Ordering Recommendation
• This is an autoantibody panel for initial diagnostic assessment and disease monitoring in in disorders that present with blistering and erosions, which affect skin and mucous membranes, from suspected epidermolysis bullosa acquisita.
• Order concurrently with perilesional skin biopsy for direct immunofluorescence for initial diagnosis.
• Use for disease monitoring with semiquantitative antibody level assessments and tracking
• To screen for other immunobullous diseases in addition to epidermolysis bullosa acquisita, order concurrently with the Pemphigus Panel, IgA cell surface antibodies indirect immunofluorescence individual test, IgG BP 180 and IgG BP 230 antibody ELISAs individual test, Dermatitis Herpetiformis IgA Panel.
Components
- IgA basement membrane zone antibodies by indirect immunofluorescence
- IgG basement membrane zone antibodies by indirect immunofluorescence
- IgG collagen VII antibody level by ELISA
Methodology
Indirect immunofluorescence and enzyme-linked immunosorbent assay (ELISA)
Performed
Daily
Reported
4-9 business days
New York Department of Health (NYDOH) Approval Status
This test is New York DOH approved except the IgG Collagen VII ELISA.
Information Required to Submit with Order
Completed Immunodermatology Laboratory requisition form and copies of both sides of insurance cards or completed billing information
CPT Code(s)
88346; 88350 x 3; 83516 (86256 x 1-4 possible)
SPECIMEN REQUIREMENTS
Collect Blood
Plain red top tube or serum separator tube.
Specimen Preparation
2 mL serum separated from blood into serum vial provided in kit (Minimum needed: 0.5 mL)
Storage/Transport Temperature
Room temperature
Conditions with Disclaimer for Potentially Anomalous Results
Hemolyzed or lipemic serum specimens, whole blood
Stability
Ambient: 7 days
Refrigerated: 14 days
Frozen: Indefinitely