Paraneoplastic Pemphigus IgG Antibodies

Ordering Recommendation

• For initial diagnostic assessment and disease monitoring in paraneoplastic pemphigus

•  For best sensitivity in initial diagnosis, order concurrently with perilesional skin biopsy, when possible to obtain, for direct immunofluorescence

 • For variant presentations, IgA Paraneoplastic Pemphigus Antibody testing can be requested

 • Consider ordering with IgG Desmoglein 1 and IgG desmoglein 3 Antibody Levels or Pemphigus Panel and IgG BP 180 and IgG BP 230 Antibody Levels because pemphigus vulgaris may have similar clinical features and also because serum antibodies to multiple epithelial (simple, columnar, transitional) antigens may develop in paraneoplastic pemphigus including desmoglein 1, 3; desmoplakin 1, 2; envoplakin; periplakin; BP 180 and/or BP 230

Methodology

Indirect immunofluorescence - mouse liver, mouse heart, mouse bladder, and rat bladder substrates (when this test is ordered alone, monkey esophagus substrate also is included in the testing)

Performed

Daily

Reported

2-5 days

New York Department of Health (NYDOH) Approval Status

This test is New York DOH approved.

Information Required to Submit with Order

Completed Immunodermatology Laboratory requisition form and copies of both sides of insurance cards or completed billing information.

CPT Code(s)

88346; 88350 x 4 (86256 x 4 possible)

SPECIMEN REQUIREMENTS

Collect Blood

Plain red top tube or serum separator tube.  

Specimen Preparation

2 mL serum separated from blood into serum vial provided in kit (Minimum needed: 0.5 mL) 

Storage/Transport Temperature

Room temperature  

Conditions with Disclaimer for Potentially Anomalous Results

Hemolyzed or lipemic serum specimens, whole blood  

Stability

Ambient: 7 days
Refrigerated: 14 days
Frozen: Indefinitely