Eosinophil Granule Proteins

Eosinophil Granule Proteins:

Eosinophil granule major basic protein 1 (eMBP1) and eosinophil-derived neurotoxin (EDN)

Ordering Recommendation

- Order to assess eosinophil involvement in pathophysiology, particularly if eosinophil degranulation may be playing an unrecognized role because intact cells are not morphologically identifiable by routine histopathology.

Information Required to Submit with Order

Completed Immunodermatology Laboratory requisition form and copies of both sides of insurance cards or completed billing information

Methodology

Tissue indirect immunofluorescence – for eosinophil granule major basic protein 1 (eMBP1) and eosinophil-derived neurotoxin (EDN)

Performed

Varies, weekly to longer intervals

Reported

Varies, within 3 days of performing test

CPT Code(s)

88305, 88341, 88342

SPECIMEN REQUIREMENTS

Collect

Tissue suspected of eosinophil involvement (not blood or body fluids): skin, mucous membrane (oral, genital, ocular), gastrointestinal tract, respiratory tract, solid organ, etc.

Specimen Preparation

Transport in Michel's medium or Zeus tissue fixative. Order complimentary specimen kit(s).  Also acceptable: formalin fixative, tissue in paraffin block, and slides with tissue sections. Contact Immunodermatology Laboratory for sectioning instructions if sending paraffin-embedded tissue sectioned on slides. Also acceptable: frozen.

Storage/Transport Temperature

Room temperature.  

Unacceptable Conditions

Glutaraldehyde and other fixatives than those in Specimen Preparation section above.

Stability

For Michel’s or Zeus’ transport:
Ambient: 10 days
Refrigerated: 10 days
Formalin fixative, paraffin-embedded tissue, sectioned fixed tissue on slides: indefinitely
Frozen: indefinitely, only if frozen immediately and kept in that state, transported on dry ice or in liquid nitrogen