Ordering Recommendation
• This is a secondary autoantibody test for diagnostic assessment and disease monitoring in celiac disease and dermatitis herpetiformis for patients with low total serum IgA and for patients with the suspected rare variant disease with normal IgA serum level but without positive IgA endomysial or increased IgA tissue transglutaminase antibodies.
• Order concurrently with perilesional skin biopsy for direct immunofluorescence for initial diagnosis of dermatitis herpetiformis.
• Use for disease monitoring with semiquantitative antibody level assessments and tracking.
Methodology
Indirect immunofluorescence - monkey esophagus substrate (additionally, human umbilical cord substrate, as indicated)
Performed
Daily
Reported
2-5 days
New York State Department of Health (NYSDOH) Approval Status
This test is New York State DOH approved.
Information Required to Submit with Order
Completed Immunodermatology Laboratory requisition form and copies of both sides of insurance cards or completed billing information.
CPT Code(s)
88346 (86256 x 1 possible)
SPECIMEN REQUIREMENTS
Collect Blood
Plain red top tube or serum separator tube.
Specimen Preparation
2 mL serum separated from blood into serum vial provided in kit (Minimum needed: 0.5 mL)
Storage/Transport Temperature
Room temperature
Conditions with Disclaimer for Potentially Anomalous Results
Hemolyzed or lipemic serum specimens, whole blood
Stability
Ambient: 7 days
Refrigerated: 14 days
Frozen: Indefinitely