Ordering Recommendation
• This is a test for autoantibodies in patient’s serum for diagnostic assessment and disease monitoring in celiac disease and dermatitis herpetiformis.
• For initial diagnosis of dermatitis herpetiformis, order concurrently with perilesional skin biopsy for direct immunofluorescence.
• Use for disease monitoring with semiquantitative antibody level assessments and tracking.
• Consider more broad-based panel autoantibody assessment initially and for persisting unexplained or worsening disease activity; panels that include IgA endomysial antibodies: Dermatitis Herpetiformis IgA Panel and Celiac Disease IgA Panel.
Methodology
Indirect immunofluorescence - monkey esophagus substrate (additionally, human umbilical cord substrate, as indicated)
Performed
Daily
Reported
2-5 days
New York State Department of Health (NYSDOH) Approval Status
This test is New York State DOH approved.
Information Required to Submit with Order
Completed Immunodermatology Laboratory requisition form and copies of both sides of insurance cards or completed billing information.
CPT Code(s)
88346 (86256 x 1 possible)
SPECIMEN REQUIREMENTS
Collect Blood
Plain red top tube or serum separator tube.
Specimen Preparation
2 mL serum separated from blood into serum vial provided in kit (Minimum needed: 0.5 mL)
Storage/Transport Temperature
Room temperature
Conditions with Disclaimer for Potentially Anomalous Results
Hemolyzed or lipemic serum specimens, whole blood
Stability
Ambient: 7 days
Refrigerated: 14 days
Frozen: Indefinitely