Ordering Recommendation
• This is a broad epithelial autoantibody panel for initial diagnostic assessment in disorders that present with blistering, erosions, pruritus, and/or urticaria, which affect skin and mucous membranes including pemphigoid, pemphigoid variants, epidermolysis bullosa acquisita, linear IgA bullous dermatosis, linear IgA disease variants, and IgG-pemphigus subtypes.
• Order concurrently with Dermatitis Herpetiformis IgA Panel for broadest screening to include assessment for dermatitis herpetiformis
• Order concurrently with perilesional skin biopsy for direct immunofluorescence for initial diagnosis.
• Use for disease monitoring with semiquantitative antibody level assessments and tracking and for persistent unexplained disease and/or worsening disease activity because antibody profiles and levels may shift
Components
- IgG basement membrane zone antibodies by indirect immunofluorescence
- IgA basement membrane zone antibodies by indirect immunofluorescence
- IgG BP 180 and IgG BP 230 antibody levels by ELISAs
- IgG collagen VII antibody level by ELISA
- IgG cell surface antibodies by indirect immunofluorescence
- IgA cell surface antibodies by indirect immunofluorescence
- IgG desmoglein 1 and IgG desmoglein 3 antibody levels by ELISAs
Methodology
Indirect immunofluorescence and enzyme-linked immunosorbent assay (ELISA)
Performed
Daily
Reported
4-9 days
New York Department of Health (DOH) Approval Status
This test is New York DOH approved except the IgG Collagen VII ELISA.
Information Required to Submit with Order
Completed Immunodermatology Laboratory requisition form and copies of both sides of insurance cards or completed billing information
CPT Code(s)
88346; 88350 x 5; 83516 x 5 (86256 x 1-6 possible)
SPECIMEN REQUIREMENTS
Collect Blood
Plain red top tube or serum separator tube.
Specimen Preparation
2 mL serum separated from blood into serum vial provided in kit (Minimum needed: 0.5 mL)
Storage/Transport Temperature
Room temperature
Conditions with Disclaimer for Potentially Anomalous Results
Hemolyzed or lipemic serum specimens, whole blood
Stability
Ambient: 7 days
Refrigerated: 14 days
Frozen: Indefinitely