Ordering Recommendation
- For disease activity monitoring in pemphigoid. Serum levels of IgG BP 180 antibodies correlate with disease activity in some cases of pemphigoid; best for monitoring patients with known increased levels
- Patients with pemphigoid may show reactivity to multiple basement membrane zone components in addition to or other than the BP 180 and BP 230 epitopes expressed in these ELISAs, and patients with epidermolysis bullosa acquisita may have a similar clinical presentation with positive IgG basement membrane zone antibodies; therefore, consider more broad-based panel autoantibody assessment initially and for persisting unexplained or worsening disease activity because antibody profiles and levels may evolve over time: Pemphigoid Panel and IgG Collagen VII Antibody Level or Basement Membrane Zone Antibody Panel
Methodology
Enzyme-linked immunosorbent assays (ELISA)
Performed
2-3 times weekly
Reported
2-5 days
New York State Department of Health (NYSDOH) Approval Status
This test is New York State DOH approved.
Information Required to Submit with Order
Completed Immunodermatology Laboratory requisition form and copies of both sides of insurance cards or completed billing information.
CPT Code(s)
83516 x 2
SPECIMEN REQUIREMENTS
Collect Blood
Plain red top tube or serum separator tube.
Specimen Preparation
2 mL serum separated from blood into serum vial provided in kit (Minimum needed: 1.0 mL)
Storage/Transport Temperature
Room temperature
Conditions with Disclaimer for Potentially Anomalous Results
Hemolyzed or lipemic serum specimens, whole blood
Stability
Ambient: 7 days
Refrigerated: 14 days
Frozen: Indefinitely