS) Antibodies
ELISA
- IgG Desmoglein 1 & IgG Desmoglein 3 Antibodies
Indirect Immunofluorescence
- IgG & IgA (Epithelial) Basement Membrane Zone Antibodies, including split skin / salt split skin substrate
ELISA
- IgG BP180 & IgG BP230 Antibodies
Indirect Immunofluorescence
- IgA & IgG Endomysial Antibodies
ELISA
- IgA & IgG Tissue Transglutaminase (tTG / TG2) Antibodies
- IgA Epidermal Transglutaminase (eTG / TG3) Antibody
Indirect Immunofluorescence
- IgG & IgA (Epithelial) Cell Surface / Intercellular Substance (ICS) Antibodies
ELISA
- IgG Desmoglein 1 & IgG Desmoglein 3 Antibodies
Indirect Immunofluorescence
- IgG & IgA (Epithelial) Basement Membrane Zone Antibodies, including split skin/salt split skin substrate
ELISA
- IgG Type VII Collagen Antibody
Indirect Immunofluorescence
- IgA Endomysial Antibodies
ELISA
- IgA Tissue Transglutaminase (tTG / TG2) Antibodies
Indirect Immunofluorescence
- IgG (Epithelial) Cell Surface & Basement Membrane Zone Antibodies on rat and mouse bladders and other rodent substrates including mouse heart and liver
ELISA
- IgG Envoplakin Antibodies (in validation process, included if requested)
Indirect Immunofluorescence
- Complement-Fixing Basement Membrane Zone Antibodies (herpes gestationis factor)
- IgG & IgA (Epithelial) Basement Membrane Zone Antibodies, including split skin/salt split skin substrate
ELISA
- IgG BP180 & IgG BP230 Antibodies
Indirect Immunofluorescence
- IgA & IgG Endomysial Antibodies
ELISA
- IgA & IgG Tissue Transglutaminase (tTG / TG2) Antibodies
Indirect Immunofluorescence
- IgG Endomysial Antibodies
ELISA
- IgG Tissue Transglutaminase (tTG/TG2) Antibodies
Board Certified
John J. Zone, M.D., Kristin M. Leiferman, M.D., and Melanie K. Kuechle, M.D. are American Board of Dermatology Diplomats in Dermatology and Dermatological Immunology / Diagnostic and Laboratory Immunology. Mazdak A. Khalighi, M.D. and Margaret M. Cocks, Ph.D., M.D. are American Board of Pathology Diplomats in Anatomic Pathology. Dr. Khalighi additionally has fellowship training and faculty experience in Renal Pathology/Immunopathology. Dr. Cocks additionally is an American Board of Pathology Diplomat in Clinical Pathology and is an American Board of Dermatology Diplomat in Dermatology and Dermatopathology. Drs. Khalighi and Cocks have had specialized, fellowship-equivalent training in laboratory immunodermatology by Drs. Zone and Leiferman.
Contact Us
Immunodermatology Laboratory,
Department of Dermatology
University of Utah Health
417 South Wakara Way, Suite 2151
Salt Lake City, UT 84108