Pemphigus Panel
Ordering Recommendation
• This is an autoantibody panel for initial diagnostic assessment and disease monitoring in suspected IgG-subtype pemphigus. Pemphigus vulgaris and pemphigus foliaceus are the most common types of pemphigus with blistering and erosive disease affecting skin and mucous membranes. Specific reactivity to the type of desmoglein distinguishes pemphigus subtypes; IgG desmoglein 1 autoantibodies predominate in patients with pemphigus foliaceus, and IgG desmoglein 3 autoantibodies, with or without accompanying desmoglein 1 autoantibodies, predominate in patients with pemphigus vulgaris.
• Order concurrently with perilesional skin biopsy for direct immunofluorescence for initial diagnosis.
• Use for disease monitoring with semiquantitative antibody level assessments and tracking
• To screen for pemphigus along with other possible immunobullous diseases, order concurrently with Pemphigoid Panel, IgG collagen type VII antibody, Dermatitis Herpetiformis IgA Panel.
Components
- IgG cell surface antibodies by indirect immunofluorescence
- IgG desmoglein 1 and IgG desmoglein 3 antibody levels by ELISAs
Methodology
Indirect immunofluorescence and enzyme-linked immunosorbent assay (ELISA)
Performed
Daily
Reported
2-7 business days
New York Department of Health (NYDOH) Approval Status
This test is New York DOH approved.
Information Required to Submit with Order
Completed Immunodermatology Laboratory requisition form and copies of both sides of insurance cards or completed billing information
CPT Code(s)
88346; 88350 x 1; 83516 x 2 (86256 x 1-2 possible)
SPECIMEN REQUIREMENTS
Collect Blood
Plain red top tube or serum separator tube.
Specimen Preparation
2 mL serum separated from blood into serum vial provided in kit (Minimum needed: 0.5 mL)
Storage/Transport Temperature
Room temperature
Conditions with Disclaimer for Potentially Anomalous Results
Hemolyzed or lipemic serum specimens, whole blood
Stability
Ambient: 7 days
Refrigerated: 14 days
Frozen: Indefinitely